Influence of plant growth regulator and rhizobial inoculation on nodulation and soybean nitrogen contant

Redžepović, Sulejman; Sikora, Sanja; Čolo, Josip; Blažinkov, Mihaela; Pećina, Marija

doi:10.1556/crc.35.2007.2.207

Cited by 4 publications

(2 citation statements)

References 3 publications

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“…Besides, symbiotic characterization of indigenous strains is a prerequisite for selection of the most effi cient strains that are at the same time well-adapted to the soils of central Croatia. Investigations conducted so far in Croatia involved indigenous rhizobial populations that nodulate soybean (12,13), fi eld pea (14) and alfalfa (15). It is assumed that indigenous rhizobial strains with diff erent symbiotic characteristics are present in the soils of central Croatia.…”

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confidence: 99%

Genetic Diversity and Symbiotic Efficiency of Indigenous Common Bean Rhizobia in Croatia

Pohajda¹,

Babić

Rajnović

et al. 2016

Food Technol. Biotechnol.

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Natural process of symbiotic nitrogen fi xation has a unique role in a sustainable legume production. Nodule bacteria, collectively named rhizobia, in symbiotic associations with legumes enable considerable entries of biologically fi xed nitrogen into ecosystems. In order to maxi- SummaryNodule bacteria (rhizobia) in symbiotic associations with legumes enable considerable entries of biologically fi xed nitrogen into soil. Eff orts are therefore made to intensify the natural process of symbiotic nitrogen fi xation by legume inoculation. Studies of fi eld populations of rhizobia open up the possibility to preserve and probably exploit some indigenous strains with hidden symbiotic or ecological potentials. The main aim of the present study is to determine genetic diversity of common bean rhizobia isolated from diff erent fi eld sites in central Croatia and to evaluate their symbiotic effi ciency and compatibility with host plants. The isolation procedure revealed that most soil samples contained no indigenous common bean rhizobia. The results indicate that the cropping history had a signifi cant impact on the presence of indigenous strains. Although all isolates were found to belong to species Rhizobium leguminosarum, signifi cant genetic diversity at the strain level was determined. Application of both random amplifi cation of polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC--PCR) methods resulted in similar grouping of strains. Symbiotic effi ciency of indigenous rhizobia as well as their compatibility with two commonly grown bean varieties were tested in fi eld experiments. Application of indigenous rhizobial strains as inoculants resulted in signifi cantly diff erent values of nodulation, seed yield as well as plant nitrogen and seed protein contents. The most abundant nodulation and the highest plant nitrogen and protein contents were determined in plants inoculated with R. leguminosarum strains S 17/2 and S 21/6 . Although, in general, the inoculation had a positive impact on seed yield, diff erences depending on the applied strain were not determined. The overall results show the high degree of symbiotic effi ciency of the specifi c indigenous strain S 21/6 . These results indicate diff erent symbiotic potential of indigenous strains and confi rmed the importance of rhizobial strain selection. These are the fi rst studies of indigenous common bean rhizobia in Croatia that provide the basis for further characterization and selection of highly effi cient indigenous strains and their potential use in agricultural practice and future research.

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confidence: 99%

Genetic Diversity and Symbiotic Efficiency of Indigenous Common Bean Rhizobia in Croatia

Pohajda¹,

Babić

Rajnović

et al. 2016

Food Technol. Biotechnol.

Self Cite

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“…In addition to their effects on the establishment of symbiotic relationships, T3Es also govern the induction of plant defense responses, thereby influencing rhizobia colonization efficiency [15][16][17]. GmPR1 (pathogenesis-related gene 1) expression was significantly elevated in the roots of Williams 82 soybeans following inoculation with the TtsI mutant strain as compared to the wild type strain [18]. Research focused on pathogenic bacteria such as Pseudomonas and Xanthomonas has additionally demonstrated an essential role for T3Es in the incidence of infection [19,20].…”

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confidence: 99%

NopAA and NopD Signaling Association-Related Gene GmNAC27 Promotes Nodulation in Soybean (Glycine max)

Wang,

Jia,

et al. 2023

IJMS

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Rhizobia secrete effectors that are essential for the effective establishment of their symbiotic interactions with leguminous host plants. However, the signaling pathways governing rhizobial type III effectors have yet to be sufficiently characterized. In the present study, the type III effectors, NopAA and NopD, which perhaps have signaling pathway crosstalk in the regulation of plant defense responses, have been studied together for the first time during nodulation. Initial qRT-PCR experiments were used to explore the impact of NopAA and NopD on marker genes associated with symbiosis and defense responses. The effects of these effectors on nodulation were then assessed by generating bacteria in which both NopAA and NopD were mutated. RNA-sequencing analyses of soybean roots were further utilized to assess signaling crosstalk between NopAA and NopD. NopAA mutant and NopD mutant were both found to repress GmPR1, GmPR2, and GmPR5 expression in these roots. The two mutants also significantly reduced nodules dry weight and the number of nodules and infection threads, although these changes were not significantly different from those observed following inoculation with double-mutant (HH103ΩNopAA&NopD). NopAA and NopD co-mutant inoculation was primarily found to impact the plant–pathogen interaction pathway. Common differentially expressed genes (DEGs) associated with both NopAA and NopD were enriched in the plant–pathogen interaction, plant hormone signal transduction, and MAPK signaling pathways, and no further changes in these common DEGs were noted in response to inoculation with HH103ΩNopAA&NopD. Glyma.13G279900 (GmNAC27) was ultimately identified as being significantly upregulated in the context of HH103ΩNopAA&NopD inoculation, serving as a positive regulator of nodulation. These results provide new insight into the synergistic impact that specific effectors can have on the establishment of symbiosis and the responses of host plant proteins.

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Factors of Soil Genesis of Croatia

Bašić

2013

World Soils Book Series

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Influence of plant growth regulator and rhizobial inoculation on nodulation and soybean nitrogen contant

Cited by 4 publications

References 3 publications

Genetic Diversity and Symbiotic Efficiency of Indigenous Common Bean Rhizobia in Croatia

Genetic Diversity and Symbiotic Efficiency of Indigenous Common Bean Rhizobia in Croatia

NopAA and NopD Signaling Association-Related Gene GmNAC27 Promotes Nodulation in Soybean (Glycine max)

Factors of Soil Genesis of Croatia

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