Influence of oxidative homeostasis on bacterial density and cost of infection in <i>Drosophila</i>–<i>Wolbachia</i> symbioses

Monnin, David; Kremer, Natacha; Berny, Clément; Henri, Hélène; Dumet, Adeline; Voituron, Yann; Desouhant, Emmanuel; Vavre, Fabrice

doi:10.1111/jeb.12863

Cited by 17 publications

(21 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The evolution of symbioses can be influenced by the oxidative homeostasis (i.e., the balance between reactive oxygen species (ROS) and antioxidant molecules) (Moné, Monnin & Kremer, 2014; Monnin et al, 2016). Indeed, ROS are toxic effectors that can participate in the host immune defense to regulate symbiont populations.…”

Section: Resultsmentioning

confidence: 99%

Toward a better understanding of the mechanisms of symbiosis: a comprehensive proteome map of a nascent insect symbiont

Renoz

Champagne

Degand

et al. 2017

PeerJ

View full text Add to dashboard Cite

Symbiotic bacteria are common in insects and can affect various aspects of their hosts' biology. Although the effects of insect symbionts have been clarified for various insect symbiosis models, due to the difficulty of cultivating them in vitro, there is still limited knowledge available on the molecular features that drive symbiosis. Serratia symbiotica is one of the most common symbionts found in aphids. The recent findings of freeliving strains that are considered as nascent partners of aphids provide the opportunity to examine the molecular mechanisms that a symbiont can deploy at the early stages of the symbiosis (i.e., symbiotic factors). In this work, a proteomic approach was used to establish a comprehensive proteome map of the free-living S. symbiotica strain CWBI-2.3 T . Most of the 720 proteins identified are related to housekeeping or primary metabolism. Of these, 76 were identified as candidate proteins possibly promoting host colonization. Our results provide strong evidence that S. symbiotica CWBI-2.3 T is well-armed for invading insect host tissues, and suggest that certain molecular features usually harbored by pathogenic bacteria are no longer present. This comprehensive proteome map provides a series of candidate genes for further studies to understand the molecular cross-talk between insects and symbiotic bacteria.

show abstract

Section: Resultsmentioning

confidence: 99%

Toward a better understanding of the mechanisms of symbiosis: a comprehensive proteome map of a nascent insect symbiont

Renoz

Champagne

Degand

et al. 2017

PeerJ

View full text Add to dashboard Cite

show abstract

“…In the present study, we used experimental evolution over 17 generations to test these hypotheses, raising w MelPop infected flies at 29°C, and enforcing reproduction eight days after emergence. In addition, in one experimental condition, we fed the flies with paraquat, as it was previously shown to increase the survival of w MelPop-infected flies (while having no effect on the survival of uninfected flies) and to reduce the symbiotic density (Monnin et al 2016). We hypothesised that this treatment would reduce or cancel the selective pressure for reduced virulence, density, and octomom copy number, resulting in higher values for these parameters, compared to the control condition.…”

Section: Introductionmentioning

confidence: 99%

Experimental evolution of virulence and associated traits in aDrosophila melanogaster–Wolbachiasymbiosis

Monnin

Kremer

Michaud

et al. 2020

Preprint

View full text Add to dashboard Cite

13Evolutionary theory predicts that vertically transmitted symbionts are selected for low 14 virulence, as their fitness is directly correlated to that of their host. In contrast with this prediction, 15 the Wolbachia strain wMelPop drastically reduces its Drosophila melanogaster host lifespan at 16 high rearing temperatures. It is generally assumed that this feature is maintained because the 17 D. melanogaster-wMelPop symbiosis is usually not exposed to environmental conditions in which 18 the symbiont is virulent. To test this hypothesis, we submitted wMelPop-infected D. melanogaster 19 lines to 17 generations of experimental evolution at a high temperature, while enforcing late 20 reproduction. The fly survival was measured at different time points, as well as two traits that have 21 2 been proposed to be causally responsible for wMelPop virulence: its relative density and the mean 22 number of octomom copies present in its genome. We hypothesised that these conditions would 23 select for a reduced wMelPop virulence, a reduced wMelPop density, and a reduced octomom copy 24 number. Our results indicate that density, octomom copy number and virulence are correlated. 25However, contrary to our expectations, we could not detect any reduction in virulence during the 26 course of evolution. We discuss the significance of our results with respect to the evolutionary 27 causes of wMelPop virulence and propose that intra-host selection could explain this conundrum. 28 29 42correlated to that of their hosts, they are expected to be selected for low virulence (Ewald 1983). 43 3 This reasoning, just like the transmission/virulence trade-off hypothesis, rests solely on the 44inter-host level of selection, ignoring the potential effects of intra-host selection. Indeed, the most 45 competitive symbionts within a host may replicate faster and be more virulent than what would be 46 optimal from the standpoint of inter-host selection (Alizon et al. 2013). However, the causal link 47

show abstract

“…Wolbachia is seen to affect lifespan (Maklakov et al, 2006), sometimes increasing lifespan (Dobson et al, 2004) and sometimes decreasing it (Fleury et al, 2000). Wolbachia has interactions with the immune system via ROS in Drosophila (Zug & Hammerstein, 2015) by inducing the expression of host antioxidant proteins (Brennan et al, 2008), by being influenced by oxidative homeostasis (Monnin et al, 2016) and by antiviral protection (Wong et al, 2015). In mosquitos, Wolbachia also affects immunity via ROS (Pan et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Wolbachia affects survival to different oxidative stressors dependent upon the genetic background in Drosophila melanogaster

2018

View full text Add to dashboard Cite

It is well known that the rate of ageing varies among individuals dependent on the genetic background. In the present study, we explore how Wolbachia infection (a common insect endosymbiont bacterium) and oxidative stress interact in ageing with respect to two different genetic backgrounds of Drosophila melanogaster. Naturally infected and cured lines of Drosophila are challenged with either paraquat or l‐arginine to generate two different types of oxidative stress. We first observe that removing Wolbachia infection shortens the lifespan in one genetic background but not in the other. Wolbachia infection only makes one of the genetic lines more sensitive to paraquat. However, only the line unaffected by Wolbachia in the paraquat treatment is protected by Wolbachia from l‐arginine induced stress. Hence, Wolbachia is modifying free radical defence via two different mechanisms dependent on the genetic background. This supports the idea that factors that can govern ageing (infection and oxidative stress) are not universal, but are specific to the genetic make‐up of an individual.

show abstract

Influence of oxidative homeostasis on bacterial density and cost of infection in Drosophila–Wolbachia symbioses

Cited by 17 publications

References 57 publications

Toward a better understanding of the mechanisms of symbiosis: a comprehensive proteome map of a nascent insect symbiont

Toward a better understanding of the mechanisms of symbiosis: a comprehensive proteome map of a nascent insect symbiont

Experimental evolution of virulence and associated traits in aDrosophila melanogaster–Wolbachiasymbiosis

Wolbachia affects survival to different oxidative stressors dependent upon the genetic background in Drosophila melanogaster

Contact Info

Product

Resources

About