Influence of primaquine and ritonavir interaction on CYP3A4 mRNA expression in HepG2 cell culture

Iskandarmudasyah, Adam; Louisa, Melva; Arleni, Arleni; Jusman, Sri Widia A.; Suyatna, Franciscus D.

doi:10.13181/mji.v21i1.471

Cited by 4 publications

(4 citation statements)

References 15 publications

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“…In vitro, HepG2 cells can produce CYP3A4 enzymes known to metabolize more than 50% of drugs. 59,60 Madin-Darby canine kidney (MDCK) cells are known to be widely used for drug elimination, permeability, and drug solubility tests in vitro, so it is hoped that MO extracts will have a low toxicity effect. 61,62 Our study showed that the toxicity dose of ethanol extract MO was 6-7 times higher than the antioxidant dose in HepG2 cells and 8 times in MDCK cells.…”

Section: Discussionmentioning

confidence: 99%

Impact of Ultrasonic Assisted Extraction on the Protein Yield from Moringa Oleifera Lam Leaves and its Functional Characterization

Kambuno,

Louisa,

Wuyung

et al. 2023

Preprint

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Section: Discussionmentioning

confidence: 99%

Impact of Ultrasonic Assisted Extraction on the Protein Yield from Moringa Oleifera Lam Leaves and its Functional Characterization

Kambuno,

Louisa,

Wuyung

et al. 2023

Preprint

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“…cDNA was synthesized by reverse transcription of each RNA sample using ReverTra Ace® qPCR RT Master Mix (Toyobo Co., Ltd, Tokyo, Japan), according to the manufacturer's instructions. The quantitative evaluation of mRNA expression was carried out by real‐time PCR with the cDNA samples using SsoFast EvaGreen Supermix (Bio‐Rad Laboratories, Inc., CA) with the gene specific primers as follows: forward and reverse primers, Mdr1a were 5′‐TGA ACT GTG ACC ATG CGA GAT GTT AAA TA‐3′ and 5′‐GTC TCT GAA GAC TCT AAA ATG GAC TAA ATG‐3′ (153 bp ) (Higashi et al, ); Bcrp, 5′‐CAA TGG GAT CAT GAA ACC TG‐3′ and 5′‐GAG GCT GGT GAA TGG AGA A‐3′ (536 bp ) (Shibayama et al, ); Pept1 , 5′‐CAC AGC GCC AGC AAC TAT CA‐3′ and 5′‐GAT ATT ACC GAT GGC CAC GG‐3′ (360 bp ) (Naruhashi, Sai, Tamai, Suzuki, & Tsuji, ); β‐actin , 5′‐TCA GCA AGC AGG AGT ATG‐3′ and 5′‐GTC AAG AAA GGG TGT AAC G‐3′ (97 bp ) (Iskandarmudasyah, Louisa, Arleni, Jusman, & Suyatna, ). Real‐time PCR was performed using a thermal cycler CFX96TM Real‐TimeSystem (Bio‐Rad Laboratories, Inc.).…”

Section: Methodsmentioning

confidence: 99%

5‐Fluorouracil treatment alters the expression of intestinal transporters in rats

Yotsumoto

Akiyoshi

Wada

et al. 2017

Biopharm & Drug Disp

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5-Fluorouracil (5-FU), an anticancer drug, causes severe gastrointestinal damage, which may affect the absorption of orally administered drugs including the substrates of intestinal uptake and efflux transporters. This study aimed to investigate quantitatively the effect of 5-FU-induced intestinal damage on the expression of intestinal transporters: P-glycoprotein (P-gp), breast cancer resistance protein (BCRP) and peptide transporter 1 (PEPT1) in rats. The rats were treated with 5-FU (30 mg/kg/day, p.o.) for 5 days to induce intestinal damage, and then the upper, middle and lower intestinal segments were removed. The mRNA and protein expression levels of these transporters in each segment were determined using quantitative real-time PCR and Western blotting, respectively. In the 5-FU-treated rats, the protein levels of P-gp and Bcrp in the upper segment were significantly increased to 15- and 2.6-fold of the control, respectively, while those in other segments were unaffected. Pept1 expression was increased by 5-FU in almost all segments. A remarkable increase in P-gp expression was shown, the uptake of digoxin, a P-gp substrate, in each intestinal segment was measured using a rat everted sac. As a result, the uptake of digoxin in the upper segments of 5-FU-treated rats was decreased compared with that of the control. In conclusion, 5-FU-induced intestinal damage was shown to alter the expression of these transporters, especially in the upper intestinal segment, while the characteristics of the influence varied among the transporters. The 5-FU-induced intestinal damage may affect transporter-mediated drug absorption of orally administered drugs in the clinical setting.

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“…Suspen sel yang telah cair dimasukkan ke dalam medium DMEM yang sebelumnya telah disiapkan. Kultur sel dilakukan pada suhu 37°C dengan CO2 5% [11].…”

Section: Kultur Sel Hepg2unclassified

“…Kultur sel dipindahkan ke dalam tabung kriopreservasi 1,8 mL dan dimasukan ke dalam freezer -80°C selama satu malam. Kemudian kultur sel tersebut dipindahkan ke dalam cryotank berisi nitrogen cair (-196°C) [11].…”

Section: Kultur Sel Hepg2unclassified

Pengaruh Interaksi Ekstrak Etanol Meniran (Phyllanthus niruri L.) dengan Glibenklamid Terhadap Ekspresi gen CYP3A4 pada kultur sel HepG2

2019

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Diabetes Melitus (DM) merupakan suatu sindrom metabolik yang ditandai dengan peningkatan kadar gula (glukosa) darah atau hiperglikemia. Berbagai terapi dilakukan untuk menurunkan hiperglikemia menggunakan obat antidiabetes maupun tanaman herbal. Pada beberapa kasus penggunaan bersamaan obat diabetes oral dan herbal antidiabetes dapat menyebabkan interaksi antara kedua obat tersebut. Penelitian ini bertujuan untuk mengetahui pengaruh interaksi ekstrak etanol meniran dengan glibenklamid terhadap ekspresi gen CYP3A4 pada kultur sel HepG2. Penelitian ini merupakan penelitian eksperimental kuantitatif dengan beberapa variabel, baik variabel independen (kombinasi ekstrak dan glibenklamid), dependen (ekspresi gen CYP3A4). Hasil penelitian ini menunjukkan bahwa pada pemberian glibenklamid tunggal terjadi penurunan ekspresi gen CYP3A4, yaitu pada konsentrasi 100, 50, dan 25 µg/mL sebesar 0,74;0,74;0,94. Selanjutnya pada ekstrak meniran tunggal terjadi peningkatan ekspresi gen CYP3A4 seiring peningkatan konsentrasi ekstrak. Pada kombinasi ekstrak meniran dan glibenklamid terjadi penurunan ekspresi gen seiring dengan peningkatan konsentrasi pengujian. Pemberian kombinasi ekstrak meniran dan glibenklamid menurunkan ekspresi gen CYP3A4 seiring dengan peningkatan konsentrasi pengujian. Penurunan ekspresi gen CYP3A4 berimplikasi pada penurunan metabolisme obat

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Influence of primaquine and ritonavir interaction on CYP3A4 mRNA expression in HepG2 cell culture

Cited by 4 publications

References 15 publications

Impact of Ultrasonic Assisted Extraction on the Protein Yield from Moringa Oleifera Lam Leaves and its Functional Characterization

Impact of Ultrasonic Assisted Extraction on the Protein Yield from Moringa Oleifera Lam Leaves and its Functional Characterization

5‐Fluorouracil treatment alters the expression of intestinal transporters in rats

Pengaruh Interaksi Ekstrak Etanol Meniran (Phyllanthus niruri L.) dengan Glibenklamid Terhadap Ekspresi gen CYP3A4 pada kultur sel HepG2

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