Influence of Rangelia vitalii (Apicomplexa: Piroplasmorida) on Copper, Iron, and Zinc Bloodstream Levels in Experimentally Infected Dogs

Silva, Aleksandro S. Da; França, Raqueli Teresinha; Costa, Márcio Machado; Paim, Carlos Breno; Paim, Francine C.; Santos, Carlos Honorato Schuch; Flores, Érico M.M.; Eilers, Tiago L.; Mazzanti, Cinthia M.; Monteiro, Sílvia González; Amaral, Carlos Henrique do; Lopes, Sonia T.A.

doi:10.1645/ge-2985.1

Cited by 4 publications

(5 citation statements)

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“…Serum copper concentrations are also higher in human patients suffering from hepatic disorders [ 31 ], and elevated Cu:Zn ratios have been proposed as markers of disease in patients with hepatic cirrhosis [ 31 ] or hepatocellular carcinoma [ 32 ]. The role of Cu in the acute-phase protein ceruloplasmin in controlling acute inflammatory-infectious disorders, leading to a marked increase in Cu serum concentrations, is well known in both dogs [ 22 , 33 ] and humans [ 34 ]. There is evidence of increased concentrations of Cu and decreased concentrations of Fe and Zn in serum of dogs infected with Hepatozoon canis [ 22 ] and with Rangelia vitalii (Apicomplexa: Piroplasmorida) [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…The role of Cu in the acute-phase protein ceruloplasmin in controlling acute inflammatory-infectious disorders, leading to a marked increase in Cu serum concentrations, is well known in both dogs [ 22 , 33 ] and humans [ 34 ]. There is evidence of increased concentrations of Cu and decreased concentrations of Fe and Zn in serum of dogs infected with Hepatozoon canis [ 22 ] and with Rangelia vitalii (Apicomplexa: Piroplasmorida) [ 33 ]. The concentrations of Cu in human serum are increased in various carcinomas [ 32 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

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Serum Concentrations of Essential Trace and Toxic Elements in Healthy and Disease-Affected Dogs

Cedeño

Miranda

Galdo

et al. 2020

Animals

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This study was designed (i) to establish reference ranges for the essential trace element and background levels of toxic element exposure in the healthy/normal dog population, and (ii) to evaluate whether trace element concentrations vary in dogs suffering from different pathologies. Blood serum samples were collected from 187 healthy and diseased dogs at the Veterinary Teaching Hospital, Faculty of Veterinary Medicine, University of Santiago de Compostela (northwest Spain). The samples were acid digested, and the concentrations of trace elements (Co, Cr, Cu, Fe, Mn, Mo, Ni, Se and Zn) and toxic elements (As, Cd, Hg and Pb) were determined by inductively coupled plasma-mass spectrometry (ICP-MS). This enabled us to establish reference ranges for the essential trace elements and the level of toxic element exposure in dogs, and to identify several clinical situations associated with variations in trace elements in serum. Relative to concentrations in healthy control dogs, statistically significant differences were observed in the concentrations of Cu (significantly higher in hepatic, inflammatory/infectious and oncological categories), Mo (significantly higher in renal category), Se (significantly lower in gastrointestinal category) and Zn (significantly lower in gastrointestinal, inflammatory/infectious and renal categories). Trace element concentrations can be a cause or consequence of disease, and the study findings indicate that trace element determination in serum provides useful information on the pathogenesis of certain diseases. Further research on the serum concentrations of trace elements, particularly in relation to other biochemical parameters and diagnostic tools, may provide valuable information for the diagnosis of diseases in dogs and the disease prognosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Serum Concentrations of Essential Trace and Toxic Elements in Healthy and Disease-Affected Dogs

Cedeño

Miranda

Galdo

et al. 2020

Animals

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“…The infected dogs showed no bleeding (a common finding in rangeliosis) despite severe thrombocytopenia, reduction of platelet activity, and alteration in the concentrations of nucleotides and nucleosides present in platelets, participants in the process of homeostasis [18,19]. The infection by R. vitalii caused in these dogs reduction in serum levels of iron, zinc, and copper [20], as well as oxidative stress with increased lipid peroxidation, protein oxidation and, consequently, increase of antioxidant status to reduce cellular injury [21]. …”

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confidence: 99%

Increased Cytokine and Nitric Oxide Levels in Serum of Dogs Experimentally Infected with Rangelia vitalii

et al. 2013

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This study aimed to measure the levels of interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), interleukin 1 (IL-1), interleukin 6 (IL-6), and nitrite/nitrate (NOx) in serum of dogs experimentally infected with Rangelia vitalii. Twelve female mongrel dogs were divided into 2 groups; group A (uninfected controls) composed by healthy dogs (n=5) and group B consisting of dogs inoculated with R. vitalii (n=7). Animals were monitored by blood smear examinations, which showed intraerythrocytic forms of the parasite on day 5 post-infection (PI). Blood samples were collected through the jugular vein on days 0, 10, and 20 PI to determine the serum levels of IFN-γ, TNF-α, IL-1, IL-6, and NOx. Cytokines were assessed by ELISA quantitative sandwich technique, and NOx was measured by the modified Griess method. Cytokine levels (IFN-γ, TNF-α, IL-1, and IL-6) were increased (P<0.01) in serum of infected animals. Serum levels of NOx were also increased on days 10 PI (P<0.01) and 20 PI (P<0.05) in infected animals. Therefore, the infection with R. vitalii causes an increase in proinflammatory cytokines and nitric oxide content. These alterations may be associated with host immune protection against the parasite.

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“…It is also involved in Cu transport and antioxidant defense [30], the latter by inhibiting Cu ion-stimulated formation of reactive oxidants and the scavengers H 2 O 2 and superoxide. Canine serum ceruloplasmin levels are increased during infection, inflammation, and trauma [17,18,31]. The increase is greater and becomes evident earlier than in humans, peaking at about two times normal values on the fourth day after surgery [31].…”

Section: Discussionmentioning

confidence: 99%

“…Although information about the effects of trace element deficiencies is very scarce in veterinary medicine, there is evidence for an association between mineral imbalances and some pathological disorders. For example, Se and Zn deficiencies have been described in dogs suffering from diarrhea, irrespective of the etiology [15]; low levels of Se have been associated with a higher incidence of neoplasms and allergies [16]; increased serum concentrations of copper (Cu) and decreased serum concentrations of iron (Fe) and Zn have been found in dogs infected with Hepatozoon canis [17] and Rangelia vitalii (Apicomplexa: Piroplasmorida) [18]; and increased whole blood manganese (Mn) concentrations have been reported in dogs with primary hepatitis [19] and epilepsy [20].…”

Section: Introductionmentioning

confidence: 99%

Trace Element Levels in Serum Are Potentially Valuable Diagnostic Markers in Dogs

Cedeño

Miranda

Galdo

et al. 2020

Animals

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The objective of this study was to obtain information about the role of trace element imbalance in the pathogenesis of certain diseases in dogs and to evaluate the suitability of trace element profiling as an additional tool in the diagnosis. Serum trace element concentrations (copper, molybdenum, selenium and zinc) were measured in a cohort of healthy (control) dogs (n = 42) and dogs affected by hepatic (n = 25), gastrointestinal (n = 24), inflammatory/infection (n = 24), and renal (n = 22) diseases. These data were analyzed together with data on basic biochemical parameters (alanine aminotransferase, alkaline phosphatase, blood urea nitrogen, creatinine, albumin, globulin, and glucose) by using chemometric techniques. The chemometric analysis revealed distinctive association patterns between trace elements and biochemical parameters for each clinical disorders. The findings provide clear evidence for the important role of trace elements in disease, particularly in relation to acute phase reactions, with serum copper providing an indirect measurement of ceruloplasmin (positive acute-phase protein) and serum selenium and zinc acting as negative acute phase reactants. Molybdenum may also be a suitable marker of incipient renal disease. Thus, the analysis of trace element profiles, by multielement techniques, in a single serum sample would be a valuable additional tool for the diagnosis of certain diseases.

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Influence of Rangelia vitalii (Apicomplexa: Piroplasmorida) on Copper, Iron, and Zinc Bloodstream Levels in Experimentally Infected Dogs

Cited by 4 publications

References 19 publications

Serum Concentrations of Essential Trace and Toxic Elements in Healthy and Disease-Affected Dogs

Serum Concentrations of Essential Trace and Toxic Elements in Healthy and Disease-Affected Dogs

Increased Cytokine and Nitric Oxide Levels in Serum of Dogs Experimentally Infected with Rangelia vitalii

Trace Element Levels in Serum Are Potentially Valuable Diagnostic Markers in Dogs

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