The effects of C2H4 concentration, duration and timing of exposure to C2H4, and temperature on storage performance of kiwifruit (Actinidia chinensis Planch. ‘Hayward’) kept in air or a controlled atmosphere (CA) of 2% O2 + 5% CO2 were investigated. The presence of 0.05, 0.1, 0.5, 1.0, or 5.0 μl·liter−1 C2H4 in CA accelerated softening and induced white core inclusions (WCI) which increased with storage time and C2H4 concentration. There was no difference between a 2-week or a 4-week exposure to 0.5 μl·liter−1 C2H4 at the beginning of CA storage in the extent of softening or WCI incidence and severity, but prolonged exposures accelerated deterioration. The softening rate of kiwifruit kept in air or CA increased with temperature. The incidence and severity of WCI were much greater in fruit kept in CA + 0.5 μl·liter−1 C2H4 at 0° or 2.5°C than in fruit stored at 5° or 10°.