Influence of N-No3 and N-Nh4 on the Mineral Composition of Grape-Vine Rootstocks Cultured in Vitro.

Villegas, A.; Mazuelos, C.; Troncoso, A.

doi:10.17660/actahortic.1992.300.13

Cited by 3 publications

(3 citation statements)

References 2 publications

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“…The increase was moderate for KNO3, more marked for ( N H 4 ) 2 S O 4 , and maximal for NHaNO 3. These data are in agreement with previous results of Troncoso et al (1990) and Villegas et al (1990). With KNO 3, the moderate increase in total N indicates a highly controlled absorption process, probably related to the high energy and carbon skeleton requirement for re- duction by nitrate reductase and subsequent assimilation (Campbell, 1988;Stewart and Rhodes, 1977).…”

Section: Resultssupporting

confidence: 92%

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Influence of the nitrogen source and concentration on N fractions and free amino acid levels of grape vine explants

et al. 1992

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The influence of the source of inorganic nitrogen (KNO3, ( N H 4 ) 2 S O 4 and NH4NO3) and its concentration (5, 10, 20 and 30 mM N) on total N incorporation, as well as on N distribution into different fractions (ammoniacal, amino, amide and protein) and on free amino acid levels has been determined in grape vine explants cultured in vitro.Increasing concentrations of the nitrogen source resulted in increased total N content in tissues. This effect was small for KNO3, higher for (NH4)2SO 4 and maximal for NHaNO 3. In addition, nitrate promoted an increase in amino-N only, whereas ammonium increased both the ammoniacal-N and the amino-N fractions. Incorporation of N into amide-N and protein-N were not affected significantly by the N sources tested.The application of increasing quantities of N enhanced the accumulation of most free amino acids, especially arginine, alanine and proline, but to different extents, depending on both the N source and its concentration. The combination of ammonium and nitrate resulted in a higher accumulation of amino acids than that observed with either one of the two forms alone.

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Section: Resultssupporting

confidence: 92%

“…Previous work on the nitrogen nutrition of explants of grape vine rootstocks Villegas et al, 1990) has revealed differential effects on shoot and callus development. Increasing ammonium resulted in a significant increase in number, size and N content of shoots, and decreased callus formation.…”

Section: Introductionmentioning

confidence: 99%

Influence of the nitrogen source and concentration on N fractions and free amino acid levels of grape vine explants

et al. 1992

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“…Consequently, this substrate may not be optimal for certain species [15,25] and can be improved, as shown for persimmon [26] and grapevine [27]. According to Chée and Pool, KI is a non-essential component for the growth and development of grapevine plants and can subsequently be removed from tissue culture media [28].…”

Section: Introductionmentioning

confidence: 99%

Effects of Culture System and Substrate Composition on Micropropagated Plantlets of Two Varieties of Stevia rebaudiana Bert

et al. 2023

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Background: Secondary metabolites in stevia leaves offer important therapeutic benefits. The germination problems of stevia seeds and the high heterogeneity of the resulting populations make micropropagation the preferred tool for obtaining a large number of plants in a short time. Until now, studies have focused on optimizing the action of growth regulators for the improvement of stevia micropropagated plantlets. In this project, we study alternative mineral nutrients in the substrate and two culture systems on micropropagated stevia plantlets to obtain proper amounts of enhanced plantlets. Methods: Criolla and Morita varieties; MS, MSM, and G substrates, and temporary immersion (TIS) and agar-medium systems were used. Biometric variables and damage to the cell membrane of the resulting plantlets were tested. Results: More productivity in the Criolla plantlets growing in MS solid medium and TIS. The Morita plantlets presented higher productivity in the solid MS, MSM, and G substrates and TIS. The explants growing in MS and MSM presented the highest productivity. The solid substrate was generally better than TIS. No damage to the cell membrane was found. Conclusions: The micropropagation efficiency of stevia plantlets of the two varieties has been enhanced without negative effects on their physiological condition. Consequently, any of the culture systems used, including bioreactors, can be of industrial application on a large scale.

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Polyembryony and identification of Volkamerian lemon zygotic and nucellar seedlings using RAPD

Andrade-Rodríguez

Villegas-Monter

Carrillo-Castañeda

et al. 2004

Pesq. agropec. bras.

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-The objectives of this work were to evaluate the frequency of polyembryony, and to identify zygotic and nucellar seedlings of Citrus volkameriana using RAPD. Twenty-five polyembryonic and eight monoembryonic seeds were cultivated in vitro for six months. DNA from seedlings was extracted and used in combination with five RAPD primers to identify zygotic or nucellar origin of the seedlings. Environmental conditions of the year affected significantly (P≤0.05) the morphological characteristics of fruits and the number of embryos per seed. Polyembryonic seeds ranged from 30.9%, 44.8% to 54.4% over three years. Morphological characteristic was not correlated with polyembryony. In vitro culture enable all embryos of each seed to grow, favoring the percentage of seedlings identified as zygotic. In polyembryonic and monoembryonic seeds, 25.9% and 87.5% of the seedlings, respectively, were sexually originated. In polyembryonic seeds, not all zygotic seedlings were produced by small embryos located at the micropyle.Index terms: Citrus volkameriana, rootstock, embryo culture, molecular markers, polymorphism. Termos para indexação: Citrus volkameriana, porta-enxerto, cultura de embriões, marcadores moleculares, polimorfismo. Poliembrionia e identificação de seedlings zigóticos e nucelares de limão volkameriano com RAPD

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Influence of N-No3 and N-Nh4 on the Mineral Composition of Grape-Vine Rootstocks Cultured in Vitro.

Cited by 3 publications

References 2 publications

Influence of the nitrogen source and concentration on N fractions and free amino acid levels of grape vine explants

Influence of the nitrogen source and concentration on N fractions and free amino acid levels of grape vine explants

Effects of Culture System and Substrate Composition on Micropropagated Plantlets of Two Varieties of Stevia rebaudiana Bert

Polyembryony and identification of Volkamerian lemon zygotic and nucellar seedlings using RAPD

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