Influence of polymerized siloxanes on growth of nosocomial aerobic bacteria

Abstract

Abstract. Health care associated infections are the fourth leading cause of disease in industrialized countries, and the most common complication affecting hospitalized patients. Recently a polymerized siloxane coating substrate was suggested as a promising candidate for the coating of materials which are aimed to be used in cardiovascular tissue engineering. To reduce the risk of tissue remodeling failure after implantation of such engineered tissue implants, coatings substrates for tissue engineering scaffolds might be helpful to lower the risk of bacterial attachment and growth during and after implantation.In the presented study a coating based on polymerized siloxanes was tested with respect to these properties. Tests were performed with aerobic nosocomial bacteria (Escherichia coli and Staphylococcus epidermidis) and an exemplary selection of materials (stainless steel, polycarbonate, soda-lime glass). The siloxane coating was able to significantly reduce bacterial adherence, and it is supposed that this effect is mainly a result of the high hydrophobicity of the coating substrate.

Influence of polymerized siloxane coating on growth and biofilm formation of aerobic grown nosocomial bacteria

“…Surface coating for bacterial adherence and growth reduction [3,[10][11][12]] is a promising approach to address the problem of health care-associated infections (HAI's) which are related to invasive medical devices. A coating based on polymerized siloxanes and establishing a hydrophobic and positively charged surface already proved to reduce the number of adherent aerobic nosocomial bacteria on stainless steel, polycarbonate, and soda-lime glass [4]. The study showed that PDMS also affects the growth rate of aerobic nosocomial bacteria, which were grown planktonically, or in biofilms.…”

“…6630]), and Gram-negative bacteria (Pseudomonas aeruginosa [ATCC strain-no. 27853; LGC, Germany], Escherichia coli-strain ECM1 which was isolated from C57Bl/6N mouse feces and had proved to not contribute to any diarrheagenic pathovar [4]).…”

“…Recently an aqueous emulsion of polydimethylsiloxane (PDMS), which is a widely used silicone oil, was used as a coating substrate on stainless steel, polycarbonate and soda-lime glass. This coating proved to be non-cytotoxic to eukaryotic cells (fibroblasts), but significantly reduced adherence of aerobic nosocomial bacteria (Escherichia coli and Staphylococcus epidermidis) [4]. However, PDMS provides a hydrophobic surface, which is described to be less resistant to bacterial adhesion than hydrophilic surfaces [5,6].…”