Influence of physiological cell stages on biofilm formation by Bacillus cereus of dairy origin

Pagedar, Ankita; Singh, Jasbir

doi:10.1016/j.idairyj.2011.10.009

Cited by 29 publications

(17 citation statements)

References 39 publications

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“…ESEM pictures confirmed recent data concerning biofilm formation in mesophilic and thermophilic sporeforming bacteria (Gopal et al, 2015;Majed et al, 2016). Bacillus biofilms were previously shown to contain both spores and vegetative populations (Lindsay et al, 2006;Pagedar and Singh, 2012). In addition, sporulation and biofilm formation have been shown to occur simultaneously in the thermophilic bacilli Anoxybacillus flavithermus (Burgess et al, 2010).…”

Section: Discussionsupporting

confidence: 80%

Evaluation of a non-submerged cultivation assay combined to ESEM imaging for analysis of biofilms formed by dairy-associated sporeforming bacteria

Malek¹

2016

Afr. J. Microbiol. Res.

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In the dairy industry, the biofilms formed by spore forming bacteria are not well characterized. Microscopic methods are crucial for the study of biofilm structural and architectural features. Here, a simple surface-associated non-submerged model combined to environmental scanning electron microscope (ESEM) imaging was used for the study of Bacillus cereus and Geobacillus spp. dairy biofilms. To evaluate the utility of this approach, non-submerged biofilms were compared to those developed in situ on stainless steel coupons introduced inside milk processing lines. Results reveal that both B. cereus and Geobacillus spp. are able to form specific biofilm characteristics on nonimmersed surfaces, notably an original dispersion style not previously described. Non-submerged biofilms in vitro are elaborate three-dimensional or extensive complex structures well resolved in ESEM and comparable to dairy biofilms in situ. The non-submerged surface-associated biofilm combined to ESEM imaging revealed a relevant model for the study of dairy biofilms.

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Section: Discussionsupporting

confidence: 80%

Evaluation of a non-submerged cultivation assay combined to ESEM imaging for analysis of biofilms formed by dairy-associated sporeforming bacteria

Malek¹

2016

Afr. J. Microbiol. Res.

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“…This microorganism is present in the milk submitted to UHT treatment may be due to some factors: bacterial spores resistant to heat treatment that were present in the raw milk, the improper packaging of milk after heat treatment allowing the entrance of microorganisms, recontamination after heat treatment (SALUSTIANO et al, 2009), whereas the spores of Bacillus cereus can form biofilm (PAGEDAR & SINGH, 2012).…”

Section: Introductionmentioning

confidence: 99%

Detection of Bacillus cereus isolated during ultra high temperature milk production flowchart through random amplified polymorphic DNA polymerase chain reaction

et al. 2015

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“…There are several reports on the capacity of diverse members of the Bacillus genus, particularly B. cereus and B. subtilis, to form biofilms in in vitro models [6,27]. Bacterial adhesion to a surface is an essential step for the formation of biofilms [19].…”

Section: Discussionmentioning

confidence: 99%

“…We selected to use the Bt spores suspension to perform both the adhesion and the biofilms formation assays in this work based on the findings [27], as they observed a greater efficiency in adhesion and biofilms formation of B. cereus spores, in contrast to planktonic and/or sessile cells. Besides, they also observed that the sporulated phase of growth presents a more prolonged lag phase in the growth curve in biofilms than in suspension, which could have implications in the resistance of biofilms to stressing factors.…”

Section: Adhesion Capacity Of Bacillus Thuringiensis Spores and Its Rmentioning

confidence: 99%

“…According to Auger et al [25], less than 50% of the B. cereus and Bt strains showed capacity to form biofilm in their assays. Formation of biofilms has been related also to the resistance of these species to diverse antibiotics and disinfectants [26,27] found that the latent spores of B. cereus were more efficient in starting and forming biofilms as compared to bacterial cells in vegetative or sessile state. These same authors evidenced that the production of biofilm in cultures with spores depends on the growth curves, which does not occur in cultures in suspension, and could be related to the resistance of the biofilm to adverse factors.…”

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confidence: 99%

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Adhesion Capacity of Bacillus thuringiensis Spores and its Relation with Biofilm Formation

León¹

2015

SOJMID

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Open Access Research Articlehaving an attenuated immuneresponse [7].It is known that Bt spores and those of other Bacillus species, presenting exosporium, have a higher hydrophobicity, conferring them a higher adhesive potential to diverse materials [8,9], such as those used in industrial food processing [1,[10][11][12][13]. To this respect, there are several reports on the in vitro capacity of these bacteria to adhere to stainless steel laminae and/or synthetic polymers [14][15][16][17][18].Bacterial adhesion to a surface is a two-phases process: in the first phase, planktonic bacteria are moved to a surface by the effect of physical forces such as Brownian motion, van der Waals attraction forces, gravitational forces, the effect of surface electrostatic charge and hydrophobic interactions; in the second phase of adhesion, molecular irreversible reactions between bacterial surface structures and substratum surfaces become predominant [19,20]. Adhesion is an essential stage for the formation and subsequent growth of biofilms [19,21,22].Biofilms are defined as microbial communities embedded in a polymer layer or matrix adhered to an inert surface or substrate, or to living cells or tissues [23,24]. Several members of the Bacillus genus are capable of forming biofilms in vitro at variable magnitudes dependent on the incubation time [6,25]. According to Auger et al. [25], less than 50% of the B. cereus and Bt strains showed capacity to form biofilm in their assays. Formation of biofilms has been related also to the resistance of these species to diverse antibiotics and disinfectants [26,27] found that the latent spores of B. cereus were more efficient in starting and forming biofilms as compared to bacterial cells in vegetative or sessile state. These same authors evidenced that the production of biofilm in cultures with spores depends on the growth curves, which does not occur in cultures in suspension, and could be related to the resistance of the biofilm to adverse factors.Despite the existence of important collections of Bt isolated from different regions of Mexico, few studies have focused on the possible associations and distribution of features that could contribute to the analysis and a better understanding of the relations at the level of species and subspecies of the very AbstractSpores of Bacillus thuringiensis (Bt), used as bioinsecticide, represent a potential contamination risk of the equipment used for food processing and could also induce potential gastrointestinal symptoms in special cases of susceptible individuals. The objective of this work was to explore the in vitro adhesion capacity of Bt spores and its relation to the ability to form biofilms. The adhering capacity of spores of 65 Bt strains was determined in stainless steel tubes, as well as their ability to form biofilms on polystyrene microplates. We analyzed the relation of these two features. All strains showed a variable adhering capacity to stainless steel, ranging from 0.04% to 1.21% of the initial spore load of ~1.7×10 7 CFU/ mL. Th...

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Influence of physiological cell stages on biofilm formation by Bacillus cereus of dairy origin

Cited by 29 publications

References 39 publications

Evaluation of a non-submerged cultivation assay combined to ESEM imaging for analysis of biofilms formed by dairy-associated sporeforming bacteria

Evaluation of a non-submerged cultivation assay combined to ESEM imaging for analysis of biofilms formed by dairy-associated sporeforming bacteria

Detection of Bacillus cereus isolated during ultra high temperature milk production flowchart through random amplified polymorphic DNA polymerase chain reaction

Adhesion Capacity of Bacillus thuringiensis Spores and its Relation with Biofilm Formation

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