Influence of profilin on sensitisation profiles determined by cutaneous tests and IgE to major allergens in polysensitised patients

Abstract

BackgroundProfilin sensitisation is considered a diagnostic confounding factor in areas where patients are exposed to multiple pollens. The aim of this study is to assess pollen sensitisation profiles in adults and children and to evaluate, by means of component-resolved diagnosis (CRD) and skin prick testing (SPT), which pollens may be considered as risk factors of profilin sensitisation in order to establish the best diagnostic approach in polysensitised patients.MethodsA total of 231 pollen-allergic patients (adults and children) were included, out of the pollen season, from an area with similar levels of pollen exposure. Allergological diagnosis was performed by SPT and determination of specific IgE (sIgE) to major allergen components (ADVIA-Centaur™). Patients had not received immunotherapy in the last 5 years and had to reside in the area for 5 consecutive years before entering the study.ResultsThe relation between sensitisation measured by SPT and by sIgE was studied using a model of cases (patients with +sIgE to a specific allergen) and controls (patients with −sIgE to the same allergen). The outcome, in terms of odds-ratios (OR), was statistically significant for Olea (Ole e 1) (p = 0.0005), Salsola (Sal k 1) (p = 0.0118) and Platanus (Pla a 1+ 2) (p = 0.0372). While positivity of SPT to most pollens was statistically associated with a risk of profilin sensitisation, by CRD the association was statistically significant only for Ole e 1 (OR 3.5, CI 95 %, 1.6–7.6, p = 0.0014), and Phl p 5 (OR 11.9, CI 95 %, 4.1–35.2, p < 0.001). When analysing this association using a logistic regression model, Phl p 5 was the only allergen associated with the risk of being sensitised to profilin (p = 0.0023).ConclusionsIn patients sensitised to profilin, the concordance between SPT and CRD is much lower than in those not sensitised to profilin. CRD is able to provide refined information about which pollens increase the risk of sensitisation to profilin.

Sensitization profile in patients with respiratory allergic diseases: differences between conventional and molecular diagnosis (a cross-sectional study)

“…Consistently with this finding, the multivariate analysis showed that the type of disease significantly contributed to sensitization to group 1 (Der p 1 and Der f 1) house dust mite allergens, whereas the severity of the disease significantly influenced sensitization to group 2 (Der p 2 and Der f 2) house dust mite allergens; sensitization to Lep d 2 was associated with both the type and severity of the disease. Similar relationships have been reported between various pollens and the type of respiratory disease [22, 26, 27]; however, to our knowledge, this is the first study showing a relationship between disease type/severity and house dust mite allergens. Although evidence regarding these specific relationships is still weak, consistent findings that specific antigens are associated with clinical characteristics of the respiratory disease strengthen the importance of using CRD to assess the sensitization profile of patients with respiratory allergy.…”

“…Another limitation of our results was the low prevalence of panallergens found in our study cohort. The presence of these allergens, which is commonly higher in other studies [20, 22, 27], has been considered a key benefit of CRD and may increase the differences between the two types of diagnosis.…”

Precision medicine allergy immunoassay methods for assessing immunoglobulin E sensitization to aeroallergen molecules

“…It also correlated well with the ImmunoCAP ® system. A good agreement and correlation for some allergen components with the ImmunoCAP Immuno Solid-phase Allergen Chip (ISAC) microarray was revealed[ 66 - 68 ].…”

Food allergy in EAACI journals (2016)

“…Profilin sensitization is a diagnostic confounding factor. A study assessed pollen sensitization profiles in adults and children and evaluated these sensitization by means of component‐resolved diagnosis (CRD, ADVIA‐Centaur) and skin prick testing (SPT) . In patients sensitized to profilin, the concordance between SPT and CRD is much lower than in those not sensitized to profilin.…”