Influence of operating parameters on the suture retention test for scaffolds in ophthalmology

Küng, Florian; Schubert, Dirk W.; Stafiej, Piotr; Kruse, Friedrich E.; Fuchsluger, Thomas Armin

doi:10.1016/j.msec.2017.02.177

Cited by 8 publications

(9 citation statements)

References 15 publications

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“…To quantify the suitability of electrospun nanofibrous scaffolds for the application as wound dressing, suture pullout is of crucial importance. Kueng et al [ 24 , 28 ] established a suture retention test (SRT) to characterize the ability of biomaterials as corneal wound dressings. Briefly, the SRT describes the maximum force F max necessary for suture pullout.…”

Section: Methodsmentioning

confidence: 99%

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Electrospun PCL Scaffolds as Drug Carrier for Corneal Wound Dressing Using Layer-by-Layer Coating of Hyaluronic Acid and Heparin

Himmler

Schubert²,

Dähne

et al. 2022

IJMS

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Due to its ability to reduce scarring and inflammation, human amniotic membrane is a widely used graft for wound dressings after corneal surgery. To overcome donor dependency and biological variances in the donor tissue, artificial nanofibrous grafts acting as drug carrier systems are promising substitutes. Electrospun nanofibrous scaffolds seem to be an appropriate approach as they offer the properties of permeable scaffolds with a high specific surface, the latter one depending on the fiber diameter. Electrospun scaffolds with fiber diameter of 35 nm, 113 nm, 167 nm and 549 nm were manufactured and coated by the layer-by-layer (LbL) technology with either hyaluronic acid or heparin for enhanced regeneration of corneal tissue after surgery. Studies on drug loading capacity and release kinetics defined a lower limit for nanofibrous scaffolds for effective drug loading. Additionally, scaffold characteristics and resulting mechanical properties from the application-oriented characterization of suture pullout from suture retention tests were examined. Finally, scaffolds consisting of nanofibers with a mean fiber diameter of 113 nm were identified as the best-performing scaffolds, concerning drug loading efficiency and resistance against suture pullout.

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Section: Methodsmentioning

confidence: 99%

“…Using a pre-tension of 0.003 N ensures for reliable data. SRT was performed with a constant deformation rate of 10 mm/min, as suggested by Kueng et al [ 24 , 28 ], until complete suture pullout. For comparison of different scaffolds, F max is normalized to the areal weight of each sample, thus a normalized force can be defined as

…”

Section: Methodsmentioning

confidence: 99%

Electrospun PCL Scaffolds as Drug Carrier for Corneal Wound Dressing Using Layer-by-Layer Coating of Hyaluronic Acid and Heparin

Himmler

Schubert²,

Dähne

et al. 2022

IJMS

Self Cite

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“…Suturability of the samples was evaluated by suture retention test, as previously reported [43,44]. One side of the sample, 2 mm away from the peripheral region, was sutured with 10-0 Ethilon nylon suture (Ethicon, USA), and the suture was carefully knotted to make a loop.…”

Section: Evaluation Of Suture Retention Strengthmentioning

confidence: 99%

Decellularized corneal lenticule embedded compressed collagen: toward a suturable collagenous construct for limbal reconstruction

et al. 2018

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Recently, compressed collagen has attracted much attention as a potential alternative for a limbal epithelial stem cell (LESC) carrier to treat limbal stem cell deficiency (LSCD), in that it can provide mechanically improved collagen fibrillar structures compared to conventional collagen hydrogel. However, its clinical efficacy as an LESC carrier has not yet been studied through in vivo transplantation due to limited mechanical strength that cannot withstand a force induced by surgical suturing and low resistance to enzymatic degradation. This study firstly presents a suturable LESC carrier based on compressed collagen in the form of a biocomposite. The biocomposite was achieved by integrating a decellularized corneal lenticule, which is a decellularized stromal tissue obtained from corneal refractive surgery, inside a compressed collagen to form a sandwich structure. A suture retention test verified that the biocomposite has a much higher suture retention strength (0.56 ± 0.12 N) compared to the compressed collagen (0.02 ± 0.01 N). The biocomposite also exhibited more than 3 times higher resistance to enzymatic degradation, indicating long-term stability after transplantation. In vitro cell culture results revealed that the biocomposite effectively supported the expansion and stratification of the LESCs with expressions of putative stem cell and differentiated corneal epithelial cell markers. Finally, the biocomposite verified its clinical efficacy by stably delivering the LESCs onto an eye of a rabbit model of LSCD and effectively reconstructing the ocular surface.

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“…Given these limitations and the occurrence of wound dehiscence, the study adopts the suture retention strength (SRS) test procedure to examine the mechanical resilience of the GHJ. Küng et al [ 19 , 20 ] introduced SRS tests for ophthalmic tissues, where SRS is defined as the load corresponding to the complete tissue rupture [ 21 ]. The similarities between tissue rupture along GHJ and specimen rupture during the SRS test favor the rendition of the SRS tests’ parameters as the strength of the GHJ.…”

Section: Introductionmentioning

confidence: 99%

Investigation of mechanical strength and structure of corneal graft-host junction

Chittajallu,

Gururani,

Jakati

et al. 2024

Heliyon

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Influence of operating parameters on the suture retention test for scaffolds in ophthalmology

Cited by 8 publications

References 15 publications

Electrospun PCL Scaffolds as Drug Carrier for Corneal Wound Dressing Using Layer-by-Layer Coating of Hyaluronic Acid and Heparin

Electrospun PCL Scaffolds as Drug Carrier for Corneal Wound Dressing Using Layer-by-Layer Coating of Hyaluronic Acid and Heparin

Decellularized corneal lenticule embedded compressed collagen: toward a suturable collagenous construct for limbal reconstruction

Investigation of mechanical strength and structure of corneal graft-host junction

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