Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity

Abstract

Bacterial adhesion can be controlled by different material surface properties, such as surface charge, on which we concentrate in our study. We use a silica surface on which poly(allylamine hydrochloride)/sodium poly(4-styrenesulfonate) (PAH/PSS) polyelectrolyte multilayers were formed. The corresponding surface roughness and hydrophobicity were determined by atomic force microscopy and tensiometry. The surface charge was examined by the zeta potential measurements of silica particles covered with polyelectrolyte multilayers, whereby ionic strength and polyelectrolyte concentrations significantly influenced the build-up process. For adhesion experiments, we used the bacterium Pseudomonas aeruginosa. The extent of adhered bacteria on the surface was determined by scanning electron microscopy. The results showed that the extent of adhered bacteria mostly depends on the type of terminating polyelectrolyte layer, since relatively low differences in surface roughness and hydrophobicity were obtained. In the case of polyelectrolyte multilayers terminating with a positively charged layer, bacterial adhesion was more pronounced than in the case when the polyelectrolyte layer was negatively charged.

Polyelectrolyte Multilayers on Silica Surfaces: Effect of Ionic Strength and Sodium Salt Type

“…On the other hand, Wong and coworkers showed that the protein adsorption is drastically lowered on microbicidal hydrophobic/hydrophilic polyelectrolyte multilayers. [18] It was shown recently [19] that that the extent of adhered bacteria mostly depends on the type of terminating polyelectrolyte layer, although surface roughness and hydrophobicity should be also taken into account.…”

A Sticking Point in Assessing Bacterial Contamination: Adhesive Characters of Bacterial Specializations, Swab Features, and Fomite Surface Properties Skew Colony Counts

“…6). This change was necessary as metals are susceptible to surface change during chemical decontamination and bacterial adhesion to metals appears to depend on the terminating polyelectrolyte layer (Kovacevic et al, 2016). Fig.…”

Adhesion of bacteria to wood coatings

“…V literaturi najdemo študije o oprijemu bakterij na najrazličnejše materiale, kot so npr. steklo (Bohinc et al, 2014;Oder et al, 2015), nerjavno jeklo (Fink et al, 2015, silicijev dioksid s plastmi polielektrolita (Kovačević et al, 2016), površine biomateralov (Reid, 1997), ali npr. polietilen tereftalat (Gil-lett et al, 2015).…”

“…Zaradi elektrostatske sile pozitivno nabite materialne kontaktne površine delujejo s privlačno silo na negativno naelektrene bakterijske površine. Nasprotno negativne površine prispevajo odbojno komponento sile na negativno nabite bakterije (Kovačević et al, 2016). (Kapun Dolinar, 2001).…”