Influence of omega-3 fatty acids on bovine luteal cell plasma membrane dynamics

Abstract

Fish oil is a rich source of omega-3 fatty acids which disrupt lipid microdomain structure and affect mobility of the prostaglandin F2α (FP) receptor in bovine luteal cells. The objectives of this study were to determine the effects of individual omega-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on 1) membrane fatty acid composition, 2) lipid microdomain structure, and 3) lateral mobility of the FP receptor in bovine luteal cells. Ovaries were collected from a local abattoir (n = 5/experiment). The corpus luteum was resected and enzymatically digested using collagenase to generate a mixed luteal cell population. In all experiments, luteal cells were treated with 0, 1, 10 or 100 μM EPA or DHA for 72 h to allow incorporation of fatty acids into membrane lipids. Results from experiment 1 show that culturing luteal cells in the presence of EPA or DHA increased these luteal fatty acids. In experiment 2, both EPA and DHA increased spatial distribution of lipid microdomains in a dose-dependent manner. Single particle tracking results from experiment 3 show that increasing both EPA and DHA concentrations increased micro- and macro-diffusion coefficients, increased domain size, and decreased residence time of FP receptors. Collectively, results from this study demonstrate similar effects of EPA and DHA on lipid microdomain structure and lateral mobility of FP receptors in cultured bovine luteal cells. Moreover, only 10 μM of either fatty acid was needed to mimic the effects of fish oil.

Trafficking of cholesterol from lipid droplets to mitochondria in bovine luteal cells: Acute control of progesterone synthesis

“…To determine the effects of LH on the phosphorylation of HSL z‐stacked mages were converted to maximum intensity projections and processed utilizing Image J (National Institutes of Health) analysis software. Mean fluorescence intensity was determined as previously described 52‐54 . To determine the effects of LH on the colocalization of HSL with lipid droplets and TopFluor Cholesterol with mitochondria the JACoP plug‐in was used in Image J software to determine the Manders’ overlap coefficient for each image as previously described 55 and transformed into percent colocalization by multiplying Manders’ overlap coefficient by 100 for all colocalization experiments.…”

“…Mean fluorescence intensity was determined as previously described. [52][53][54] To determine the effects of LH on the colocalization of HSL with lipid droplets and TopFluor Cholesterol with mitochondria the JACoP plug-in was used in Image J software to determine the Manders' overlap coefficient for each image as previously described 55 and transformed into percent colocalization by multiplying Manders' overlap coefficient by 100 for all colocalization experiments.…”

Yes-associated protein (YAP) is required in maintaining normal ovarian follicle development and function

“…Cells were then converted to maximum intensity projections and processed utilizing ImageJ (National Institutes of Health) analysis software. Corrected total cell fluorescence intensity was determined as previously described [20-22] for the nuclear and cytoplasmic fractions.…”

Inflammation and angiogenesis in the corpus luteum

“…The corpus luteum (CL) is a transient endocrine structure that secrets progesterone (P4) to establish and maintain intrauterine pregnancy. [1][2][3][4][5] There are two kinds of steroidogenic cells depending on their origin: theca-lutein (small luteal cells) and granulosa-lutein (large luteal cells) cells. 6 In addition to steroidogenic cells, the CL contains endothelial cells (EC), pericytes, fibroblasts and immune cells.…”