Influence of salt and acetonitrile on the capillary zone electrophoresis analysis of imatinib in plasma samples

Abstract

Influence of salt and acetonitrile on the capillary zone electrophoresis analysis of imatinib in plasma samplesA simple, sensitive, specific, and cost-effective analytical methodology was developed for the analysis of human plasma samples spiked with imatinib by CZE with on-line UV detection in the context of Therapeutic Drug Monitoring. Several analytical conditions such as the ionic strength (I) and the pH of the BGE composed of citric acid and -amino caproic acid were studied in regards of the presence of sodium chloride (NaCl) in plasma samples (1% m/v). Computer simulations (Simul software) were used to confirm the experimental results and to understand imatinib electrophoretic behavior in the presence of NaCl. Furthermore, the advantages of adding ACN to the sample containing NaCl to combine efficient protein precipitation and on-line CZE stacking of imatinib were demonstrated. LOD and LOQ values of 48 and 191 ng/mL were obtained from plasma sample supernatant after protein precipitation with ACN, which is much lower than mean imatinib plasma level observed for patients treated by imatinib mesylate (about 1000 ng/mL). Good linearity was obtained in the concentration range 191-5000 ng/mL (R 2 > 0.997). RSD of less than 1.68% and 2.60% (n = 6) for migration times and corrected peak areas, respectively, were observed at the LOQ.

Dynamic computer simulations of electrophoresis: 2010–2020

“…Furthermore, computer simulations with SIMUL5 were employed to characterize the field‐enhanced sample injection process and associated formation of moving boundaries in a process that was coupled to sweeping [ 141 ]. In the context of the CZE analysis of imatinib in plasma samples, computer simulations with SIMUL5 were used to confirm the experimental results and to understand the electrophoretic behavior of imatinib in the presence of NaCl [ 142 ].…”

Facile and Sensitive Acetylene Black-Based Electrochemical Sensor for the Detection of Imatinib

“…Terefore, IMA monitoring is necessary for clinical treatment [4]. Tus far, several analytical techniques have been applied to the detection of IMA, such as electrophoresis [5], high-performance liquid chromatography [6], and UV-vis spectroscopy [7]. However, due to the expensive equipment, long analysis process, and complicated operation steps of the existing techniques, an economical, fast, simple, and efcient method for IMA determination is urgently needed.…”

Detection of Imatinib Based on Electrochemical Sensor Constructed Using Biosynthesized Graphene-Silver Nanocomposite

“…Currently, the detection methods for imatinib include UV-vis spectroscopy (Grante et al, 2014), HPLC (Roth et al, 2010), and electrophoresis (Li et al, 2012;Ahmed et al, 2019). Although these methods can be used for the rapid detection of imatinib, they all have their own drawbacks.…”