Influence of oyster genetic background on levels of human-pathogenic Vibrio spp.

Audemard, Corinne; Reece, Kimberly S.; Latour, Robert J.; Bienlien, Lydia M.; Carnegie, Ryan B.

doi:10.1016/j.aquaculture.2022.738763

Cited by 3 publications

(3 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These data suggest that the trends observed in this study are stable over-time or at least under this time scale. Differential in situ dynamics between total and pathogenic V. parahaemolyticus strains has been observed in other studies [ 9 , 51 , 55 , 56 , 57 ]. While the actual biotic and/or abiotic factors driving these different in situ responses remain to be identified, results of in vivo studies exploring the role of genes associated with pathogenic strains suggest that the role of biotic interactions should be further investigated [ 58 , 59 ].…”

Section: Discussionsupporting

confidence: 75%

“…Within our studied sites, pathogenic V. parahaemolyticus concentrations peaked in the water and in oysters in June and then declined, while concentrations of total V. parahaemolyticus remained elevated during July, August and September in most samples and started decreasing in the fall. Other studies conducted within the lower Chesapeake Bay region and relying on samples collected from 2014 to 2019 have highlighted similar dynamics of these bacteria in oysters [ 43 , 54 , 55 ]. These data suggest that the trends observed in this study are stable over-time or at least under this time scale.…”

Section: Discussionmentioning

confidence: 86%

“…Previous studies focusing on individual oysters have shown that levels of human-pathogenic Vibrio spp. can vary over several orders of magnitude, suggesting that the influence of the oyster itself cannot be discounted [ 54 , 55 , 56 , 61 , 62 , 63 ]. Additionally, higher concentrations of these bacteria measured in oysters compared to water samples facilitate detection in oyster samples, especially with regard to tdh + and trh + strains.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Vibrio vulnificus and Vibrio parahaemolyticus in Oysters under Low Tidal Range Conditions: Is Seawater Analysis Useful for Risk Assessment?

Audemard

Ben‐Horin

Kator

et al. 2022

Foods

Self Cite

View full text Add to dashboard Cite

Human-pathogenic Vibrio bacteria are acquired by oysters through filtering seawater, however, the relationships between levels of these bacteria in measured in oysters and overlying waters are inconsistent across regions. The reasons for these discrepancies are unclear hindering our ability to assess if -or when- seawater samples can be used as a proxy for oysters to assess risk. We investigated whether concentrations of total and human pathogenic Vibrio vulnificus (vvhA and pilF genes) and Vibrio parahaemolyticus (tlh, tdh and trh genes) measured in seawater reflect concentrations of these bacteria in oysters (Crassostrea virginica) cultured within the US lower Chesapeake Bay region. We measured Vibrio spp. concentrations using an MPN-qPCR approach and analyzed the data using structural equation modeling (SEM). We found seawater concentrations of these bacteria to predictably respond to temperature and salinity over chlorophyll a, pheophytin or turbidity. We also inferred from the SEM results that Vibrio concentrations in seawater strongly predict their respective concentrations in oysters. We hypothesize that such seawater-oyster coupling can be observed in regions of low tidal range. Due to the ease of sampling and processing of seawater samples compared to oyster samples, we suggest that under low tidal range conditions, seawater samples can foster increased spatial and temporal coverage and complement data associated with oyster samples.

show abstract

Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Vibrio vulnificus and Vibrio parahaemolyticus in Oysters under Low Tidal Range Conditions: Is Seawater Analysis Useful for Risk Assessment?

Audemard

Ben‐Horin

Kator

et al. 2022

Foods

Self Cite

View full text Add to dashboard Cite

show abstract

The need for more information on the resistance to biological and environmental stressors in triploid oysters

Brianik¹,

Allam²

2023

Aquaculture

View full text Add to dashboard Cite

Bivalve microbiomes are shaped by host species, size, parasite infection, and environment

Gignoux-Wolfsohn,

Garcia Ruiz,

Portugal Barron

et al. 2024

PeerJ

View full text Add to dashboard Cite

Many factors affect an organism’s microbiome including its environment, proximity to other organisms, and physiological condition. As filter feeders, bivalves have highly plastic microbiomes that are especially influenced by the surrounding seawater, yet they also maintain a unique core set of microbes. Using 16S ribosomal RNA sequencing, we characterized the bacterial microbiomes of four species of bivalves native to the Mid-Atlantic East Coast of North America: Crassostrea virginica, Macoma balthica, Ameritella mitchelli, and Ischadium recurvum and assessed the impact of their external environment, internal parasites, and size on their microbial communities. We found significant differences in bacterial amplicon sequence variants (ASVs) across species, with each species harboring a core ASV present across all individuals. We further found that some C. virginica co-cultured with I. recurvum had high abundances of the I. recurvum core ASV. We identified ASVs associated with infection by the parasites Perkinsus marinus and Zaops ostreum as well others associated with bivalve size. Several of these ASV are candidates for further investigation as potential probiotics, as they were found positively correlated with bivalve size and health. This research represents the first description of the microbiomes of A. mitchelli, I. recurvum, and M. balthica. We document that all four species have highly plastic microbiomes, while maintaining certain core bacteria, with important implications for growth, health, and adaptation to new environments.

show abstract

Influence of oyster genetic background on levels of human-pathogenic Vibrio spp.

Cited by 3 publications

References 50 publications

Vibrio vulnificus and Vibrio parahaemolyticus in Oysters under Low Tidal Range Conditions: Is Seawater Analysis Useful for Risk Assessment?

Vibrio vulnificus and Vibrio parahaemolyticus in Oysters under Low Tidal Range Conditions: Is Seawater Analysis Useful for Risk Assessment?

The need for more information on the resistance to biological and environmental stressors in triploid oysters

Bivalve microbiomes are shaped by host species, size, parasite infection, and environment

Contact Info

Product

Resources

About