Influence of Recovery Method and Centrifugation on Epididymal Sperm from Collared Peccaries (<i>Pecari tajacu</i>Linnaeus, 1758)

Bezerra, José Artur Brilhante; Silva, Alexandre Rodrigues; Peixoto, Gislayne Christianne Xavier; Silva, Mariana de Araújo da; Oliveira, Moacir Franco de

doi:10.2108/zs130149

Cited by 8 publications

(10 citation statements)

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“…We observed that both flotation and retrograde flushing methods proved to be effective for sperm recovery in this species, similarly as reported for collared peccaries ( Pecari tajacu ; Bezerra et al . ). On the other hand, we highlight the ease and convenience at conducting the flotation for epididymal sperm recovery in this rodent, given the small size of its epididymis.…”

Section: Discussionmentioning

confidence: 97%

Characterization of epididymal sperm from Spix's yellow‐toothed cavies (Galea spixii Wagler, 1831) recovered by different methods

et al. 2016

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da Silva, A.M., Bezerra, J.A.B., Campos, L.B., Praxedes, E.C.G., Lima, G.L. and Silva, A.R. 2017. Characterization of epididymal sperm from Spix's yellow-toothed cavies (Galea spixii Wagler, 1831) recovered by different methods. -Acta Zoologica (Stockholm) 98: 285-291.The aim of the present study was to characterize the epididymal sperm of Spix's yellow-toothed cavy (Galea spixii) through two different recovery methods. Nine sexually mature males were euthanized and the complexes, testes-epididymis, were dissected. For each animal, one epididymis was processed by flotation method and the other was processed by retrograde flushing method, both using a TES-based buffered media. After recovery, we evaluated the sperm for motility, vigour, viability, functional membrane integrity and morphology. Morphometric data from the different sperm regions were evaluated by using an appropriate software. After recovery, both methods provide similar values for all the sperm parameters, aiming the recovery of more than 300 9 10 6 sperm, presenting >50% motile sperm, with normal morphology and functional membrane. The total sperm length in this sperm was 48.87 AE 0.1, and the sperm head presented 9.4%, on average. A notable characteristic was the prominent acrosome found in the G. spixii sperm. In conclusion, we demonstrate that either flotation or retrograde flushing methods are suitable for the recovery of sperm from cauda epididymis of Spix's yellow-toothed cavies.

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Section: Discussionmentioning

confidence: 97%

Characterization of epididymal sperm from Spix's yellow‐toothed cavies (Galea spixii Wagler, 1831) recovered by different methods

et al. 2016

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“…After slaughtering, the testis–epididymis complexes (CTEs) were removed, covered with gauze humidified with sterile salt solution (NaCl 0.9%), and immediately transported to the laboratory at room temperature. The CTEs were dissected and cauda epididymis, including 4 to 5 cm of the proximal vas deferens, were isolated and washed with salt solution to remove blood and cell debris (Bezerra et al ., 2014).…”

Section: Methodsmentioning

confidence: 99%

“…Currently, studies on peccaries’ germplasm conservation have been mainly focused on the use of ejaculated sperm (Castelo et al ., 2010a; Silva et al ., 2012; Souza et al ., 2016; Campos et al ., 2017) and there has been only one study concerning the recovery and characterization of their epididymal sperm (Bezerra et al ., 2014). The use of epididymal sperm represents an important tool for their conservation because it allows the preservation of genetic material from valuable wild and captive individuals, even in the event of an unexpected death (Vilela et al ., 2017).…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of collared peccary (Pecari tajacu L., 1758) epididymal sperm using extenders based on Tris and powdered coconut water (ACP®-116c)

Bezerra

Silva

Sousa

et al. 2018

Zygote

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SummaryThe aim of this study was to establish a functional freezing-thawing protocol for epididymal sperm of collared peccaries (Pecari tajacu L., 1758) by comparing different extenders. The epididymal sperm from 12 sexually mature males was recovered by retrograde flushing using Tris-based or coconut water-based (ACP®-116c) extenders. After initial evaluation, samples were diluted and frozen with the same extenders to which 20% egg yolk and 6% glycerol were added. After 2 weeks, thawing was performed at 37°C/60 s and sperm motility, vigour, morphology, functional membrane integrity, sperm viability, sperm plasma membrane integrity, and a computer-assisted semen analysis (CASA) were assessed. In addition, to evaluate the survival of frozen-thawed sperm, a thermal resistance test (TRT) was executed. Samples preserved using Tris were in better condition compared with those preserved using ACP®, showing higher values for most assessments performed, including CASA and the TRT (P<0.05). After determining Tris to be the better of the two extenders, additional samples were thawed using different thawing rates (37°C/60 s, 55°C/7 s, 70°C/8 s). Sperm thawed at 37°C/60 s had the greatest preservation (P<0.05) of viability (54.1 ± 5.9%) and functional membrane integrity (43.2 ± 5.4%), and had higher values for various CASA parameters. In conclusion, we suggest the use of a Tris-based extender added to egg yolk and glycerol for the cryopreservation of epididymal sperm obtained from collared peccaries. In order to achieve better post-thawing sperm quality, we suggest that samples should be thawed at 37°C/60 s.

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“…On the other hand, the retrograde washing is accomplished by injecting a buffered solution at the vas deferens, as it was described for the agouti [21]. In some species, such as collared peccaries [22] and yellow-toothed cavies (Galea spixii) [23], both recovery methods can be conducted with no notorious differences on sperm quality.…”

Section: Epididymal Sperm Retrievalmentioning

confidence: 99%

Assisted Reproductive Techniques on South American Wild Mammals

Silva¹,

Pereira²,

Lima³

et al. 2016

Insights From Animal Reproduction

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For South American wild mammals, assisted reproductive techniques (ARTs) have been established in accordance with the procedures used for domestic mammals. However, often, it is not possible to infer the experimental conditions from one species to another. In general, the use of these ARTs aims the conservation of the animal genetic material, using gamete manipulation, embryo technology by in vivo or in vitro methods, and cloning. In all animals, the previous knowledge of reproductive physiology becomes the starting point for the use of ARTs in the expansion of all species of interest. In this sense, the purpose of this chapter is to develop an approach of theoretical, technical, and applied aspects of the ARTs in South American wild mammals, with emphasis on the state-of-theart and its progress and perspectives.

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Influence of Recovery Method and Centrifugation on Epididymal Sperm from Collared Peccaries (Pecari tajacuLinnaeus, 1758)

Cited by 8 publications

References 30 publications

Characterization of epididymal sperm from Spix's yellow‐toothed cavies (Galea spixii Wagler, 1831) recovered by different methods

Characterization of epididymal sperm from Spix's yellow‐toothed cavies (Galea spixii Wagler, 1831) recovered by different methods

Cryopreservation of collared peccary (Pecari tajacu L., 1758) epididymal sperm using extenders based on Tris and powdered coconut water (ACP®-116c)

Assisted Reproductive Techniques on South American Wild Mammals

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