Influence of orally fed a select mixture of Bacillus probiotics on intestinal T-cell migration in weaned MUC4 resistant pigs following Escherichia coli challenge

Yang, Guo Xiang; Zhu, Yaohong; Zhang, Wei; Zhou, Deliang; Zhai, Cong‐Cong; Wang, Jiufeng

doi:10.1186/s13567-016-0355-8

Cited by 54 publications

(49 citation statements)

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“…We recently found that orally fed probiotic BLS-mix induced the transcription of IL-22 in the small intestine of pigs infected with E. coli (Yang et al, 2016). In the present study, IL-22 production induced by LGG pretreatment further triggered the activation of STAT3, which induced the expression of antimicrobial proteins to defend the mucosal surfaces from invading pathogens (Backert et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

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Oral Administration of Lactobacillus rhamnosus GG Ameliorates Salmonella Infantis-Induced Inflammation in a Pig Model via Activation of the IL-22BP/IL-22/STAT3 Pathway

Yang

et al. 2017

Front. Cell. Infect. Microbiol.

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The high rate of Salmonella enterica serovar Infantis (S. Infantis) infection poses significant risk for the development of non-typhoidal Salmonella gastroenteritis. However, efficient strategies to prevent or treat the infection remain elusive. Here, we explored the effect of the probiotic Lactobacillus rhamnosus GG (LGG) administration in preventing S. Infantis infection in a pig model. Probiotic LGG (1.0 × 1010 CFU/day) was orally administered to newly weaned piglets for 1 week before S. Infantis challenge. LGG pretreatment reduced the severity of diarrhea and alleviated intestinal inflammation caused by S. Infantis. Pre-administration of LGG excluded Salmonella from colonization of the jejunal mucosa but increased the abundance of Bifidobacterium in the feces. LGG promoted the expansion of CD4+ T-bet+ IFNγ+ T cells but attenuated S. Infantis-induced increases in the percentage of CD4+ IFNγ+ T cells and serum interleukin (IL)-22 levels in peripheral blood after S. Infantis challenge. In the small intestine, LGG pretreatment upregulated expression of the transcription factor T-bet but downregulated the S. Infantis-induced increase of CD4+ IFNγ+ T cells in Peyer's patches and IL-7Rα expression in the jejunum. Notably, LGG-treated pigs had enhanced expression of IL-22 and activated STAT3 in the ileum in response to S. Infantis infection. Pretreatment of pigs with LGG also elevated intestinal IL-22-binding protein production in response to S. Infantis challenge. In contrast, LGG consumption reduced the S. Infantis-induced increase in the number of CCL20-expressing cells in the jejunum. Our results suggest that the mechanism by which LGG ameliorates the intestinal inflammation caused by S. Infantis involves the upregulation of T-bet, activation of STAT3, and downregulation of CCL20.

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Section: Discussionmentioning

confidence: 99%

“…Reverse transcription and real-time PCR were performed as previously described (Yang et al, 2016). Data were normalized to the geometric mean C T values of three reference genes: β-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and hypoxanthine phosphoribosyl-transferase.…”

Section: Methodsmentioning

confidence: 99%

Oral Administration of Lactobacillus rhamnosus GG Ameliorates Salmonella Infantis-Induced Inflammation in a Pig Model via Activation of the IL-22BP/IL-22/STAT3 Pathway

Yang

et al. 2017

Front. Cell. Infect. Microbiol.

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“…This study was a further analysis of samples collected during experiments performed in previous works (10,18). The incidence of diarrhea in newly weaned MUC4-resistant pigs was unaffected following F4 ϩ ETEC challenge or probiotic BLS mix treatment.…”

Section: Discussionmentioning

confidence: 99%

“…The experimental model and treatment regimens have been described in detail elsewhere (10,18). In brief, on the day of weaning (day 0), the pigs were divided into five groups of six animals per group and each group received a different treatment, as follows: (i) CONT group, oral administration of sterile physiological saline; (ii) ETEC group, oral administration of sterile physiological saline and oral challenge with F4 ϩ ETEC (1 ϫ 10 10 CFU/day); (iii) LDBE group, oral administration of low-dose probiotic mixture (3.9 ϫ 10 8 CFU/day) and oral challenge with F4 ϩ ETEC; (iv) MDBE group, oral administration of moderate-dose probiotic mixture (7.8 ϫ 10 8 CFU/day) and oral challenge with F4 ϩ ETEC; and (v) HDBE group, oral administration of high-dose probiotic mixture (3.9 ϫ 10 9 CFU/day) and oral challenge with F4 ϩ ETEC.…”

Section: Methodsmentioning

confidence: 99%

Oral Administration of a Select Mixture of Bacillus Probiotics Affects the Gut Microbiota and Goblet Cell Function following Escherichia coli Challenge in Newly Weaned Pigs of Genotype MUC4 That Are Supposed To Be Enterotoxigenic E. coli F4ab/ac Receptor Negative

Zhang

Zhu

Zhou

et al. 2017

Appl Environ Microbiol

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Structural disruption of the gut microbiota and impaired goblet cell function are collateral etiologic factors in enteric diseases. Low, moderate, or high doses of a Bacillus licheniformis-B. subtilis mixture (BLS mix) were orally administered to piglets of genotype MUC4 that are supposed to be F4-expressing enterotoxigenic Escherichia coli strain (F4 ϩ ETEC) F4ab/ac receptor negative (i.e., MUC4-resistant piglets) for 1 week before F4 ϩ ETEC challenge. The luminal contents were collected from the mucosa of the colon on day 8 after F4 ϩ ETEC challenge. The BLS mix attenuated E. coli-induced expansion of Bacteroides uniformis, Eubacterium eligens, Acetanaerobacterium, and Sporobacter populations. Clostridium and Turicibacter populations increased following F4 ϩ ETEC challenge in pigs pretreated with low-dose BLS mix. Lactobacillus gasseri and Lactobacillus salivarius populations increased after administration of BLS mix during E. coli infection. The beneficial effects of BLS mix were due in part to the expansion of certain Clostridium, Lactobacillus, and Turicibacter populations, with a corresponding increase in the number of goblet cells in the ileum via upregulated Atoh1 expression, in turn increasing MUC2 production and thus preserving the mucus barrier and enhancing host defenses against enteropathogenic bacteria. However, excessive BLS mix consumption may increase the risk for enteritis, partly through disruption of colonic microbial ecology, characterized by expansion of Proteobacteria and impaired goblet cell function in the ileum. Our findings suggest that oral administration of BLS mix reprograms the gut microbiota and enhances goblet cell function to ameliorate enteritis.IMPORTANCE The present study is important for improving our understanding of the protective role of probiotics against Escherichia coli infection in piglets. Structural disruption of the gut microbiota and impaired goblet cell function are collateral etiologic factors in enteric diseases. In this study, low, moderate, or high doses of a Bacillus licheniformis-B. subtilis mixture (BLS mix) were orally administered to MUC4-resistant piglets for 1 week before the F4-expressing ETEC strain (F4 ϩ ETEC) challenge. Our findings suggest that oral administration of BLS mix reprograms the gut microbiota and enhances goblet cell function to ameliorate enteritis. KEYWORDS

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“…However, subsequent and more recent studies have been conducted with probiotics to study the effect on intestinal immune responses under challenge of the pigs (e.g. Yang et al 2016), and more scientific knowledge is available on the fundamental mechanisms of the potential immunomodulatory effects of the feed additives.…”

Section: Introductionmentioning

confidence: 99%

Immunomodulating effects of probiotics for microbiota modulation, gut health and disease resistance in pigs

Roselli

Pieper

Rogel–Gaillard

et al. 2017

Animal Feed Science and Technology

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General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms HIGHLIGHTS-Updated literature overview about probiotics, prebiotics and synbiotics on pig gut health is provided -Both in vivo studies performed in pigs and in vitro studies conducted in pig intestinal cell lines are described -A critical outcome of the described results is provided -The concept of "proxy measurements" for pig gut health is widely discussed -The link between gut microbiota and mucsal immune system is described Abstract:Probiotics are live microorganisms that can confer a health benefit on the host, and amongst various mechanisms probiotics are believed to exert their effects by production of antimicrobial substances, competition with pathogens for adhesion sites and nutrients, enhancement of mucosal barrier integrity and immune modulation. Through these activities probiotics can support three core benefits for the host: supporting a healthy gut microbiota, a healthy digestive tract and a healthy immune system. More recently, the concept of combining probiotics and prebiotics, i.e. synbiotics, for the beneficial effect on gut health of pigs has attracted major interest, and examples of probiotic and prebiotic benefits for pigs are pathogen inhibition and immunomodulation. Yet, it remains to be defined in pigs, what exactly is a healthy gut. Because of the high level of variability in growth and feed conversion between individual pigs in commercial production systems, measuring the impact of probiotics on gut health defined by improvements in overall productivity requires large experiments. For this reason, many studies have concentrated on measuring the effects of the feed additives on proxies of gut health including many immunological measures, in more controlled experiments. With the major focus of studying the balance between gut microbiology, immunology and physiology, and the potential for prevention of intestinal disorders in pigs, we therefore performed a literature review of the immunomodulatory effects of probiotics, either alone or in combination with prebiotics, based on in vivo, in vitro and ex vivo porcine experiments. A 3 consistent number of studies showed the potential capacity in terms of immunomodulatory activities of these feed additives in pigs, but contrasting results can also be obtained from the literature.Reasons for this are not clear but could be related to differences with respect to the probiotic strain used, experimental settings, diets, initial microbiota colonization, administration route, time and frequency of administration of the probiotic strain and sampling for analysis. Hence, the use of proxy measurements of enteric health based on observable immunological parameters presents significant problems at the moment, and cannot be considered robust, reliable predictors of the probiotic activity in vivo, in relation to pig gut health. In c...

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Influence of orally fed a select mixture of Bacillus probiotics on intestinal T-cell migration in weaned MUC4 resistant pigs following Escherichia coli challenge

Cited by 54 publications

References 57 publications

Oral Administration of Lactobacillus rhamnosus GG Ameliorates Salmonella Infantis-Induced Inflammation in a Pig Model via Activation of the IL-22BP/IL-22/STAT3 Pathway

Oral Administration of Lactobacillus rhamnosus GG Ameliorates Salmonella Infantis-Induced Inflammation in a Pig Model via Activation of the IL-22BP/IL-22/STAT3 Pathway

Oral Administration of a Select Mixture of Bacillus Probiotics Affects the Gut Microbiota and Goblet Cell Function following Escherichia coli Challenge in Newly Weaned Pigs of Genotype MUC4 That Are Supposed To Be Enterotoxigenic E. coli F4ab/ac Receptor Negative

Immunomodulating effects of probiotics for microbiota modulation, gut health and disease resistance in pigs

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