Influence of periodontal condition on levels of human beta defensins 1 and 2 in saliva

Abstract

Many research studies have looked into periodontal disease biomarkers. The aim of this study was to assess salivary levels of human beta defensin 1 and 2 (hBD-1) and (hBD-2) in different periodontal condition. The Periodontal Screening & Recording (PSR) system, a modification of de Community Periodontal Index Treatment Needs (CPITN), was used to evaluate the periodontal treatment needs of 120 patients without any systemic disease. Salivary levels of hBD-1 and hBD-2 were determined by ELISA. Higher hBD-1 levels were detected in the saliva of patients with a PSR/CPITN index of 3 and 4 (7,363±2,837pg/ mL) and in those with a PSR/CPITN index of 1 and 2 (7,750±3,477pg/mL) when compared to patients without periodontal disease, with a PSR/CPITN index of 0 (5,644±2,747pg/ mL; P=0.013). As with hBD-1, hBD2 levels were also higher in the saliva of patients with a PSR/CPITN index of 3 and 4 (310±98pg/mL) and a PSR/CPITN index of 1 and 2 (318±85pg/mL) when compared to patients with a PSR/CPITN index of 0 (251±87pg/mL; P=0.011). In conclusion, compared to patients without periodontal disease, PSR/CPITN index 0, we verified higher salivary concentrations of hBD-1 and hBD-2 in patients with some degree of periodontal detected by PSR/CPITN between 1 and 4.

Oral Cavity Beta-Defensin Levels Are Regulated Differently during Radiotherapy in Head and Neck Cancer Patients

“…The quantitative detection of hBDs in oral fluids has been applied in various oral conditions, and the loss of hBD is not routinely encountered [41,[50][51][52][53][54][55]. Studies have shown that individuals with periodontitis could have higher levels of hBDs in their saliva compared to individuals without periodontitis [52][53][54]56,57]. Additionally, research has also shown that there is a positive correlation between the severity of periodontitis in diabetic individuals and hBD levels, meaning that as the severity of periodontitis increases, so do hBD levels [56,57].…”

“…Studies have shown that individuals with periodontitis could have higher levels of hBDs in their saliva compared to individuals without periodontitis [52][53][54]56,57]. Additionally, research has also shown that there is a positive correlation between the severity of periodontitis in diabetic individuals and hBD levels, meaning that as the severity of periodontitis increases, so do hBD levels [56,57]. Pereira et al found that patients with periodontitis displayed elevated levels of HBD-2 and hBD-3 in their oral fluids compared to healthy individuals [52,53].…”