Influence of nanomaterials on stem cell differentiation: designing an appropriate nanobiointerface

Mocan, Lucian; Ilie,; Mocan, Teodora

doi:10.2147/ijn.s29975

Cited by 34 publications

(9 citation statements)

References 89 publications

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“…In this regard, nanomaterials can be classified into organic and inorganic systems. Diversity in organic materials derived mainly from polymers, such as polysaccharides, collagen, and chitosan have been recently used with different morphologies into the biomedical application and stem cell differentiation [19]. In particular, the use of polymer NPs as carriers or drug delivery systems is promising materials used as neuroprotectors to avoid acute ischemic stroke, which is actually considered one of the most common causes of death worldwide [40].…”

Section: Bio-nanocompositesmentioning

confidence: 99%

“…Recent advances in the use of nano-sized particles in pharmaceutics involves the design of controlled drug delivery systems [16], biomarkers as diseases detection [2], pathogen/protein identification [17], molecule separation/purification [18] and regenerative medicine approaches [17,19,20]. Recent studies have been focused in biomedicine in order to execute multidisciplinary research, combining topics such as chemistry, biology, physics, engineering and materials science; associated with the design of functional systems, addressed to the tissue regeneration responses in organisms.…”

Section: Introductionmentioning

confidence: 99%

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Trends in Tissue Regeneration: Bio-Nanomaterials

España‐Sánchez¹,

Cruz²,

Elizalde-Peña³

et al. 2018

Tissue Regeneration

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Tissue engineering requires functional platforms or scaffolds with specific properties concerning the morphology, chemistry of the surface and interconnectivity to promote cell adhesion and proliferation. These requisites are not only important for cellular migration but also to supply nutrients and expulsion of waste molecules. Cell type must be considered when designing a specific cellular grown system as a scaffold; for instance, if they are autologous, allogeneic or xenogeneic. The challenge in tissue engineering is to develop an organized three-dimensional architecture with functional characteristics that mimic the extracellular matrix. In this regard, with the advent of nanotechnology scaffolds are now being developed that meet most of the aforementioned requisites. In the present chapter, the use of biopolymers based nanostructures is addressed, including biomaterials and stem cells, bio-nanocomposites, and specific clinical cases where these systems were employed. We emphasize the future challenges and perspectives in the design of biocompatible and nontoxic nanocomposites with high efficiency as a promoter for tissue regeneration and many other biomedical applications.

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Section: Bio-nanocompositesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Trends in Tissue Regeneration: Bio-Nanomaterials

España‐Sánchez¹,

Cruz²,

Elizalde-Peña³

et al. 2018

Tissue Regeneration

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show abstract

“…Nanotechnology is able to direct the differentiation of stem cells, by delivering growth factors, as bioactive molecules, that trigger specific signaling pathways [ 62 , 68 ]. Retina consists of many structures containing highly specialized cells, such as sensory cells or specific neurons, bipolar cells, retinal ganglion cells, and amacrine cells.…”

Section: Nanotechnology As Tool For the Improvement Of Stem Cells'mentioning

confidence: 99%

Novel Strategies for the Improvement of Stem Cells’ Transplantation in Degenerative Retinal Diseases

Nicoară

Şuşman

Tudoran

et al. 2016

Stem Cells International

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Currently, there is no cure for the permanent vision loss caused by degenerative retinal diseases. One of the novel therapeutic strategies aims at the development of stem cells (SCs) based neuroprotective and regenerative medicine. The main sources of SCs for the treatment of retinal diseases are the embryo, the bone marrow, the region of neuronal genesis, and the eye. The success of transplantation depends on the origin of cells, the route of administration, the local microenvironment, and the proper combinative formula of growth factors. The feasibility of SCs based therapies for degenerative retinal diseases was proved in the preclinical setting. However, their translation into the clinical realm is limited by various factors: the immunogenicity of the cells, the stability of the cell phenotype, the predilection of SCs to form tumors in situ, the abnormality of the microenvironment, and the association of a synaptic rewiring. To improve SCs based therapies, nanotechnology offers a smart delivery system for biomolecules, such as growth factors for SCs implantation and differentiation into retinal progenitors. This review explores the main advances in the field of retinal transplantology and applications of nanotechnology in the treatment of retinal diseases, discusses the challenges, and suggests new therapeutic approaches in retinal transplantation.

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“…10 At such a small scale, the physical, chemical, and biological properties of materials are often quite different, which has brought unprecedented possibilities for its usage in the field of drug delivery, imaging, diagnostics, and other medical applications, especially stem cell research. [11][12][13] Over the past few decades, researches on graphene have been increasing rapidly for applications in fields of nanomaterials and nanotechnology, especially in tissue engineering and regenerative medicine. 14 Graphene is a two-dimensional nanoparticle comprising of a single layer with six atoms arranged in a honeycomb lattice in sp 2 hybridization.…”

Section: Introductionmentioning

confidence: 99%

<p>Size-Dependent Effects of Suspended Graphene Oxide Nanoparticles on the Cellular Fate of Mouse Neural Stem Cells</p>

Lin

Zhuang

Huang

et al. 2020

IJN

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These authors contributed equally to this workPurpose: In this study, we aim to explore the effects of graphene oxide (GO), a derivative of graphene, nanoparticles of four different sizes on the cellular fate of mouse neural stem cells (mNSCs). Methods: GO NPs were characterized with transmission electron microscopy (TEM), scanning electron micrography (SEM), atomic force microscopy (AFM) and Raman Spectra analysis. The cytotoxic effects of the GO NPs of different sizes on the mNSCs were determined using CCK-8 assay, Annexin V-APC/ 7-AAD staining and EdU staining assays. We investigated the biological and the mechanisms of GO NPs on cells using immunofluorescence analysis and quantitative real-time PCR (qPCR). Results: The average hydrodynamic sizes of the GO NPs were 417 nm, 663 nm, 1047 nm, and 4651 nm, with a thickness of approximately 22.5 nm, 17.7 nm, 22.4 nm, and 13.4 nm, respectively. GO NPs of all sizes showed low cytotoxicity at a concentration of 20 μg/mL on the mNSCs. Immunostaining demonstrated that treatment with GO NPs, especially the 663 nm ones, enhanced the self-renewal ability of mNSCs in the absence of EGF and bFGF. Under differentiation medium conditions that are free of mitogenic factors, all the GO NPs, particularly the 4651 nm ones, increased the expression level of Tuj1 and GFAP. With regards to the migration ability, we found that 417 nm GO-NP-treated mNSCs migrated over a longer distance than the control group obviously. In addition, higher expression of Rap1, Vinculin and Paxillin was observed in the GO NP-treated groups compared to the control group. mRNA-Sequence analysis and Western blotting results suggested that the 4651 nm GO NPs triggered positive neuronal differentiation through phosphorylation of ERK1/2 by the downregulating of TRPC2. Conclusion: GO NPs play an important role in the applications of inducing self-renewal and differentiation of mNSC, and are promising in the future for further studies.

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Influence of nanomaterials on stem cell differentiation: designing an appropriate nanobiointerface

Cited by 34 publications

References 89 publications

Trends in Tissue Regeneration: Bio-Nanomaterials

Trends in Tissue Regeneration: Bio-Nanomaterials

Novel Strategies for the Improvement of Stem Cells’ Transplantation in Degenerative Retinal Diseases

<p>Size-Dependent Effects of Suspended Graphene Oxide Nanoparticles on the Cellular Fate of Mouse Neural Stem Cells</p>

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