2009
DOI: 10.1111/j.1365-2672.2009.04591.x
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Influence of nitrogen fertilization on tropical-grass silage assessed by ensiling process monitoring using chemical and microbial community analyses

Abstract: Aims:  Utilization of silage in livestock farming is expected to increase in developing countries in the tropical and subtropical parts of the world. The aim of this study was to investigate the influence of nitrogen fertilization on the chemical composition of herbage, ensiling process and silage quality, and to contribute to the improvement of tropical‐grass silage preparation. Methods and Results:  Guinea grass grown under two different nitrogen‐fertilizer application conditions [1·5 kg N a−1 (high‐N) and 0… Show more

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Cited by 24 publications
(28 citation statements)
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“…Keady & Mayne (1996) and Wilson (1969) observed a decrease in soluble carbohydrates and a higher buffering capacity with increasing doses of N applied to grasses. Reduction in the concentration of WSC with higher doses of N was also observed by Keady et al (2000) and Namihira et al (2009). The increased translocation of WSC for the growth of treated plants provides a possible explanation for this result.…”
Section: Resultssupporting
confidence: 60%
“…Keady & Mayne (1996) and Wilson (1969) observed a decrease in soluble carbohydrates and a higher buffering capacity with increasing doses of N applied to grasses. Reduction in the concentration of WSC with higher doses of N was also observed by Keady et al (2000) and Namihira et al (2009). The increased translocation of WSC for the growth of treated plants provides a possible explanation for this result.…”
Section: Resultssupporting
confidence: 60%
“…Ensilage under farm conditions is not a completely controlled process, because various factors can influence the ensiling process, such as plant species, method of ensiling, climate and DM content (Monteiro et al 1998;Namihira et al 2010;Nishino et al 2012). For the preparation of well-preserved silage, rapid development of the lactic acid fermentation is necessary to reduce the pH and inhibit the growth of inefficient and spoilage microorganisms such as clostridia, enterobacteria, yeast and moulds, which can cause degradation of plant material and/or production of undesirable metabolites (McDonald et al 1991).…”
Section: Introductionmentioning
confidence: 99%
“…Recent advancements in molecular tools have enabled us to elucidate the entire structure of the complex microbial community found in nature. One frequently used method is denaturing gradient gel electrophoresis (DGGE) analysis, which can demonstrate the dynamics of each microbial constituent in microcosms (Namihira et al 2010). DGGE analysis has been shown to help understand how LAB and non-LAB species function in the ensiling process for various crop species (Nishino et al 2012).…”
Section: Introductionmentioning
confidence: 99%
“…In recent years, the demand for dairy products has increased in many developing countries in the tropical and subtropical regions of Asia and Africa; however, production of the silage necessary for dairy farming has been hindered in these regions because the ensiling process is dependent on local environmental conditions (Namihira et al 2010). For example, virtually all of the many available silage starters (Holzer et al 2003; Yan et al 2008) are hampered by high temperatures and humidity (Ohmomo et al 2002; Mulrooney and Kung 2008).…”
Section: Introductionmentioning
confidence: 99%
“…Some PCR amplification methods for the 16S-23S rRNA, recA and dnaK genes have been used to identify L. plantarum -related species (Torriani et al 2001; Huang et al 2010; Ouoba et al 2010), but there is no generally accepted or systematic approach to identification of silage-making lactobacilli. In particular, there have been few studies characterizing LAB strains grown in silage produced in tropical or subtropical regions (Namihira et al 2010). …”
Section: Introductionmentioning
confidence: 99%