Influence of pore sizes in 3D-scaffolds on mechanical properties of scaffolds and survival, distribution, and proliferation of human chondrocytes

Abpeikar, Zahra; Milan, Peiman Brouki; Moradi, Lida; Anjomshoa, Maryam; Asadpour, Shiva

doi:10.1080/15376494.2021.1943077

Cited by 22 publications

(19 citation statements)

References 59 publications

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“…Moreover, we were able to observe cell penetration within the scaffold through all the time points, meaning that pore size and microenvironment was suitable not only to grow but to migrate and populate the whole scaffold. Previous studies noted that scaffold pore size and fiber diameter is highly important for cell population and communication [25,44]. High porous scaffolds improve new tissue development and facilitate effective exchange of nutrients and metabolic waste.…”

Section: Discussionmentioning

confidence: 99%

“…Bone-marrow-derived or other mesenchymal stromal cells (MSC), musclederived stem cells (MDSCs), induced pluripotent stem cells (iPSC), or other stem cells have been used in articular cartilage regeneration process [4,23,24]. Chondrocyte-scaffold constructs are being investigated as well [25]. Nevertheless, various seeding densities, cell phenotypes, or passages can result in distinct sometimes contradictory results [26].…”

Section: Introductionmentioning

confidence: 99%

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Functionalized Electrospun Scaffold–Human-Muscle-Derived Stem Cell Construct Promotes In Vivo Neocartilage Formation

et al. 2022

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Polycaprolactone (PCL) is a non-cytotoxic, completely biodegradable biomaterial, ideal for cartilage tissue engineering. Despite drawbacks such as low hydrophilicity and lack of functional groups necessary for incorporating growth factors, it provides a proper environment for different cells, including stem cells. In our study, we aimed to improve properties of scaffolds for better cell adherence and cartilage regeneration. Thus, electrospun PCL–scaffolds were functionalized with ozone and loaded with TGF-β3. Together, human-muscle-derived stem cells (hMDSCs) were isolated and assessed for their phenotype and potential to differentiate into specific lineages. Then, hMDSCs were seeded on ozonated (O) and non-ozonated (“naïve” (NO)) scaffolds with or without protein and submitted for in vitro and in vivo experiments. In vitro studies showed that hMDSC and control cells (human chondrocyte) could be tracked for at least 14 days. We observed better proliferation of hMDSCs in O scaffolds compared to NO scaffolds from day 7 to day 28. Protein analysis revealed slightly higher expression of type II collagen (Coll2) on O scaffolds compared to NO on days 21 and 28. We detected more pronounced formation of glycosaminoglycans in the O scaffolds containing TGF-β3 and hMDSC compared to NO and scaffolds without TGF-β3 in in vivo animal experiments. Coll2-positive extracellular matrix was observed within O and NO scaffolds containing TGF-β3 and hMDSC for up to 8 weeks after implantation. These findings suggest that ozone-treated, TGF-β3-loaded scaffold with hMDSC is a promising tool in neocartilage formation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Functionalized Electrospun Scaffold–Human-Muscle-Derived Stem Cell Construct Promotes In Vivo Neocartilage Formation

et al. 2022

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“…Cells and their microenvironment are highly important in the regenerative process. First, such elements as chemical properties, technical composition, and mechanics are associated with cell behavior, e.g., their migration, proliferation, or differentiation ( Demoor et al, 2014 ; Girão et al, 2020 ; Abpeikar et al, 2021 ). For our scaffold production, we selected PCL, a highly biodegradable, elastic, and low cytotoxic material used in the electrospinning process, which provided a porous structure for our scaffolds.…”

Section: Discussionmentioning

confidence: 99%

Cartilage regeneration using improved surface electrospun bilayer polycaprolactone scaffolds loaded with transforming growth factor-beta 3 and rabbit muscle-derived stem cells

Malinauskas

Jankauskaitė²,

Aukstikalne³

et al. 2022

Front. Bioeng. Biotechnol.

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Polycaprolactone (PCL) has recently received significant attention due to its mechanical strength, low immunogenicity, elasticity, and biodegradability. Therefore, it is perfectly suitable for cartilage tissue engineering. PCL is relatively hydrophobic in nature, so its hydrophilicity needs to be enhanced before its use in scaffolding. In our study, first, we aimed to improve the hydrophilicity properties after the network of the bilayer scaffold was formed by electrospinning. Electrospun bilayer PCL scaffolds were treated with ozone and further loaded with transforming growth factor-beta 3 (TGFβ3). In vitro studies were performed to determine the rabbit muscle-derived stem cells’ (rMDSCs) potential to differentiate into chondrocytes after the cells were seeded onto the scaffolds. Statistically significant results indicated that ozonated (O) scaffolds create a better environment for rMDSCs because collagen-II (Coll2) concentrations at day 21 were higher than non-ozonated (NO) scaffolds. In in vivo studies, we aimed to determine the cartilage regeneration outcomes by macroscopical and microscopical/histological evaluations at 3- and 6-month time-points. The Oswestry Arthroscopy Score (OAS) was the highest at both mentioned time-points using the scaffold loaded with TGFβ3 and rMDSCs. Evaluation of cartilage electromechanical quantitative parameters (QPs) showed significantly better results in cell-treated scaffolds at both 3 and 6 months. Safranin O staining indicated similar results as in macroscopical evaluations—cell-treated scaffolds revealed greater staining with safranin, although an empty defect also showed better results than non-cell-treated scaffolds. The scaffold with chondrocytes represented the best score when the scaffolds were evaluated with the Mankin histological grading scale. However, as in previous in vivo evaluations, cell-treated scaffolds showed better results than non-cell-treated scaffolds. In conclusion, we have investigated that an ozone-treated scaffold containing TGFβ3 with rMDSC is a proper combination and could be a promising scaffold for cartilage regeneration.

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“…3c and d). 108 Collagen porous scaffolds prepared with ice particulates in the range of 150-250 mm showed the highest promoting effect on the gene expression and the production of cartilaginous matrix proteins, as well as on cartilage regeneration. 113 Among all the materials tested for scaffold fabrication, polymers are widely used can be mainly categorized as natural and synthetic polymers.…”

Section: Scaffoldsmentioning

confidence: 94%

The potential of graphene coatings as neural interfaces

Lopes,

Moreira,

Bramini

et al. 2024

Nanoscale Horiz.

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Influence of pore sizes in 3D-scaffolds on mechanical properties of scaffolds and survival, distribution, and proliferation of human chondrocytes

Cited by 22 publications

References 59 publications

Functionalized Electrospun Scaffold–Human-Muscle-Derived Stem Cell Construct Promotes In Vivo Neocartilage Formation

Functionalized Electrospun Scaffold–Human-Muscle-Derived Stem Cell Construct Promotes In Vivo Neocartilage Formation

Cartilage regeneration using improved surface electrospun bilayer polycaprolactone scaffolds loaded with transforming growth factor-beta 3 and rabbit muscle-derived stem cells

The potential of graphene coatings as neural interfaces

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