Influence of pelleting on microbiological and mycotoxical correctness of feed mixtures with bentonite supplement

Abstract

Influence of pelleting calf feed mixtures supplemented with bentoni on microbiological and mycotoxicological properties was investigated. Microbiological and mycotoxicological quality was investigated at the production day (day 0) and after 45 days of storage. Total count of microorganisms in the pelleted mixture, at the day 0 (280.000/g), was several times lower than in the powdered mixture (2.000.000/g). Similar results were obtained at day 45 when the total number of microorganisms in the pelleted mixture was 270.000/g and 1.800.000/g in the powdered mixture. Number of yeasts and molds at the production day in the pelleted mixture was 650/g, and in the powdered mixture it was 27.000/g. Similar results were obtained 45 days later when the number of yeasts and molds in the pelleted mixture was 540/g, and 16.000/g in the powdered mixture. There were 6 species identified in the pelleted mixture, and 9 species in the powdered mixture at the day of production. Similar mold species ratio in the pelleted (11) and powdered mixture (13) was found at day 45. In the examined samples representatives of Fusarium genus - F. subglutinans i F. verticillioides dominated. Number of sultite-redzcing clostridia in the mixtures, in both observed periods, was similar (below 1000/g of sample). By mycotoxicological analysis of mixtures at the production day, only trichotecene (T-2 toxin) presence was found in amount of 0,337 mg/kg. The applied technological procedure of pelleting with bentonite supplement, had positive influence on the improvement of microbiological and toxicological properties of mixture