INFLUENCE OF NITROGEN, ACETATE AND PROPIONATE ON HYDROGEN PRODUCTION FROM PINEAPPLE WASTE EXTRACT BY Rhodospirillum rubrum

Abstract

This research examined the influence of nitrogen, acetate and propionate on hydrogen production from pineapple waste extract by photosynthetic bacteria strain Rhodospirillum rubrum in batch culture. The fermentation conditions used in this study were continuous illumination fermentation (24 hours of light) and periodic illumination fermentation (alternate 12 hours of light and dark). Two levels of total nitrogen (3 mM-low level and 11 mM-high level) with various initial concentrations of acetate or propionate (5, 10 and 20 mM) were added into the production medium. Results indicated that levels of total nitrogen did not affect the production of hydrogen. Neither acetate nor propionate was used as carbon source by R. rubrum but glucose contained in pineapple waste extract was used. Periodic illuminated fermentation was more effective in producing hydrogen than continuous illuminated fermentation. The maximum hydrogen production potential (337 ml), specific hydrogen production rate (11 ml/l/h), specific hydrogen production potential (247.75 ml H 2 /g COD) and hydrogen yield (122 ml H 2 /g glucose consumed) occurred upon addition of high level of total nitrogen (11 mM) with 5 mM initial concentrations of acetate under periodic illumination and a working volume of 40 ml. Results indicated that pineapple waste extract could be effectively used as substrate for hydrogen production by R. rubrum without any carbon and nitrogen sources.

Bio-Hydrogen Production from Pineapple Waste Extract by Anaerobic Mixed Cultures

“…Pineapple waste is not considered attractive as an animal feed because of its high fiber content, high soluble carbohydrate and low protein content [4]. However, the pineapple waste extract, i.e., the juice obtained after the pineapple waste has been squeezed by a presser, mainly contains sugars and organic acids that can be utilized as the substrates in the production of hydrogen and ethanol [5][6][7][8]. Due to its composition, we attempted to use the pineapple waste extract in hydrogen production using anaerobic mixed cultures.…”

Biohydrogen production and bioprocess enhancement: A review

“…Biohydrogen production from the fermentation of renewable carbohydrate−rich and non−toxic raw materials (Kapdan and Kargi, 2006) is one promising alternative although the use of commercially produced food products, such as corn and sugar, is not yet economical (Benemann, 1996). Substrates used for biohydrogen production have ranged from simple sugars such as glucose (Li et al, 2008), sucrose (Antonopoulou et al, 2007), starch containing waste such as cassava wastewater (Sangyoka et al, 2007), dairy wastewater (Venkata Mohan et al, 2007a), sweet potato starch residue (Yokoi et al, 2001), sugarcane bagasse (Patra et al, 2008), cheese whey (Davila−Vazquez et al, 2009) and food waste (Ruknongsaeng et al, 2005). Others substrates for biohydrogen production were showed in Table 1.…”

Effect of Different Inoculum Combination on Biohydrogen Production from Melon Fruit Waste

“…Anaerobic fermentation is simpler because the process does not require light (Sivagurunathan et al, 2016;Hand and Shin, 2004) and it can apply to wide range of feedstocks (Nath and Das, 2004). Current research has studied various types of substrates for hydrogen production, such as glucose , sucrose (Choi and Ahn, 2015), galactose (Sivagurunathan et al, 2016), cassava starch (Tien et al, 2016), the starch residue of sweet potato (Yokoi et al, 2001), sugarcane bagasse (Pattra et al, 2008) and extracts of pineapple wastes (Ruknongsaeng et al, 2005). The availability, cost, carbohydrate content and biodegradability are the important criteria for the substrate selection (Kapdan and Kargi, 2006;Cai, et al, 2009).…”