Influence of natural aquatic humic substances on the bioavailability of benzo(a)pyrene to Atlantic Salmon

Johnsen, Ståle; Kukkonen, Jussi V. K.; Grande, Magne

doi:10.1016/0048-9697(89)90180-0

Cited by 17 publications

(4 citation statements)

References 20 publications

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“…Many studies have suggested that HoA fractions of DOM in soil and water bind organic contaminants and affect the uptake of contaminants by microorganisms and fishes (Haitzer et al . 1999; Johnsen et al . 1989; Kukkonen et al .…”

Section: Introductionmentioning

confidence: 99%

Characterization of hydrophobic acid fractions in water-soluble organic matter in Dystric Cambisol and in a stream in a small forested watershed: Seasonal and vertical variations in chemical properties

Asakawa

Mochizuki

Yanagi

et al. 2007

Soil Science and Plant Nutrition

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Chemical properties of hydrophobic acid (HoA) fractions in water-soluble organic matter in soil and water are concerned with its interactions with mineral soil surfaces and organic pollutants. In 2004 we examined the seasonal and vertical changes in chemical properties of the HoA fraction in a Cambisol profile and compared these properties with those in the HoA fraction of an adjacent stream (aquatic humic substances) in a temperate forested watershed using high performance size exclusion chromatography (HPSEC) and 1 H and 13 C nuclear magnetic resonance (NMR) spectroscopy. The HoA fractions from Oi, Oe/Oa, A and B horizon soils in summer had lower O-alkyl C proportions than those recorded in samples in other seasons. The proportions of aromatic C in HoA fractions from A and B horizons were highest in summer. These seasonal variations were less significant than variations with soil depth. O-alkyl C proportions in HoA fractions decreased with increasing soil depth from the Oi to the A horizon. The HoA fractions from the B horizon showed a higher alkyl C proportion than samples from other horizons in winter and spring. These changes with soil depth from the Oi to A horizons might result from selective utilization of carbohydrate carbon by microorganisms, whereas those in the B horizon may result from sorption to mineral surfaces. The HoA fractions in the stream were similar in relative molecular weight, distribution of each type of proton and carbon species in HoA fractions from the B horizon, whereas stream HoA fractions collected in summer would be derived from organic horizons. This indicated that vertical changes in the chemical properties of HoA fractions in soil and pathways of water to the stream would largely affect the chemical properties of HoA fractions in the stream.

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Section: Introductionmentioning

confidence: 99%

Characterization of hydrophobic acid fractions in water-soluble organic matter in Dystric Cambisol and in a stream in a small forested watershed: Seasonal and vertical variations in chemical properties

Asakawa

Mochizuki

Yanagi

et al. 2007

Soil Science and Plant Nutrition

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“…There is a complex relationship between sorption to DOM and the bioavailability of contaminants (41). Binding of hydrocarbons to HS decreases their bioavailability (e.g., [12][13][14][15][16][17]. Yet, HS also could amplify uptake of hydrocarbons by fish, by increasing the solubility of chemicals (e.g., [19][20] or by affecting gill membrane permeability (22)(23).…”

Section: Cyp1a Induction In Tambaqui By Hs and Crude Oilmentioning

confidence: 99%

“…Humic substances affect the bioavailability of organic contaminants through different mechanisms of binding and adsorption (). A number of studies have indicated decreased bioavailability of lipophilic pollutants to aquatic organisms in the presence of HS ( − ). In a few cases, however, HS have been reported to increase the solubility of petroleum hydrocarbons, possibly resulting in higher bioavailability to aquatic organisms ( − ).…”

Section: Introductionmentioning

confidence: 99%

Humic Substances and Crude Oil Induce Cytochrome P450 1A Expression in the Amazonian Fish Species Colossoma macropomum (Tambaqui)

Matsuo

Woodin

Reddy

et al. 2006

Environ. Sci. Technol.

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Cytochrome P450 1A (CYP1A) induction is used widely as a biomarker of exposure to pollutants, such as petroleum hydrocarbons, yet CYP1A inducibility has been characterized in few tropical fish. Using Western blot analysis, catalytic assay, and immunohistochemistry, we evaluated CYP1A induction in an Amazonian fish (tambaqui; Colossoma macropomum) acclimated to humic substances (HS) and acutely exposed to crude oil. HS are ubiquitous in Amazonian waters, and they are known to affect the bioavailability of pollutants. CYP1A activity was also measured in fish exposed for 10 days to a range of concentrations of HS from both natural and commercial sources. Crude oil induced CYP1A expression in tambaqui, as expected. Exposure to both HS and crude oil resulted in greater levels of CYP1A expression relative to that in fish exposed to petroleum alone. Interestingly, CYP1A induction was also observed in fish exposed to HS alone. Induction by HS was concentration-dependent, and activity was higher in fish exposed to HS from the commercial source than in fish exposed to the HS from the natural source. The use of CYP1A as a biomarker of exposure to pollutants such as petroleum hydrocarbons in fish living in environments rich in humic substances should be considered with caution given that HS themselves induce CYP1A expression. Our results suggest that there may be as yet unknown CYP1A inducing components (aryl hydrocarbon receptor agonists) in humic substances.

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“…[10][11][13][14]261 and are defined asThe terms BCFand ARS follow the uses of oth-where ARS = accumulation relative to sediment F, = concentration of toxicant in whole fish S, = concentration of toxicant in sediment (in tissue like units). BCF = FJW, where BCF = bioconcentration factor (at steady state) F, = concentration of toxicant in whole fish W, = total concentration of toxicant in water.…”

mentioning

confidence: 99%

Humic Acid Effects on Uptake of Hexachlorobenzene and Hexachlorobiphenyl by Sheepshead Minnows in Static Sediment/Water Systems

Lores

Patrick

Summers

1993

Environ Toxicol Chem

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The effect of humic acid on accumulation of hexachlorobenzene (HCB) and hexachlorobiphenyl (HCBP) by sheepshead minnows (Cyprinodon variegatus) from two separate experiments is presented. In the first experiment, static sedimentlwater exposure chambers were used to determine the effect of dissolved organic carbon (DOC), in the form of terrestrial humic acid (HA), on partitioning of HCB among sediment, water, and sheepshead minnows. Sediments from three geographically different locations were used to test the effect of added HA (0, 3, and 30 mg/L) on accumulation in fish of sediment-bound HCB. Total organic carbon levels in sediment and water and residues of HCB (I4C-labeled and unlabeled) in sediment, water, and whole-body tissue were measured. Fishlsediment ratios or accumulation relative to sediment (ARS) indicated that addition of HA did not reduce accumulation of sediment-bound toxicants. ARS ranged from 7.5 ? 4 without added HA to 9.3 k 4 with 30 mg/L added HA, but were not statistically different. In a second experiment using HCBP with 0 and 30 mg/L HA and sediment from one location, HCBP concentration in water averaged 0.29 pg/L in non-HA tanks and 0.91 pg/L in the HA tanks, but both ARS ratios averaged 5.4. In conclusion, we believe that these tests indicate that HA does not significantly alter bioavailability of toxicants that are in equilibrium with sediment and water.

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Influence of natural aquatic humic substances on the bioavailability of benzo(a)pyrene to Atlantic Salmon

Cited by 17 publications

References 20 publications

Characterization of hydrophobic acid fractions in water-soluble organic matter in Dystric Cambisol and in a stream in a small forested watershed: Seasonal and vertical variations in chemical properties

Characterization of hydrophobic acid fractions in water-soluble organic matter in Dystric Cambisol and in a stream in a small forested watershed: Seasonal and vertical variations in chemical properties

Humic Substances and Crude Oil Induce Cytochrome P450 1A Expression in the Amazonian Fish Species Colossoma macropomum (Tambaqui)

Humic Acid Effects on Uptake of Hexachlorobenzene and Hexachlorobiphenyl by Sheepshead Minnows in Static Sediment/Water Systems

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