Influence of reproductive status on in vitro oocyte maturation in dogs

Rodrigues, Berenice de Ávila; Rodrigues, José Luiz

doi:10.1016/s0093-691x(02)01301-8

Cited by 38 publications

(7 citation statements)

References 14 publications

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“…The stained oocytes were mounted on a glass slide with a 100% of glycerol drops. The chromatin state and position of nucleus were assessed under a stereomicroscope with UV light to determine the meiotic stages as previously described [30], and oocytes with an extruded first polar body representing the MII stage were identified as shown in Fig. 4.…”

Section: Evaluation Of Oocyte Maturationmentioning

confidence: 99%

Oocyte maturation-related gene expression in the canine oviduct, cumulus cells, and oocytes and effect of co-culture with oviduct cells on in vitro maturation of oocytes

Lee

Kim

et al. 2017

J Assist Reprod Genet

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Section: Evaluation Of Oocyte Maturationmentioning

confidence: 99%

Oocyte maturation-related gene expression in the canine oviduct, cumulus cells, and oocytes and effect of co-culture with oviduct cells on in vitro maturation of oocytes

Lee

Kim

et al. 2017

J Assist Reprod Genet

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“…1998, 2006; Hewitt and England 1999; Bogliolo et al. 2002; de Avila Rodrigues and Rodrigues 2003; Luvoni et al. 2003; Rodrigues and Rodrigues 2003; Cui et al.…”

Section: Introductionmentioning

confidence: 99%

Effect of Follicle Stimulation Hormone and Luteinizing Hormone on Cumulus Cell Expansion and In Vitro Nuclear Maturation of Canine Oocytes

Hs¹,

Y²,

Yin³

et al. 2007

Reprod Domestic Animals

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In general, follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play important roles in the regulation of cumulus cell expansion and oocyte maturation. We investigated the effects of supplementation of FSH or LH in in vitro maturation (IVM) medium on the incidence of cumulus cell expansion and nuclear maturation in canine oocytes. Cumulus-oocyte complexes (COCs) were cultured in TCM-199 supplemented with 10% foetal bovine serum (FBS), 1 mg/ml cysteine, 0.2 mm pyruvic acid and different concentrations of FSH or LH (control, 0.5, 5 or 50 microg/ml) at 38.5 degrees C, 5% CO(2) in air for 72 h. The cumulus cell expansion was measured by microscopic visualization, and nuclear maturation of denuded oocytes was determined by staining with 10 microg/ml Hoechst33342 for 30 min. The cumulus cell expansion in the 5 microg/ml FSH group (397.2 +/- 64.3 microm) was significantly higher than those in the control, 0.5, and 50 microg/ml FSH groups (168.3 +/- 19.1, 286.0 +/- 69.7 and 300.0 +/- 84.3 microm, respectively; p < 0.05). However, there was no difference in cumulus cell expansion among the control, 0.5, 5 and 50 microg/ml LH groups (165.6 +/- 20.2, 160 +/- 26.5, 172 +/- 20.5 and 168 +/- 23.1 microm, respectively; p > 0.05). After 72 h of IVM, the proportion of nuclear development to the MI-MII stage in the 0.5 microg/ml FSH group (15.1%) was higher than those in the control, 0.5 and 50 microg/ml FSH groups (0.9%, 6.5% and 8.0%, respectively; p < 0.05). However, there was no significant difference in nuclear maturation to the MI-MII stage among control, 0.5, 5 and 50 microg/ml LH groups (4.6%, 2.3%, 5.4% and 8.6%, respectively; p > 0.05). This study indicated that a FSH supplement in IVM medium can increase cumulus cell expansion and nuclear maturation, while the nuclear maturation rate remained low. Further studies are required to improve the nuclear development to the MI-MII stages in canine oocytes.

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“…Van Soom et al (2007) observed that COCs cultured for 48 h showed enhanced rates of CC apoptosis leading to chromatin degeneration or arrest. It has been suggested that this may be an explanation for the high degeneration levels observed in canine COCs matured in vitro (de Avila Rodrigues & Rodrigues, 2003). It was suggested that apoptosis in CCs may be one of the factors responsible for the inhibition of oocyte meiotic competence.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, adenosine triphosphate (ATP) depletion, which is associated with reductions in energy levels in COCs cultured in vitro , may serve as a possible reason for COCs degeneration, as ATP is responsible for maintenance of homeostasis and cell survival (St John, 2002). It has been suggested that this may be an explanation for the high degeneration levels observed in canine COCs matured in vitro (de Avila Rodrigues & Rodrigues, 2003). Furthermore it was also shown that spontaneous apoptosis may occur in COCs during suboptimal culture and specific components in medium influenced positively or negatively on the cell death or survival (Yuan et al , 2005).…”

Section: Discussionmentioning

confidence: 99%

“…progesterone and estradiol, on the meiotic competence of canine oocytes was determined. However, decreased numbers of canine oocytes have been found to reach the MII stage, as compared with other species such as bovine, (Luvoni et al, 2001; de Avila Rodrigues & Rodrigues, 2003;Rodrigues Bde et al, 2004;Songsasen & Wildt, 2007), and only 20-25% of canine oocytes have been shown to be meiotically competent after IVM (Willingham-Rocky et al, 2003;Songsasen & Wildt, 2007;Rodrigues & Rodrigues, 2010;Kempisty et al, 2012). Until now, only one morula and one blastocyst have been produced after IVF in dogs (Otoi et al, 2000(Otoi et al, , 2004.…”

Section: Introductionmentioning

confidence: 99%

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Expression and cellular distribution of zona pellucida glycoproteins in canine oocytes before and afterin vitromaturation

Kempisty

Piotrowska

Bukowska

et al. 2014

Zygote

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This study was aimed at investigating zona pellucida glycoproteins (ZP) ZP2, ZP3 mRNA expression as well as ZP3, ZP4 (ZPB) protein distribution before and after in vitro maturation (IVM) in canine oocytes. The cumulus-oocyte complexes (COCs) were recovered from 27 anoestrous mongrel bitches and matured for 72 h in TCM199 medium. The canine COCs were analysed before and after IVM. Using real-time quantitative polymerase chain reaction (RQ-PCR), both groups of oocytes were analysed for detection of ZP2 and ZP3 mRNA profiles as well as using confocal microscopic analysis for observation of ZP3 and ZP4 protein distribution. In post-IVM canine oocytes an increase in transcript content of ZP2 and ZP3 genes as well as a decrease in ZP3 and ZP4 protein levels were observed when compared with pre-IVM oocytes. Moreover, the ZP4 protein before IVM was significantly distributed in the peripheral area of cytoplasm, whereas after IVM it was localized rather than in the entire cytoplasm. In contrast, the ZP3 protein was found both before and after IVM was distributed in the peripheral area of the cytoplasm. In conclusion, we suggest that the expression of ZP2 and ZP3 genes is associated with the maturation stage of canine oocytes, as higher mRNAs levels were found after IVM. However, a decreased expression of ZP3 and ZP4 proteins after IVM suggests maturation-dependent down-regulation of these protein translations, which may result in disturbed fertilization.

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Influence of reproductive status on in vitro oocyte maturation in dogs

Cited by 38 publications

References 14 publications

Oocyte maturation-related gene expression in the canine oviduct, cumulus cells, and oocytes and effect of co-culture with oviduct cells on in vitro maturation of oocytes

Oocyte maturation-related gene expression in the canine oviduct, cumulus cells, and oocytes and effect of co-culture with oviduct cells on in vitro maturation of oocytes

Effect of Follicle Stimulation Hormone and Luteinizing Hormone on Cumulus Cell Expansion and In Vitro Nuclear Maturation of Canine Oocytes

Expression and cellular distribution of zona pellucida glycoproteins in canine oocytes before and afterin vitromaturation

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