Influence of pathological progression on the balance between cellular and humoral immune responses in bovine tuberculosis

Welsh, Michael; Cunningham, Rodat T.; Corbett, David; Girvin, R. Martyn; McNair, J.; Skuce, Robin; Bryson, D.G.; Pollock, J.M.

doi:10.1111/j.1365-2567.2004.02003.x

Cited by 170 publications

(147 citation statements)

References 50 publications

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“…Activation of NF-κB in macrophages induces the production of proinflammatory cytokines IL-2, IL-6 [3] which may be involved in granuloma formation. Besides, mycobacterial specific IgG1 antibody responses and production of high levels of IFN-γ (Th0 profile) were associated with increased pathogenesis in bovine tuberculosis [42]. Thus, we suggest an active role of bovine IgG1 in paratuberculosis infection through bacteria-macrophage association.…”

Section: Discussionmentioning

confidence: 89%

Bovine IgG1 antibodies againstMycobacterium aviumsubsp.paratuberculosisprotein p34-cx improve association of bacteria and macrophages

et al. 2007

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-Paratuberculosis, caused by Mycobacterium avium subsp. paratuberculosis (Map), is a chronic granulomatous enteric disease in cattle. Among molecular components of Map, protein p34 was identified as specific and immunodominant for bovine B cells. In order to determine if specific antibodies could influence the course of Map pathogenesis, the interaction between bacteria and bovine macrophages was studied. Bovine polyclonal antibodies from 3 calves vaccinated with protein p34-cx, 6 calves vaccinated with heatkilled Map, 8 naturally infected, and 3 healthy calves -as negative controls-were used. Specific anti-Map, -p34-cx and -PPA-3 antibodies were evaluated and isotype characterized. Infected and Map vaccinated animals showed similar IgG1 and IgG2 response against Map whole bacteria. When p34-cx was used as the antigen, mainly IgG1 and IgG3 were detected in infected and only IgG1 in p34-cx vaccinated animals. Bovine polyclonal antibodies from three animals of each category were isolated and affinity purified through Map and p34-cx columns. The effect of these antibodies in association with Map and a transformed bovine peritoneal macrophage's cell line (Bov-Mac) as well as activation of NF-κB transcription factor was studied. Our results show that association of Map significantly increased in vitro after pretreatment with bovine anti-Map or anti-p34-cx antibodies obtained from vaccinated or infected cattle when compared with those of controls. Improved activation of NF-κB was detected in macrophages that ingested Map opsonized with either anti-Map or anti-p34-cx specific antibodies of infected or vaccinated calves, suggesting that both anti-Map and IgG1 anti-p34-cx antibodies support Map-macrophage interactions.Mycobacterium avium subsp. paratuberculosis / p34 / IgG1 / macrophages / cattle

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Section: Discussionmentioning

confidence: 89%

Bovine IgG1 antibodies againstMycobacterium aviumsubsp.paratuberculosisprotein p34-cx improve association of bacteria and macrophages

et al. 2007

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“…Most of these antigens have achieved a sensitivity and specificity of around 90%, and their recommendations are based on the existence of anergic animals, as well as increased antibody titres in more advanced stages of the disease (Faye et al, 2011;McNair et al, 2001;Welsh et al, 2005).…”

Section: Enzyme-linked Immunosorbent Assays (Elisa)mentioning

confidence: 99%

Diagnosis of bovine tuberculosis: review of main techniques

2015

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Bovine tuberculosis (BTB) remains an important economic and zoonotic problem in Latin America. Traditionally, the fight against BTB is initiated by the implementation of routine diagnostic tests for certification of free properties. The diagnosis of BTB can be made by direct and indirect methods, in which we can mention clinical, post mortem, histopathological, immunological, bacteriological and molecular methods. The renewal of scientific interest in tuberculosis in recent year has led to develop and improve methods of diagnosis, prevention, control and eradication of BTB. The aim of this review is to present and discuss different diagnosis methods of BTB.Keywords: tuberculosis bovine, diagnosis, Mycobacterium bovis, new techniques. Diagnóstico de tuberculose bovina: revisão das principais técnicas ResumoA tuberculose bovina (BTB) continua sendo um importante problema econômico na América Latina, com potenciais consequências zoonóticas. Tradicionalmente, a luta contra a tuberculose bovina tem sido iniciada pela execução de testes de diagnóstico de rotina para a certificação de propriedades livres da doença. O diagnóstico de BTB pode ser feito através de métodos diretos e indiretos, nos quais podemos citar os métodos clínicos, post mortem, histopatológicos, imunológicos, bacteriológicos e moleculares. A renovação do interesse científico em tuberculose nos últimos anos tem levado à necessidade de desenvolver e melhorar os métodos de diagnóstico, prevenção, controle e erradicação da BTB. O objetivo deste artigo é analisar e discutir sobre os diferentes métodos de diagnóstico de BTB.Palavras-chave: tuberculose bovina, diagnóstico, Mycobacterium bovis, novas técnicas.

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“…25 A reciprocal relationship has been documented between the host T-cell responses and the extent of disease during mycobacterial infections. 12,14,29 Studies of human patients infected with M. tuberculosis suggest a predominance of Th1-mediated immunity in the early stages of disease, with a shift to Th2-mediated immunity in the more advanced stages. 5 This shift in host immunity has also been observed for cows infected with M. paratuberculosis, with strong IFN-c responses noted in the early stages of disease, followed by an upregulation of IL-10 in the latter stages.…”

Section: Introductionmentioning

confidence: 99%

Augmentation of Secreted and Intracellular Gamma Interferon following Johnin Purified Protein Derivative Sensitization of Cows Naturally Infected withMycobacterium AviumSubsp.Paratuberculosis

Stabel

Kimura²,

Robbe‐Austerman

2007

J VET Diagn Invest

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Abstract. Measurement of secreted interferon (IFN)-c has proven to be a valuable tool for the detection of animals infected with mycobacterial pathogens, including Mycobacterium avium subsp. paratuberculosis. Previous reports have suggested that tuberculin skin testing can influence the performance of the IFN-c assay. In the present study, healthy noninfected cows, and cows subclinically and clinically infected with M. paratuberculosis were administered an intradermal injection of johnin purified protein derivative (JPPD) and effects on secreted and intracellular IFN-c were observed. Intradermal injection resulted in significant increases in secreted IFN-c for subclinically infected cows after stimulation of peripheral blood mononuclear cells (PBMC) with concanavalin A or M. paratuberculosis antigen preparations (whole-cell sonicate and JPPD) on days 7 and 10 postinjection. Intracellular IFN-c was increased after intradermal injection in total PBMC for all treatment groups and was higher within CD4+ and CD8+ subpopulations for infected cows compared to healthy controls throughout the study. When T-cell populations were further defined by CD45RO expression, intracellular IFN-c was higher within CD8+/CD45RO+ lymphocytes compared to CD4+/CD45RO+ cells for subclinically and clinically infected cows but similar within these subpopulations for healthy controls. These results indicate that intradermal sensitization of cows in the subclinical stage of infection will upregulate expression of IFN-c, enhancing the sensitivity of this assay. In addition, CD8+ lymphocytes appear to play an important role as a mediator of M. paratuberculosis infection in naturally exposed cattle.

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Influence of pathological progression on the balance between cellular and humoral immune responses in bovine tuberculosis

Cited by 170 publications

References 50 publications

Bovine IgG1 antibodies againstMycobacterium aviumsubsp.paratuberculosisprotein p34-cx improve association of bacteria and macrophages

Bovine IgG1 antibodies againstMycobacterium aviumsubsp.paratuberculosisprotein p34-cx improve association of bacteria and macrophages

Diagnosis of bovine tuberculosis: review of main techniques

Augmentation of Secreted and Intracellular Gamma Interferon following Johnin Purified Protein Derivative Sensitization of Cows Naturally Infected withMycobacterium AviumSubsp.Paratuberculosis

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