Influence of propofol concentration in human plasma on free fraction of the drug

Influence of intralipid on free propofol fraction assayed in human serum albumin solutions and human plasma

“…As earlier outlined, the objective of the present study was to verify if the free propofol fraction increase at a low total drug concentration observed in plasma and HSA solutions spiked with propofol in ethanolic solution [11] occurs when propofol is introduced into protein samples in intralipid emulsion. The run of the relationships between the free propofol fraction and total drug concentration shown in Figure 1 proves that the free propofol fraction increases irrespective of the type of propofol solvent used.…”

“…Hence, it was decided to apply the typical procedure of propofol determination by a simpler and more reliable HPLC with fluorescence detection [13] rather than the GC procedure requiring propofol derivatization [11] . As seen in Figure 1, the main difference between the investigated systems is the level of unbound propofol percentage.…”

“…The decrease of total propofol concentration below 3 µg/mL leads to an increase of its free fraction [11] . At very low total propofol concentrations, its free fraction tends to reach 100% [11] .…”

“…At very low total propofol concentrations, its free fraction tends to reach 100% [11] . The cited results were obtained when investigat-ing the total and free drug concentration in human plasma and human serum albumin (HSA) solutions spiked with an ethanolic solution of propofol.…”

Formation of the N-methylpyridinium ether derivative of propofol to improve sensitivity, specificity and reproducibility of its detection in blood by liquid chromatography–mass spectrometry

“…It is assumed that pharmacological effects are restricted to the availability of the unbound form. At low plasma concentrations, the proportion of free propofol tends to increase considerably [4].…”