Influence of osmotic pressure and viscosity on intestinal drug absorption. I. Studies on the gastric effluent following oral administration of various quinine solutions to rats.

Sakiya, Yoko; Miyauchi, Yoshiko; Tsuemura, Yoshiji

doi:10.1248/cpb.29.539

Cited by 3 publications

(1 citation statement)

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“…It was reported that hypotonic solution played a significant role in the absorption of polar and slowly absorbable drugs (Kojima & Miyake, ). It was discovered that the amount of quinine absorbed was highest from hypotonic to isotonic solutions and decreased with increasing osmotic pressure (Sakiya, Miyauchi, & Tsuemura, , ). Statistically significant correlations among the hypertonic solutions between the reciprocal of osmotic pressure and the area under the Concentration‐Time or curve C max : the peak concentration (AUC 0 – ∞ or C max ) of sulfafurazole in rat were found (Marvola, Reinikainen, Heliövaara, & Huikari, ).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of eight B‐vitamins in rat intestinal perfusate to identify effects of osmotic pressures on absorptions

Wang

Jian-yun²,

Zhou

et al. 2017

Biomedical Chromatography

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A rapid and accurate HPLC-DAD method was developed and validated to simultaneously determine eight B-vitamins (VBs, namely thiamine, riboflavin, niacinamide, calcium pantothenic, pyridoxine, biotin, folic acid and cyanocobalamin) and phenolsulfonphthalein in rat intestinal perfusate. Chromatographic separation was achieved using an Inertsil ODS-3 column (250 × 4.6 mm i.d., 5 μm) at a temperature of 40°C. Gradient elution mode was applied at the flow rate of 1.0 mL/min with the mobile phase of acetonitrile-30 mm K HPO (pH 5.80). The method was successfully applied to identify the effects of osmotic pressures on the absorption of the VBs. The absorption profiles of single and mixed VBs were also compared. Histological section technology was applied to observe the microstructure of small bowel mucosa after perfusion. The results indicated that each compound possessed a better absorption profile under isotonic conditions than under hypotonic or hypertonic conditions for single or mixed solutions. Compared with single VBs, better absorptions in mixed VBs were observed. Pathological tissue slice test suggested that hypotonic and hypertonic solutions changed or damaged the microstructure of mucosa to varying degrees. Taken together, the investigations indicated that multi-VBs administered orally under isotonic condition could generate fast and complete absorption profiles for VBs.

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Section: Introductionmentioning

confidence: 99%