Influence of obese phenotype on metabolic profile, inflammatory mediators and stemness of hADSC in adipose tissue

Rawal, Komal; Patel, Tushar; Purohit, Kishan M.; Israni, Kashish; Kataria, Vyakhaya; Bhatt, Hiren; Gupta, Sarita

doi:10.1016/j.clnu.2020.02.032

Cited by 12 publications

(18 citation statements)

References 10 publications

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“…This interesting phenomenon has attracted the attention of this research group. Reading previous research literature found that there have been reports verifying abnormal osteogenic differentiation or poor fracture healing caused by obesity 22,23 . In this study, we also verified this phenomenon through in vivo and in vitro experiments.…”

Section: Discussionsupporting

confidence: 80%

“…Reading previous research literature found that there have been reports verifying abnormal osteogenic differentiation or poor fracture healing caused by obesity. 22,23 In this study, we also verified this phenomenon through in vivo and in vitro experiments. Exosomes have been proven to affect cell functions as an important link in cell-to-cell communication.…”

Section: Discussionsupporting

confidence: 66%

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Obesity regulates miR‐467/HoxA10 axis on osteogenic differentiation and fracture healing by BMSC‐derived exosome LncRNA H19

Wang

Chen

Zhao

et al. 2021

J Cellular Molecular Medi

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This study explored the therapeutic effect of bone marrow mesenchymal stem cell‐derived exosomes on the treatment of obesity‐induced fracture healing. Quantitative real‐time PCR was used to detect the expression of lncRNA H19, miR‐467 and Hoxa10 and combined with WB detection to detect osteogenic markers (RUNX2, OPN, OCN). Determine whether exosomes have entered BMSCs by immunofluorescence staining. Alkaline phosphatase (ALP) and alizarin red staining (ARS) staining were used to detect ALP activity and calcium deposition. We found that high‐fat treatment can inhibit the secretion of BMSCs‐derived exosomes and affect the expression of H19 carried by them. In vivo and in vitro experiments show that high‐fat or obesity factors can inhibit the expression of osteogenic markers and reduce the staining activity of ALP and ARS. The treatment of exosomes from normal sources can reverse the phenomenon of osteogenic differentiation and abnormal fracture healing. Further bioinformatics analysis found that miR‐467 as a regulatory molecule of lncRNA H19 and Hoxa10, and we verified the targeting relationship of the three through dual luciferase report experiments. Further, we found similar phenomena in ALP and ARS staining. Bone marrow mesenchymal stem cell‐derived exosomes improve fracture healing caused by obesity.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 66%

Obesity regulates miR‐467/HoxA10 axis on osteogenic differentiation and fracture healing by BMSC‐derived exosome LncRNA H19

Wang

Chen

Zhao

et al. 2021

J Cellular Molecular Medi

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“…Leptin is assumed to exert pleiotropic effects, affecting numerous metabolic paths [77]. Early studies of leptin levels and expression in human organs have shown that serum leptin levels increase along with increased body fat mass, which supports the hypothesis that white adipose tissue adipocytes are a rich source of this hormone [14,38]. Moreover, leptin has been shown to enhance insulin sensitivity in peripheral tissues and increase glucose uptake and oxidation in skeletal muscles [60].…”

Section: Obesity Adipokines and Chronic Complicationsmentioning

confidence: 94%

“…The data presented by authors from all over the world are alarming [9][10][11][12][13]. Authors have shown that excess body weight impacts the dynamic increase in the incidence of hypertension, type 2 diabetes (T2DM) and ischaemic heart disease, not only in adults but also in adolescents [14][15][16][17][18]. The WHO estimates that there are more than 1.6 billion people living all over the world with BMI > 25 kg/m 2 , including 522 million subjects with BMI beyond the obesity threshold >30/kg/m 2 [1].…”

Section: Epidemiology Of Obesitymentioning

confidence: 99%

Adipokines and Obesity. Potential Link to Metabolic Disorders and Chronic Complications

Zorena

Jachimowicz-Duda²,

Ślęzak

et al. 2020

IJMS

279

196

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The World Health Organization (WHO) has recognized obesity as one of the top ten threats to human health. It is estimated that the number of obese and overweight people worldwide exceeds the number of those who are undernourished. Obesity is not only a state of abnormally increased adipose tissue in the body, but also of increased release of biologically active adipokines. Adipokines released into the circulating blood, due to their specific receptors on the surface of target cells, act as classic hormones affecting the metabolism of tissues and organs. What is more, adipokines and cytokines may decrease the insulin sensitivity of tissues and induce inflammation and development of chronic complications. Certainly, it can be stated that in an era of a global obesity pandemic, adipokines may gain more and more importance as regards their use in the diagnostic evaluation and treatment of diseases. An extensive search for materials on the role of white, brown and perivascular fatty tissue and obesity-related metabolic and chronic complications was conducted online using PubMed, the Cochrane database and Embase.

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“…Similar results were obtained when obese diabetic subjects from India and nonobese control served as donors of hADSCs so as to evaluate the on ASDC functions. Proliferation was reduced, expression of pluripotency genes was altered as well as diminishing osteogenesis (Rawal et al 2020).…”

Section: Obesitymentioning

confidence: 99%

A Multilevel Approach to the Causes of Genetic Instability in Stem Cells

González

2022

Handbook of Stem Cell Therapy

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This chapter addresses the genetic instability in stem cells, a central feature that is an important determinant for the safety and effectiveness of cell-based therapies. First, DNA damage response mechanism and the gene network that regulates the DNA integrity homeostasis are revisited, focusing on relationship between genetic integrity and stemness maintenance. Also, several factors have been included that influence genetic instability in vivo, i.e., ROS generation and inflammation, and in vitro regarding cell isolation, culture conditions, i.e., oxygen levels and the use of feeder layers and different carriers, splitting procedures, passage number, etc. Telomere shortening, replicative senescence aneuploidy, and the senescence-associated secretory phenotype are different processes involved in deterioration of stem cells abilities for homing, reparability, and paracrine modulation. Moreover, less effective DNA repair upon senescence increases the propensity of developing tumors for stem cells that is one the main concerns related to genetic instability in stem cells. Nuclear organization and their determinants linked to chromosome aberrations and aneuploidy in stem cells and those epigenetic changes affecting stability are addressed. Differences between adipose, embryonic, and induced pluripotent stem cells are analyzed regarding to their ontogeny, changes in culture, and variation in proliferative capacity and stemness. The effect of reprogramming methods in genetic instability and variation in mutagenicity according to genes utilized for pluripotency induction, i.e., Yamanaka's factors and others, is discussed. Donor-related factors, i.e., age, smoking, and alcohol consumption, comorbidities, i.e., obesity, insulin resistance, diabetes, are mentioned for wide evaluation of genetic instability. The next years must witness consensus protocols that will contribute to control the effects of factors that alter stem cell genetic stability to increase the security and applicability of cell-based therapy.

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Influence of obese phenotype on metabolic profile, inflammatory mediators and stemness of hADSC in adipose tissue

Cited by 12 publications

References 10 publications

Obesity regulates miR‐467/HoxA10 axis on osteogenic differentiation and fracture healing by BMSC‐derived exosome LncRNA H19

Obesity regulates miR‐467/HoxA10 axis on osteogenic differentiation and fracture healing by BMSC‐derived exosome LncRNA H19

Adipokines and Obesity. Potential Link to Metabolic Disorders and Chronic Complications

A Multilevel Approach to the Causes of Genetic Instability in Stem Cells

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