Influence of Nitrogen Supply of Potato Plantlets on In Vitro Tuberization Pattern under Inductive and Non-inductive Conditions

Dobránszki, Judit; Tábori, K. M.

doi:10.1007/s11540-010-9156-z

Cited by 8 publications

(3 citation statements)

References 9 publications

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“…It has been known that various environmental and endogenous factors influence tuberization (Nookaraju et al ). A large number of studies have shown that short photoperiod, high light intensity, low temperature, low nitrogen levels and high sucrose levels can promote tuberization (Dobránzki et al , Tadesse et al , EL‐Sawy et al , Dobránszki and Tábori ). Phytohormones also play a crucial role in regulating the morphological events of tuberization (Aksenova et al , Roumeliotis et al ).…”

Section: Introductionmentioning

confidence: 99%

Comparative proteomics illustrates the molecular mechanism of potato (Solanum tuberosum L.) tuberization inhibited by exogenous gibberellins in vitro

Cheng

Wang

Liu

et al. 2018

Physiologia Plantarum

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Among the multiple environmental signals and hormonal factors regulating potato tuberization, gibberellins (GAs) are important components of the signaling pathways in these processes. To understand the GAs-signaling response mechanism of potato tuberization, a comparative proteomics approach was applied to analyze proteome change of potato tuberization in vitro subjected to a range of exogenous GA treatments (0, 0.01, 0.1 and 1.0 μM) using two-dimensional gel electrophoresis. Quantitative image analyses showed that a total of 37 protein spots have their abundance significantly altered more than 2-fold. Among these proteins, 13 proteins were up-regulated, 13 proteins were down-regulated, one protein was absent and 10 proteins were induced after treatment by exogenous GA . The MALDI-TOF/TOF MS analyses led to the identification of differentially abundant proteins that are mainly involved in bioenergy and metabolism, storage, signaling, cell defense and rescue, transcription, chaperones, transport. Furthermore, the comparative analysis of GA -responsive proteome allowed for general elucidation of underlying molecular mechanisms of potato tuberization inhibited by exogenous GA . Most of these cellular processes were not conducive to the transition from stolon elongation to tuber formation, including a blockage of starch and storage protein accumulation, the accelerated carbohydrate catabolism, a blockage of JA biosynthesis but an elevated endogenous GAs level, the amplification of GA signal transduction by other signaling pathways, and the regulation of cellular RNA metabolism for controlling tuberization. Our results firstly integrated physiology and proteome data to provide new insights into GA -signaling response mechanisms of potato tuberization in vitro.

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Section: Introductionmentioning

confidence: 99%

Comparative proteomics illustrates the molecular mechanism of potato (Solanum tuberosum L.) tuberization inhibited by exogenous gibberellins in vitro

Cheng

Wang

Liu

et al. 2018

Physiologia Plantarum

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“…This activity was influenced by the nitrogen content in the growth medium. High concentration of nitrogen was favorable to yielding larger microtuber (Dobranszki et al, 2008). The distal part could be played the role of front-door for almost nutriments in the tuber and the two other parts an accumulation zone.…”

Section: Discussionmentioning

confidence: 97%

Partial Study of Yam Tuber (<em>Dioscorea</em> spp.) Parts during the Growth Period

Degbeu¹,

Ndri²,

Tetchi³

et al. 2013

AJFST

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Growing gradient into longitudinal axis of yam tuber was study through starch properties and nutritional composition of tuber parts (proximal, median and distal) during the tuberization. Two varieties of the complex D. cayenensis-rotundata (kangba and kponan) were used. Clarity, swelling and solubility, flow behavior and syneresis of starch and protein content, alcohol-soluble sugars of the flour were studies. The ash assessed by microanalysis. Starch clarity of tuber parts increased during the growth period. The proximal (56±2.06%) and median (54.5±1.09%) parts exhibited high clarity than the distal (48.2±2.56%) one. At earlier stage of tuberization, the viscosity ratio of tuber parts (var. Kponan) was weak. This indicates the possibility to use it as thickening agent in shearing sauce. Water loss decreased during the growth period for the three tuber parts. It was the same behavior for the swelling power. Concerning the nutritional composition of the flour, amount of protein did not influenced by the degree of tuber maturity. Alcohol-soluble sugars were higher at earlier stage of tuberization than at maturity of the tuber. Mineral content was appreciable at maturity than at beginning of the tuberization. The extent mineral according to their amount was: K, P, Mg and S. Starch properties such as clarity, syneresis and swelling were improved at maturity of the tuber. It was more for the proximal and median parts than the distal one. Yam tuber content a appreciable amount of protein and mineral at maturity.

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“…Micro tubers offer advantages of small space accommodation, ease of transport and storage in addition to solving the problems of transplanting of plantlets [27]. Micro tubers are also useful in other applications, including germplasm storage and exchange, or as experimental research tools in the areas of plant metabolism, germplasm selection and evaluation, transformation, somatic hybridization or molecular farming, and for in vitro selection of agronomical important characteristics, such as maturity and abiotic stress tolerance [28]. It was indicated that micro tuber induction of potato was highly dependent on sucrose and genotype interaction [29].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of BAP Effects on Plantlets Micro Tuberization of Five Potato Cultivars

Saljooghianpour

2017

JALSI

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The effects of BAP on the micro tuberization were evaluated using five commercial cultivars of potato. Potato tubers were planted in the greenhouse and young shoots were cut and transferred to Tissue Culture Laboratory of Agricultural Research Institute. Apical and axillary bud explants were cultured on liquid MS medium containing 2 mgl-1 GA 3. The biological samples were subculture every four weeks on the same medium for plantlet production and propagation. Then, for plantlets micro tuberization, plantlets were cultured on liquid MS medium with different concentrations of BAP (0, 5, 10, 15 and 20 mgl-1). After eight weeks characteristics, such as the number of micro tubers, micro tuber fresh weight, micro tuber dry weight, the mean of micro tuber diameter, the mean of eyes number, the mean of small micro tuber diameter, the mean of large micro tuber diameter, the mean of eyes number on the small micro tubers and the mean of eyes number on the large micro tubers were recorded. Morphological parameters of micro tubers varied significantly among cultivars and BAP concentrations at P < 0.05 or P < 0.01. Results indicated that cultivars produced significantly higher number of micro tubers in the presence of BAP. For the tested cultivars, micro tuberization was maximum in liquid MS medium containing 5 mgl-1 BAP and it was lowest in 0 mgl-1 BAP.

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Influence of Nitrogen Supply of Potato Plantlets on In Vitro Tuberization Pattern under Inductive and Non-inductive Conditions

Cited by 8 publications

References 9 publications

Comparative proteomics illustrates the molecular mechanism of potato (Solanum tuberosum L.) tuberization inhibited by exogenous gibberellins in vitro

Comparative proteomics illustrates the molecular mechanism of potato (Solanum tuberosum L.) tuberization inhibited by exogenous gibberellins in vitro

Partial Study of Yam Tuber (<em>Dioscorea</em> spp.) Parts during the Growth Period

Evaluation of BAP Effects on Plantlets Micro Tuberization of Five Potato Cultivars

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