Influence of nutritive factors on C50 carotenoids production by Haloferax mediterranei ATCC 33500 with two-stage cultivation

Fang, Chaojun; Ku, Kuo-Lung; Lee, Min-Hsiung; Su, Nan‐Wei

doi:10.1016/j.biortech.2010.03.044

Cited by 92 publications

(68 citation statements)

References 14 publications

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“…The C 50 carotenoid BR is known to accumulate in several extremely halophilic archaea (15)(16)(17)(18)(19), but the identity of the responsible biosynthetic pathway remains unclear. In this study, we have identified three main genes encoding enzymes responsible for the biosynthesis of BR from lycopene for the first time.…”

Section: Discussionmentioning

confidence: 99%

“…BR of Haloferax volcanii has been isolated and identified by chromatographic (thin-layer chromatography [TLC] and high-performance liquid chromatography [HPLC]), spectroscopic (mass spectrometry [MS], nuclear magnetic resonance [NMR] analysis, and circular dichroism [CD] spectroscopy), and chemical (silylation and methylation) methods (16). Haloferax mediterranei can also accumulate BR, and a two-stage culture method has been applied to increase production of C 50 carotenoids in the laboratory (17). In addition to studies on the antioxidant properties of BR (18), the regulating mechanism of BR biosynthesis in Hb.…”

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confidence: 99%

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Complete Biosynthetic Pathway of the C₅₀Carotenoid Bacterioruberin from Lycopene in the Extremely Halophilic Archaeon Haloarcula japonica

Yang¹,

Yatsunami²,

Ando³

et al. 2015

J. Bacteriol.

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Haloarcula japonica, an extremely halophilic archaeon that requires high concentrations of NaCl for growth, accumulates the C 50 carotenoid bacterioruberin (BR). By homology analysis, a gene cluster, including c0507, c0506, and c0505, was found and predicted to be involved in the synthesis of bacterioruberin. To elucidate the function of the encoded enzymes, we constructed Ha. japonica mutants of these genes and analyzed carotenoids produced by the mutants. Our research showed that c0507, c0506, and c0505 encoded a carotenoid 3,4-desaturase (CrtD), a bifunctional lycopene elongase and 1,2-hydratase (LyeJ), and a C 50 carotenoid 2؆,3؆-hydratase (CruF), respectively. The above three carotenoid biosynthetic enzymes catalyze the reactions that convert lycopene to bacterioruberin in Ha. japonica. This is the first identification of functional CrtD and CruF in archaea and elucidation of the complete biosynthetic pathway of bacterioruberin from lycopene. IMPORTANCEHaloarcula japonica, an extremely halophilic archaeon, accumulates the C 50 carotenoid bacterioruberin (BR). In this study, we have identified three BR biosynthetic enzymes and have elucidated their functions. Among them, two enzymes were found in an archaeon for the first time. Our results revealed the biosynthetic pathway responsible for production of BR in Ha. japonica and provide a basis for investigating carotenoid biosynthetic pathways in other extremely halophilic archaea. Elucidation of the carotenoid biosynthetic pathway in Ha. japonica may also prove useful for producing the C 50 carotenoid BR efficiently by employing genetically modified haloarchaeal strains.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Complete Biosynthetic Pathway of the C₅₀Carotenoid Bacterioruberin from Lycopene in the Extremely Halophilic Archaeon Haloarcula japonica

Yang¹,

Yatsunami²,

Ando³

et al. 2015

J. Bacteriol.

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“…-Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) as carbon and energy reserve 8,9,10,11 -EPS as protecting biofilms 12,13 -C50-(bisanhydrobacterioruberin, monoanhydrobacterioruberin and bacterioruberin) and C45 (2-isopentenyl-3,4-dehydrorhodopin) carotenoide Study on the Production and Re-use of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and Extracellular Polysaccharide by the Archaeon Haloferax mediterranei Strain DSM 1411 pigments for fluidity of the membrane, adaptation to salinity and temperature, and as scavengers of free radicals 14,15 , and -Bacteriocins (in this case: halocins) against microbial attack 1,4 . Production of these valued compounds was described starting from carbon sources like glucose 16 , hydrolyzed lactose from dairy waste 9,10 , starchy residues 17,18 , vinasse 19 , rice-based ethanol stillage 20 , or glycerol from biodiesel production 21 .…”

Section: Biotechnological Potential Of the Strain Haloferax Mediterraneimentioning

confidence: 99%

Study on the Production and Re-use of Poly(3-hydroxybutyrate-co-3- hydroxyvalerate) and Extracellular Polysaccharide by the Archaeon Haloferax mediterranei Strain DSM 1411

Koller

Chiellini

Braunegg

2015

Chem.Biochem.Eng.Q.

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The halobacterium Haloferax mediterranei was used to study the production of two types of biopolymers: The biopolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was accumulated as intracellular granules, whereas an extracelluar polysaccharide was excreted in parallel to biopolyester synthesis.After production, microbial re-use and degradation of these polymers under different conditions were investigated to assess the requirements for handling the product-rich fermentation broth prior to the downstream processing for product recovery. Degradation kinetics of the polymers and the impact of different storage conditions on molar mass of PHBV were studied.It turned out that the biotechnological fermentation process can be run without any sterility precautions. No major product losses were observed without pasteurization of fermentation broth after the stop of fermentation. In addition, neither PHBV nor EPS are re-utilized by the cells for biomass formation even if the culture is maintained under conditions of carbon starvation for an extended time.

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“…However, this general pattern has some exceptions, for example Haloquadratum walsbyi: Archaea -New Biocatalysts, Novel Pharmaceuticals and Various Biotechnological Applicationscells grown under osmotic stress did not experience changes in terms of either membrane lipid content or carotenoid production [38]. Composition of the total carotenoid fraction in haloarchaeal cells can also vary based on the nutritive factors within the culture media [39], the light intensity, oxygen tension, NaCl concentration [39,40], and other physical-chemical parameters such as the pH value of the culture media.…”

Section: Carotenoids Produced By Haloarchaeamentioning

confidence: 99%

Biocompounds from Haloarchaea and Their Uses in Biotechnology

Torregrosa-Crespo¹,

Galiana²,

MaríaMartínez-Espinosa³

2017

Archaea - New Biocatalysts, Novel Pharmaceuticals and Various Biotechnological Applications

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New advances in the understanding of haloarchaea physiology, metabolism, biochemistry, and molecular biology show that these kinds of microorganisms produce several compounds in response to the extreme conditions of their ecosystems. Thus, the complete metabolic and genetic machineries are fully adapted to nutrient starvation, high sun radiation, and high ionic strength. Due to these adaptations, some of the primary and secondary metabolites produced by haloarchaea are of high interest in terms of potential biotechnological uses. The principal goal of the chapter is to present a review about the main characteristics of these biocompounds and their potential uses in biomedicine, pharmacy, and industry.

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Influence of nutritive factors on C50 carotenoids production by Haloferax mediterranei ATCC 33500 with two-stage cultivation

Cited by 92 publications

References 14 publications

Complete Biosynthetic Pathway of the C₅₀Carotenoid Bacterioruberin from Lycopene in the Extremely Halophilic Archaeon Haloarcula japonica

Complete Biosynthetic Pathway of the C₅₀Carotenoid Bacterioruberin from Lycopene in the Extremely Halophilic Archaeon Haloarcula japonica

Study on the Production and Re-use of Poly(3-hydroxybutyrate-co-3- hydroxyvalerate) and Extracellular Polysaccharide by the Archaeon Haloferax mediterranei Strain DSM 1411

Biocompounds from Haloarchaea and Their Uses in Biotechnology

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Influence of nutritive factors on C50 carotenoids production by Haloferax mediterranei ATCC 33500 with two-stage cultivation

Cited by 92 publications

References 14 publications

Complete Biosynthetic Pathway of the C50Carotenoid Bacterioruberin from Lycopene in the Extremely Halophilic Archaeon Haloarcula japonica

Complete Biosynthetic Pathway of the C50Carotenoid Bacterioruberin from Lycopene in the Extremely Halophilic Archaeon Haloarcula japonica

Study on the Production and Re-use of Poly(3-hydroxybutyrate-co-3- hydroxyvalerate) and Extracellular Polysaccharide by the Archaeon Haloferax mediterranei Strain DSM 1411

Biocompounds from Haloarchaea and Their Uses in Biotechnology

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Complete Biosynthetic Pathway of the C₅₀Carotenoid Bacterioruberin from Lycopene in the Extremely Halophilic Archaeon Haloarcula japonica

Complete Biosynthetic Pathway of the C₅₀Carotenoid Bacterioruberin from Lycopene in the Extremely Halophilic Archaeon Haloarcula japonica