Influence of partially purified extracts ofSolanum malacoxylon on bone resorption in organ culture

Liskova-Kiar, Maria; Proschek, L.

doi:10.1007/bf02013232

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…The mechanism of action of E on bone is uncertain despite studies over the past three decades reporting effects on bone in rodents and quail and on mineral metabolism in humans (1)(2)(3)(4)(5)(20)(21)(22)(23)(24)(25)(26)(27)(28). The studies in rodents and quail have found effects of exogenous estrogens on osteoblasts, their precursors, or both (20)(21)(22)(23)(24)(25)(26)(27)(28).…”

Section: Discussionmentioning

confidence: 99%

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17 beta-estradiol acts directly on the clonal osteoblastic cell line UMR106.

Gray¹,

Flynn²,

Gray³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

166

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We studied the effect of 17f8-estradiol (E) on the proliferation and alkaline phosphatase activity of cultured UMR106 cells, a clonal osteoblastic cell line. Growth rates were reduced and alkaline phosphatase activity was increased in cells incubated for 2 days in medium containing E (10-8 M). In contrast, E had no effect on the growth rates or alkaline phosphatase of a human fibroblastic cell line, S90E. The effect of E was not observed with low cell density or at confluence. 1,25-Dihydroxyvitamin D3 antagonized the response to E. Preincubation of the cells with dexamethasone, a potent inducer of differentiation, reversed the effect of E or 1,25-dihydroxyvitamin D3. These results indicate that cellular and/or extracellular factors such as cell density, the phase of the cell cycle, the state of differentiation, and the presence or absence of other steroids influenced the response of UMR106 cells to E. Serum was removed from the culture medium to minimize the effect of the steroids, growth factors, and nutrients present in serum. A striking stimulation of alkaline phosphatase by E occurred with serum-free conditions. This stimulation was biphasic over an E concentration from 101-2 to 10-8 M, with the peak response at 10-1' M. The action of E on UMR106 cells was metabolite-specific, since the isomer 17a-estradiol produced no effect on proliferation rates or alkaline phosphatase activity. The cyclic AMP response to parathyroid hormone (residues 1-34) was not altered by E treatment of these cells. In contrast, dexamethasone exposure did increase the cyclic AMP response to parathyroid hormone. These results demonstrate a direct effect of E on an osteoblastic cell line. They also raise the possibility that similar or identical actions of E occur in cultured normal osteoblasts.Estrogens influence the skeleton, as evidenced by the loss of bone density in postmenopausal women (1, 2) and the preventative action of exogenous estrogens on the loss of bone density in ovariectomized subjects (3). Estrogen administration also produces changes related to bone and mineral metabolism, such as increased intestinal absorption of calcium (4) and alterations in the circulating calciotropic hormones (4, 5). The mechanism of estrogens' action on the skeleton is still unknown. Most investigators have postulated an indirect pathway because of the apparent absence of estrogen receptors in bone (6-9). We report the results of experiments showing an effect of 17p-estradiol (E) on the UMR106 cell line, a well-characterized osteoblastic cell model (10-12). MATERIALS AND METHODSMaterials. The culture media and additives were obtained from the Tissue Culture Facility of the Lineberger Cancer Research Center, University of North Carolina. The additives included fetal bovine serum, penicillin, streptomycin, insulin, transferrin, selenium, and trypsin, the latter for release of adherent cells. E and 17a-estradiol (17a-E) and dexamethasone were purchased from Sigma. Parathyroid hormone, as the synthetic fragment consisting of amino acid res...

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Section: Discussionmentioning

confidence: 99%

“…The studies in rodents and quail have found effects of exogenous estrogens on osteoblasts, their precursors, or both (20)(21)(22)(23)(24)(25)(26)(27)(28). Endosteal proliferation and metaphyseal sclerosis were observed in several studies, implying an anatomic localization of the estrogen effect.…”

Section: Discussionmentioning

confidence: 99%

17 beta-estradiol acts directly on the clonal osteoblastic cell line UMR106.

Gray¹,

Flynn²,

Gray³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

166

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“…In vitro assays using S. glaucophyllum Desf. extract were performed only on bone fragment cultures (Liskova‐Kiar & Proschek, 1978; Puche & Locatto, 1974; Stern, Ness, & Luca, 1977) in order to understand the effects of the plant in terms of bone resorption. However, because the osteoblast is a cell derived from osteogenic differentiation of bone marrow mesenchymal stem cells (MSCs), one of the hypotheses of this study was that osteoporosis induced in vivo by S. glaucophyllum Desf.…”

Section: Introductionmentioning

confidence: 99%

The Solanum glaucophyllum Desf. extract reduces mineralized matrix synthesis in osteogenically differentiated rat mesenchymal stem cells in vitro

Melo

Ocarino

Reis

et al. 2020

Animal Physiology Nutrition

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The Solanum glaucophyllum Desf. has been used to treat and prevent diseases in human and veterinary medicine. On the other hand, plant poisoning causes several bone diseases, among them osteoporosis, which is characterized by osteoblastic hypoplasia. Because the osteoblast is a cell derived from the differentiation of mesenchymal stem cells (MSCs) from bone marrow, the hypothesis is that the plant reduces the osteogenic differentiation of MSCs. The objective of this study was to evaluate the effects of S. glaucophyllum Desf. extract on MSCs cultured in osteogenic differentiation medium. We determined by liquid chromatography that 1 ml of plant extract contained 3.8 μl of 1,25(OH)2D3 (calcitriol). Four groups of MSCs cultivated in osteogenic medium were evaluated as follows: (a) treated with 100 μl of extract/L containing 0.4 μg/L of calcitriol; (b) treated with 1 ml of extract/L containing 4 μg/L of calcitriol; (c) treated with 5 ml of extract/L containing 20 μg/L of calcitriol; and (d) a control group without extract. We performed alkaline phosphatase activity assay, analysis of MTT conversion to formazan, and evaluated the percentage of cells, and number and diameter of mineralization nodules. The expression of gene transcripts for osteopontin, bone sialoprotein and BMP‐2 was analysed by RT‐qPCR. After 21 days, there was a significant reduction in MTT conversion to formazan in treated groups, of the cellularity in the group with 5 ml of extract/L, and in the number and size of mineralization nodules in the groups treated with 1 and 5 ml of extract/L. The 5 ml extract/L concentration also reduced transcript expression of osteopontin. It is concluded that S. glaucophyllum Desf. at concentrations of 1 and 5 ml extract/L reduced mineralized matrix synthesis in MSCs cultivated in osteogenic differentiation medium, which suggests that this is one of the mechanisms by which osteoporosis occurs in intoxicated animals.

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Phosphorus utilization in domestic animals

Cheng

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Influence of partially purified extracts ofSolanum malacoxylon on bone resorption in organ culture

Cited by 3 publications

References 18 publications

17 beta-estradiol acts directly on the clonal osteoblastic cell line UMR106.

17 beta-estradiol acts directly on the clonal osteoblastic cell line UMR106.

The Solanum glaucophyllum Desf. extract reduces mineralized matrix synthesis in osteogenically differentiated rat mesenchymal stem cells in vitro

Phosphorus utilization in domestic animals

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