Influence of Oxygen and Acetylene during in situ Open-flow Assays of Nitrogenase Activity (C2H2 Reduction) in Phaseolus vulgaris Root Nodules

Hernández, Germán Fernando Gutiérrez; Drevon, Jean‐Jacques

doi:10.1016/s0176-1617(11)80246-4

Cited by 26 publications

(12 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, in the previous work, nodules were maintained in the gaseous phase, emerged from the nutrient solution whereas in this work, nodules were immersed in the nutrient solution, which could result in an alteration of gas diffusion towards and inside nodules. The latter is also suggested by the comparison of ARA response curves to pO 2 , showing higher COP for genotype B in this work than in the previous work with emerged nodules [15]. However, there was no difference between both methodologies for ARA lower than 300 µmol⋅h -1 ⋅plant -1 in soybean (Drevon and Vidal, pers.…”

Section: Discussionsupporting

confidence: 78%

“…Satisfactory common bean growth and N 2 fixation have been obtained in hydroponic culture [15] where genotypes differing in seed coat color showed variability in their nodulation and nodule activity in symbiosis with rhizobium [16]. Moreover, polyphenolic compounds related to seed-coat color have been involved in the establishment of the rhizobial symbiosis with common bean [17,21].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genotypic difference in the short-term response of nitrogenase activity (C₂H₂ reduction) to salinity and oxygen in the common bean

et al. 2001

Self Cite

View full text Add to dashboard Cite

difference in the short-term response of nitrogenase activity (C_2H_2 reduction) to salinity and oxygen in the common bean. Agronomie, EDP Sciences, 2001, 21 (6- Abstract -Four genotypes of common bean (Phaseolus vulgaris L.) differing in seed color, were grown hydroponically in a growth chamber to study the short-term effects of 0.1 M NaCl on their symbiotic acetylene reducing activity (ARA). For all genotypes, the ARA was reduced dramatically during the first hour following NaCl application. This was followed by a slower decrease during the next 5 h during which genotypical differences in response to salinity were found. When expressed as percent of the control, the redseed genotype was significantly less inhibited by NaCl than the three others. For all genotypes, the increase in ARA after raising rhizosphere pO 2 was affected by the NaCl treatment. With NaCl the red-seed genotype showed the highest stimulation by pO 2 , which was associated with its higher tolerance to NaCl application. However for all genotypes, raising pO 2 did not allow a complete recovery from the NaCl effect. It is concluded that nitrogenase inhibition by NaCl application in common bean varies among genotypes, and is associated with a short-term nodule limitation by O 2 diffusion, although other mechanisms may also be involved in nodule inhibition by NaCl. N 2 fixation / oxygen diffusion / Phaseolus vulgaris / Rhizobium / salinity Résumé -Effets à court terme de la salinité sur l'activité nitrogénase nodulaire et sa réponse à l'oxygène chez le haricot. Quatre génotypes de haricot (Phaseolus vulgaris L.) différant par la couleur des téguments de leur graine, ont été cultivés hydroponiquement en chambre de culture afin d'étudier les effets de NaCl sur leur fixation symbiotique d'azote et leur croissance. L'activité réductrice de l'acétylène (ARA) était fortement réduite pendant la première heure suivant l'application de NaCl. Cette baisse initiale était suivie d'une baisse plus lente durant laquelle des différences variétales ont été mesurées. Le génotype R était significativement moins inhibé par NaCl que les 3 autres. Pour tous les génotypes, la stimulation d'ARA par augmentation de la pO 2 rhizosphérique était modifiée par le traitement salin. Avec NaCl, le génotype R présenta la plus forte stimulation par la pO 2 , qui était associée à sa plus forte tolérance à NaCl. Toutefois quel que soit le génotype, l'élévation de pO 2 ne compensa pas totalement l'inhibition par NaCl. Il est conclu que l'inhibition de la nitrogénase par la salinité chez le haricot fixateur d'azote varie entre génotypes, et est associée à une limitation du métabolisme nodulaire par un manque d'O 2 , bien que d'autres mécanismes d'inhibition par NaCl puissent être impliqués.

show abstract

Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Genotypic difference in the short-term response of nitrogenase activity (C₂H₂ reduction) to salinity and oxygen in the common bean

et al. 2001

Self Cite

View full text Add to dashboard Cite

show abstract

“…Each seedling was carefully transplanted to a 0.40×0.20×0.20 m container that keeps the rooting environment in darkness. The roots were gently passed through the hole of a rubber stopper on the container neck, and cotton wool was fitted at the hypocotyl level (Hernandez and Drevon 1991). Each container contained nutrient solution of the following composition (μM): K 2 SO 4 , 700; MgSO 4 , 500; CaCl 2 , 800; H 3 BO 3 , 4; Na 2 MoO 4 , 0.1; ZnSO 4 , 1; MnCl 2 , 2; CoCl 2 , 0.2; CuCl 2 , 1; FeNaEDTA (ferric monosodium salt of ethylenediamine tetra acetic acid), 10; and MES [2-(N-morpholino) ethane-sulfonic acid], 200.…”

Section: Hydroaeroponic Culture Experimentsmentioning

confidence: 99%

Variation for nodulation and plant yield of common bean genotypes and environmental effects on the genotype expression

Rodiño¹,

Fuente²,

Ron³

et al. 2011

Plant Soil

View full text Add to dashboard Cite

Common bean symbiotic nitrogen fixation provides an ecological and economical alternative to increase bean production but it depends on soil fertility and climate conditions. The objectives of this work were to characterize common bean genotypes for their ability to establish symbiosis under controlled conditions and to study the effect of the environment on the expression of those genotypes. The experiment under controlled conditions was conducted in a greenhouse with 158 genotypes that represented the major dry bean market classes. The field experiment was carried out in six environments with 64 genotypes that were previously selected for their contrasting nodulation ability and/or root development under the controlled conditions experiment. Nodulation, plant, and grain yield data of the bean genotypes were measured in both experiments. There was a significant high variability in plant development responses among the studied genotypes associated with the rhizobial strain inoculated under controlled conditions. Two nodulation phenotypes were observed among the genotypes tested: the big-nodules phenotype (BNO) associated with almost 63% fewer nodules and 58% higher proportion of nodule biomass in the below-ground compartment than the smallnodules phenotype (SNO) with less developed aerial parts. Genotype plus genotype × environment (GGE) biplot model analysis enabled identification of the highest-yielding genotypes for the different environments. The soil chemical factors of these environments were associated with the nodule number or the biomass of the common bean genotypes. Genotypes with a BNO phenotype showed a good plant response, indicating that this phenotype may be more beneficial for plant growth and seed yield in environmental conditions that may limit nodule development. The amplitude of the genotypic variability found in this work confirms the potential for rhizobial symbiosis of adapted bean genotypes, which could constitute a preferential material for initial breeding of symbiotically active lines. The data also indicate the potential of bean breeding to identify environments containing effective strains of rhizobia essential for sustainable agriculture, improving productivity, and maintaining environmental quality.

show abstract

“…After germination homogenous seedlings were transferred into a temperature-controlled glasshouse as described previously by Hernandez and Drevon (1991). The root of inoculated seedling was passed carefully through pierced rubber stopper, fixed with cotton fitted around the hypocotyls, and placed upon the topper of a 0040 x 0.20 x 0.20 m vat receiving 15 plants with 15 1 of the Vadez et al (1996) nutrition solution, and complemented to 40 1 with sterile distilled water.…”

Section: Hydroaeroponic Culturementioning

confidence: 99%

Variation among common-bean accessions (Phaseolus vulgaris L.) from the Iberian Peninsula for N2-dependent growth and phosphorus requirement

et al. 2009

View full text Add to dashboard Cite

The common-bean often faces phosphorus deficiency in soils where it is grown. Such a deficiency is a major limitation to yield improvement, especially as the common-bean depends upon nitrogen fixation. Screening for symbiotic nitrogen fixation under phosphorus deficiency was performed with 33 common-bean accessions representing the diversity of 15 European market classes from the Iberian Peninsula. These accessions were inoculated with Rhizobium tropici CIAT899 and grown in an aerated nitrogen-free nutrient solution at deficient versus sufficient phosphorous supplies (75 vs. 250 urnol plant" week-I) in a glasshouse. A large variability in N 2-dependent growth under P deficiency was found with most tolerance to P deficiency among late type IV Andean landraces, with the exception of 3 early type I Andean landraces. From this screening four contrasting landraces were selected for their high efficiency in the use of P for their symbiotic N nutrition, and compared with the cultivar Linex in fields of a reference production area over 3 years. The landraces from the Iberian Peninsula expressed a higher growth than the cultivar Linex, although they showed a lower nodulation. We hypothesize that the identified P tolerance among Iberian Peninsula accessions may be useful for improving symbiotic nitrogen fixation in the common-bean when growth is limited by available soil-P and could contribute to sustainable farming systems by reducing farmers' dependence on fertilizers.

show abstract

Influence of Oxygen and Acetylene during in situ Open-flow Assays of Nitrogenase Activity (C2H2 Reduction) in Phaseolus vulgaris Root Nodules

Cited by 26 publications

References 13 publications

Genotypic difference in the short-term response of nitrogenase activity (C₂H₂ reduction) to salinity and oxygen in the common bean

Genotypic difference in the short-term response of nitrogenase activity (C₂H₂ reduction) to salinity and oxygen in the common bean

Variation for nodulation and plant yield of common bean genotypes and environmental effects on the genotype expression

Variation among common-bean accessions (Phaseolus vulgaris L.) from the Iberian Peninsula for N2-dependent growth and phosphorus requirement

Contact Info

Product

Resources

About

Influence of Oxygen and Acetylene during in situ Open-flow Assays of Nitrogenase Activity (C2H2 Reduction) in Phaseolus vulgaris Root Nodules

Cited by 26 publications

References 13 publications

Genotypic difference in the short-term response of nitrogenase activity (C2H2 reduction) to salinity and oxygen in the common bean

Genotypic difference in the short-term response of nitrogenase activity (C2H2 reduction) to salinity and oxygen in the common bean

Variation for nodulation and plant yield of common bean genotypes and environmental effects on the genotype expression

Variation among common-bean accessions (Phaseolus vulgaris L.) from the Iberian Peninsula for N2-dependent growth and phosphorus requirement

Contact Info

Product

Resources

About

Genotypic difference in the short-term response of nitrogenase activity (C₂H₂ reduction) to salinity and oxygen in the common bean

Genotypic difference in the short-term response of nitrogenase activity (C₂H₂ reduction) to salinity and oxygen in the common bean