Influence of operating conditions on the persistence of E. coli, enterococci, Clostridium perfringens and Clostridioides difficile in semi-continuous mesophilic anaerobic reactors

Derongs, Lorine; Druilhe, Céline; Caroline, Le Maréchal; Barbut, Frédéric; Heurtevent, Lorette; Buffet, Julie; Martin, Laure; Ziebal, Christine; Poëzévara, Typhaine; Rouxel, Sandra; Houard, Emmanuelle; Rabab, Syed Zaidi; Couturier, Jeanne; Pourcher, Anne-Marie

doi:10.1016/j.wasman.2021.08.003

Cited by 11 publications

(3 citation statements)

References 61 publications

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“…There were no significant decreases in the Enterococcus numbers throughout the 27 d incubation period. Enterococci are very resistant to environmental stress and treatment [ 11 , 37 ]. The findings from this study agrees with the work of Arias et al [ 26 ], who observed no clear trend in Enterococcus inactivation after 75 d of batch mesophilic AD, as well as Do et al [ 38 ], who also reported the resistance of Enterococcus during a 90 d batch AD of pig slurry.…”

Section: Discussionmentioning

confidence: 99%

“…Although it has been reported that AD reduces or eliminates human pathogens and antibiotic-resistant genes present in food wastes and other organic wastes [ 2 , 7 , 8 , 9 ], safety concerns remain with the recycling of digestate through its application to soil. This is because certain pathogenic species may persist in the digestate despite AD treatment [ 10 , 11 ]. This has raised questions on the efficacy of the AD process in sanitizing food wastes which could contain high concentrations of potentially pathogenic enteric species such as Salmonella enterica , Escherichia coli , Enterococcus faecalis , and Shigella spp.…”

Section: Introductionmentioning

confidence: 99%

“…Although it has been reported that AD reduces or eliminates human pathogens and antibiotic-resistant genes present in food wastes and other organic wastes [2,[7][8][9], safety concerns remain with the recycling of digestate through its application to soil. This is because certain pathogenic species may persist in the digestate despite AD treatment [10,11].…”

Section: Introductionmentioning

confidence: 99%

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Effect of the Inoculum-to-Substrate Ratio on Putative Pathogens and Microbial Kinetics during the Batch Anaerobic Digestion of Simulated Food Waste

Otite,

Gandhi,

Agyabeng Fofie

et al. 2024

Microorganisms

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The effects of the inoculum (anaerobic digestion effluent) to substrate (simulated food waste) ratio (ISR) 4.00 to 0.25 on putative pathogens and microbial kinetics during batch mesophilic anaerobic digestion were investigated. Red fluorescent protein labelled (RFPAKN132) Escherichia coli JM105 was introduced as a marker species, and together with the indigenous Clostridium sp., Enterococcus sp., Escherichia coli, and total coliforms were used to monitor pathogen death kinetics. Quantitative polymerase chain reaction was also used to estimate the bacterial, fungal, and methanogenic gene copies. All the ISRs eliminated E. coli and other coliforms (4 log10 CFU/mL), but ISR 0.25 achieved this within the shortest time (≤2 days), while ISR 1.00 initially supported pathogen proliferation. Up to 1.5 log10 CFU/mL of Clostridium was reduced by acidogenic conditions (ISR 0.25 and 0.50), while Enterococcus species were resistant to the digestion conditions. Fungal DNA was reduced (≥5 log10 copies/mL) and was undetectable in ISRs 4.00, 2.00, and 0.50 at the end of the incubation period. This study has demonstrated that ISR influenced the pH of the digesters during batch mesophilic anaerobic digestion, and that acidic and alkaline conditions achieved by the lower (0.50 and 0.25) and higher (4.00 and 2.00) ISRs, respectively, were critical to the sanitisation of waste.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of the Inoculum-to-Substrate Ratio on Putative Pathogens and Microbial Kinetics during the Batch Anaerobic Digestion of Simulated Food Waste

Otite,

Gandhi,

Agyabeng Fofie

et al. 2024

Microorganisms

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Integrating Anaerobic Digestion With Struvite Production for Enhanced Nutrient Recovery, Pathogen Reduction, and Circularity in Manure Management

Nagarajan,

Goyette,

Raghavan

et al. 2024

J of Sust Agri & Env

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IntroductionAnaerobic digestion (AD) is essential for manure management, generating biogas and nutrient‐rich digestate for organic fertilizer. However, improper digestate use can pose environmental risks. Recovering struvite, a magnesium ammonium phosphate (MAP) compound, from digestate provides a sustainable, controlled‐release fertilizer, supporting a circular economy in agriculture.Materials and MethodsThe study employed a two‐stage (liquid–solid) AD process using poultry, dairy, and swine manures, along with wasted corn silage. Digestates were sampled for physicochemical and biogas quality analyses, with feedstocks categorized into D1 and D2, and a composite (D3) formed for struvite characterization. Microbial populations were enumerated on selective media, and struvite mineral content was analysed via argon plasma emission spectrometry.ResultsThe digesters processing feedstock mixtures D1 and D2 achieved specific methane yields of 1.26 L/g CODs fed and 1.49 L/g CODs fed, with cumulative biogas production of 374 and 369 L, respectively, over four 77‐day cycles. The two‐stage AD process significantly reduced antibiotic‐resistant, Enterobacteriaceae and Enterococcus spp. Total ammoniacal nitrogen (TAN) recovery rates were high at 98%–99%, with a consistent struvite crystal mass of 0.67 g/10 mL, indicating the efficiency of this integrated process. The agronomic value of struvite was determined, indicating its potential utility as a fertilizer, and scanning electron microscopy analysis revealed diverse crystal structures, warranting further investigation into their implications for usage and storage.ConclusionThe results suggests that the two‐stage AD process efficiently transforms organic waste into high‐quality biogas, reduces antibiotic‐resistant bacteria, and facilitates nutrient recovery through struvite precipitation. This approach supports co‐digestion of multi‐substrates and promotes circular economy principles, with potassium or sodium phosphate enhancing struvite recovery for sustainable agriculture.

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Semi-solid anaerobic co-digestion of source-separated fecal slag and food waste: focusing on methane production, ecological risk assessment, and quality evaluation as fertilizer

Yusuf

Pan

Cai

et al. 2022

Environ Sci Pollut Res

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Influence of operating conditions on the persistence of E. coli, enterococci, Clostridium perfringens and Clostridioides difficile in semi-continuous mesophilic anaerobic reactors

Cited by 11 publications

References 61 publications

Effect of the Inoculum-to-Substrate Ratio on Putative Pathogens and Microbial Kinetics during the Batch Anaerobic Digestion of Simulated Food Waste

Effect of the Inoculum-to-Substrate Ratio on Putative Pathogens and Microbial Kinetics during the Batch Anaerobic Digestion of Simulated Food Waste

Integrating Anaerobic Digestion With Struvite Production for Enhanced Nutrient Recovery, Pathogen Reduction, and Circularity in Manure Management

Semi-solid anaerobic co-digestion of source-separated fecal slag and food waste: focusing on methane production, ecological risk assessment, and quality evaluation as fertilizer

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