Influence of nanopatterns on endothelial cell adhesion: Enhanced cell retention under shear stress

Cooperative control of blood compatibility and re-endothelialization by immobilized heparin and substrate topography

“…Importantly, a recent study demonstrated that the elongation and alignment of ECs induced by micropatterns decreased EC immunogenic gene expression and function compared with cobblestone ECs, indicating that the elongated shape of ECs might be the key contributor to EC-mediated atheroprotection [39]. In addition, the groove pattern was demonstrated to enhance cell retention under shear flow conditions [40]. Our results show that the alignment and elongation of ECs were manipulated primarily by substrate topography regardless of surface chemistry.…”

Osteogenic differentiation of adipose derived stem cells on high and low aspect ratio micropatterns

“…These results indicated that EDC/NHS-crosslinked collagen-silk blend films with or without micropatterns did not have a negative effect on the viability of ADSC. Literature also indicates that collagen and silk fibroin are biocompatible materials as shown by high cell viability of human microvascular endothelial cells on EDC/NHS crosslinked collagen films [14] and feline fibroblasts (90% viability on Day 3) on biospin…”

“…100 micrometers) were shown to be ineffective in guiding and aligning the cells. We also showed earlier the improvement of mechanical properties of vascular smooth muscle cell seeded films [13] and human microvascular endothelial cell retention under the shear stress [14] , and the guidance of the cell alignment with the use of nanopatterns much lower in size than that of the cells (332.5 and 650 nm).…”

“…Also localization of focal adhesion proteins may have an influence on alignment [12] . It was shown that the guidance of the cell alignment with the use of nanopatterns much lower in size than that of the cells (332.5 and 650 nm) was possible and these topographies helped to improve the mechanical properties of cell seeded films [13] and human microvascular endothelial cell adhesion under shear stress [14] .…”

Nanotopography and Plasma Treatment: Redesigning the Surface for Vascular Graft Endothelialisation

“…When cultured on microgrooves, flow only detached 8% of cells compared with 42% on flat surfaces; in another study, nanopatterns enhanced adhesion from 37% to 91%. 32 Furthermore, presentation of topography and flow, respectively, has significant impact on EC alignment and migration, indicating a synergistic role between the biophysical cues. 33 Therefore, further work will focus further on surface optimisation, in particular on interactions with proteins (e.g.…”