Influence of pH upon the activity of glycosidases and proteinases of intestinal mucosa, chyme and microbiota in fish

Кузьмина, В. В.; Skvortsova, E. G.; Zolotareva, G. V.; Sheptitskiy, V. A.

doi:10.1007/s10695-010-9426-3

Cited by 17 publications

(8 citation statements)

References 26 publications

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“…It is worth noting that the characters of pH dependence of TAA in the whole organism of invertebrates and associated micro biota are similar in most of the studied species. The data given in the present paper accord well with the results of the studies on the effect of pH on TAA in the fish enteral microbiota [28].…”

Section: Discussionsupporting

confidence: 90%

“…As a rule at these studies the enzyme activities were deter mined at several values of pH. As was shown in the study [28] on the pH dependence of proteinases in the intestine mucosa, chyme, and enteral microbiota of piscivorous fish, the maxima of the activities of studied preparations do not always coincide. The differences in the characteristics of proteinases of enteral micro biota were most pronounced.…”

Section: Introductionmentioning

confidence: 99%

“…The differences in the characteristics of proteinases of enteral micro biota were most pronounced. The maximal activity of the proteinases in the intestine and chyme mucosa was observed at pH 10, while the maximal activity in the enteral microbiota was observed at the zone of alka line, neutral, and even acid values [7,28].…”

Section: Introductionmentioning

confidence: 99%

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The in vitro effect of pH on glycosidase activity in potential preys of planktivorous and benthivorous fish

2015

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It is shown that the level of total amylolytic activity (TAA) in the whole organism of potential preys (belonging to various taxonomic groups) of planktivorous and benthivorous fishes, as well as in the associated microbiota, depends largely on pH values in a medium. Maximal values of TAA in all species of invertebrates and in the micro flora isolated from their bodies are observed at pH 7.0; minimal ones are found at pH 10.0. The microbiota of Dreissena and of crustacean plankton may efficiently depolymerize polysaccharides within the zone of acid pH values.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

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The in vitro effect of pH on glycosidase activity in potential preys of planktivorous and benthivorous fish

2015

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“…However, n = 3 is not unusual to use when evaluating gut microbiota (e.g. Hagi, Tanaka, Iwamura & Hoshino ; Zhou, Liu, Shi, He, Yao & Ringø ; Kuz‘mina, Skvortsova, Zolotareva & Sheptitskiy ). We suggest that the sequenced bacteria probably belong to the autochthonous gut microbiota as both the PI and DI were flushed three times in sterile saline solution (0.9% NaCl) prior to sampling.…”

Section: Discussionmentioning

confidence: 99%

The effect of dietary chitin on the autochthonous gut bacteria of Atlantic cod (Gadus morhuaL.)

Zhou

Karlsen

et al. 2012

Aquac Res

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In the present study the impact on autochthonous (adherent) bacteria in proximal intestine (PI) and distal intestine (DI) of Atlantic cod (Gadus morhua L.) was evaluated following feeding of a control diet and a diet supplemented with 5% chitin. The autochthonous gut bacteria were investigated using denaturing gradient gel electrophoresis (DGGE). Analysis of the microbiota associated with PI and DI of Atlantic cod indicate that dietary chitin modulate the intestinal bacterial community. For example, band 25 (Escherichia coli-like), band 14 (Anaerorhabdus furcosa-like) and band 29 (uncultured bacterium-like) in PI were depressed by dietary chitin (P < 0.05). The number of bands (23.7 ± 5.4) in DI of fish fed chitin was marginally higher than the control fish (16.7 ± 2.1) (P = 0.065), and the relative abundances of band 6 (swine faecal bacterium-like) were marginally stimulated by dietary chitin (P = 0.095). Furthermore, the present study reports several novel sequences not previously reported in the gastrointestinal tract of Atlantic cod. Whether the dietary effect of chitin on gut bacterial community has any positive effect of fish health merits further investigations.

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“…Relevant information regarding enzymatic activity and the digestive processes from each fish species is essential for developing correct feeding formulations (López-López et al, 2005). Therein, digestive enzyme patterns are correlated to fish feeding habits and digestive capacity (Kuz'mina et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Characterization of digestive enzymes from captive Brazilian flounder Paralichthys orbignyanus

et al. 2017

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Knowledge of specific enzyme activity, along with animal habits and digestive capacity is essential in formulating an appropriate diet for any species. In this study, we evaluated and characterized the activity of digestive enzymes present in the liver, intestine, and stomach of Paralichthys orbignyanus. The effects of pH and temperature on enzyme activity were also evaluated via the use of specific substrates. The use of specific substrates and inhibitors showed strong evidence of the presence of trypsin (BApNA= 0.51 ± 0.2 mU mg -1 ), chimotrypsin (SApNA= 2.62 ± 1.8 mU mg -1 ), and aminopeptidases (Leu-p-Nan =0.9709 ± 0.83 mU mg -1 ) in the intestine. Optimum pH for the activity of trypsin, chemotrypsin, leucino aminopeptidase, amilase, and pepsin were 9.5, 9.0, 8.0, 7.5, and 3.5, respectively, while optimum temperatures were 50, 50, 50, 40, and 45 °C, respectively. These results provide additional information regarding the biology of Brazilian flounder and can be used as a basis for further studies regarding fish feeding physiology.Keywords: trypsin, protease, digestive tract. Caracterização de enzimas digestivas do linguado brasileiro de cativeiro Paralichthys orbignyanusResumo O conhecimento da atividade enzimática é essencial para formular uma correta dieta específica para espécie, além de estarem correlacionadas com o hábito da alimentação e capacidade digestive. Neste estudo determinamos e caracterizamos a atividade enzimática presente no intestino, estômago e fígado do linguado Paralichthys orbignyanus. Os efeitos da temperatura e pH sobre a atividade enzimática também foram avaliados utilizando substratos específicos. O uso de substratos e inibidores específicos mostrou uma forte evidência da presença da tripsina (BApNA = 0,51 ± 0,2 mU mg-1), quimotripsina (SAPNA = 2,62 ± 1,8 mU mg-1), e as aminopeptidases (Leu-p-Nan = 0,97 ± 0,83 mU mg-1) no intestino. O pH ótimo observado para a atividade de tripsina, quimotripsina, leucino aminopeptidase, amilase e pepsina foi 9,5, 9,0, 8,0, 7,5 e 3,5, respectivamente. A temperatura ótima observada foi 50, 50, 50, 40 e 45 °C, respectivamente. Estes resultados fornecem informações adicionais sobre a biologia do linguado brasileiro e pode ser usado como base para novos estudos sobre fisiologia alimentar.Palavras-chave: tripsina, proteases, trato digestório.Candiotto, F.B. et al.

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Influence of pH upon the activity of glycosidases and proteinases of intestinal mucosa, chyme and microbiota in fish

Cited by 17 publications

References 26 publications

The in vitro effect of pH on glycosidase activity in potential preys of planktivorous and benthivorous fish

The in vitro effect of pH on glycosidase activity in potential preys of planktivorous and benthivorous fish

The effect of dietary chitin on the autochthonous gut bacteria of Atlantic cod (Gadus morhuaL.)

Characterization of digestive enzymes from captive Brazilian flounder Paralichthys orbignyanus

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