“…The current study is a human observational study and it has conformed to the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines. Soft tissue debris on teeth was cleaned and inspected under a polarized light microscope (magnification x25) for cracks and white spot lesions, followed by disinfection in 5.25% sodium hypochlorite solution for one hour and then kept in 0.1% thymol solution at 4°C until the time of use [8,13].…”