Influence of season and pituitary pars intermedia dysfunction on hair cortisol concentration in horses

Banse, Heidi E.; Getachew, Fitsum; Levy, Morris; Smits, Judit E. G.

doi:10.1016/j.domaniend.2019.07.003

Cited by 10 publications

(9 citation statements)

References 26 publications

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“…The range of provided HCC was from 1.3 pg mg −1 (this work) to 510 pg mg −1 [17]. In a few, other cases, the HCCs were only reported in the graphs or figures [13,16]. It is noteworthy that the detection/quantitation of horsehair cortisol has been carried out almost exclusively with immunoassay techniques (i.e., EIA and RIA) except for the previous published paper [4].…”

Section: Overview Of the Methods For Hcc Determinationmentioning

confidence: 78%

“…In the same year, an illness condition such as the equine squamous gastric disease (ESGD) was evaluated in terms of stress by Prinsloo and coworkers [12] using an ELISA method. To the best of our knowledge, in the following years, only other seven manuscripts have been published [1,[13][14][15][16][17][18]. With regard to sampling body region, mane and withers have been chosen more often [10,12,15,19].…”

Section: Overview Of the Methods For Hcc Determinationmentioning

confidence: 99%

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Determination of hair cortisol in horses: comparison of immunoassay vs LC-HRMS/MS

et al. 2022

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The measure of hair cortisol concentration (HCC) is becoming an emerging approach to monitor mid-/long-term stress in animals, so it is more and more important to develop accurate and reliable methods. In the light of this, the aim of the present study was to compare mane HCCs of 47 horses with different managements, by means of an immunoassay (ELISA) and liquid chromatography coupled to hybrid high-resolution mass spectrometry (LC-HRMS/MS). After the washing step, the ground hair was extracted with methanol. The extract was evaporated and redissolved in two different aqueous solutions, depending on the detection technique. The methods were validated according to EMA guideline for bioanalytical method validation, in the range 2–50 pg mg−1 (ELISA) and 1–100 pg mg−1 (LC-HRMS/MS). Satisfactory quantitative performances were obtained for both of the approaches, but this latter demonstrated better precision. The detected concentrations in real samples were encompassing the range 1.3–8.8 pg mg−1 and 2.0–17.9 pg mg−1 by means of LC-HRMS/MS and ELISA, respectively. Overall, HCCs measured with ELISA technique were 1.6 times higher. The overestimation of immunoassay results might be caused by cross-reactivity phenomena of laboratory reagents and other structurally similar hormones present in the mane. Graphical abstract

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Section: Overview Of the Methods For Hcc Determinationmentioning

confidence: 78%

Section: Overview Of the Methods For Hcc Determinationmentioning

confidence: 99%

Determination of hair cortisol in horses: comparison of immunoassay vs LC-HRMS/MS

et al. 2022

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“…Several studies in domestic and wild mammals have revealed different sources of variation in hair steroids levels [ 13 ], depending on hair specific characteristics (e.g., body region [ 17 ], hair growth phase [ 18 ], or colour [ 19 , 20 ]), intrinsic animal-based characteristics (e.g., age [ 19 , 21 ], sex [ 22 , 23 ] or reproductive status [ 24 , 25 ]) and, finally, external factors (e.g., season [ 26 , 27 ]). All of the previously presented components should be considered when using hair to assess hormonal levels as stress indicators.…”

Section: Introductionmentioning

confidence: 99%

Hair Cortisol, Testosterone, Dehydroepiandrosterone Sulfate and Their Ratios in Stallions as a Retrospective Measure of Hypothalamic–Pituitary–Adrenal and Hypothalamic–Pituitary–Gonadal Axes Activity: Exploring the Influence of Seasonality

Olvera-Maneu

Carbajal

Gardela

et al. 2021

Animals

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The monitoring of stress physiology includes studying a wide range of endocrinological mechanisms, which can be assessed using multiple tissue samples. This study aimed to evaluate the seasonal variations of hair C, T and DHEA-S in horses for a whole year, as well as to assess the variations between seasons of C/DHEA-S and T/C ratios as a retrospective measure of the hypothalamic–pituitary–adrenal and hypothalamic–pituitary–gonadal axis activity. Ten pure-breed Menorca stallions were included in the study. The hair samples were collected approximately every two months following the shave-reshave method caudally to the sternum. After a methanol-based extraction, samples were analyzed by enzyme immunoassay for cortisol, testosterone, and dehydroepiandrosterone sulphate. Following our findings, we detected that cortisol, testosterone and dehydroepiandrosterone sulphate were significantly affected by seasonality, with the highest values of cortisol during summer and the lowest values of testosterone during spring. Dehydroepiandrosterone sulphate concentrations were increased in autumn compared to the other studied periods. Additionally, the studied hormone ratios showed variations between seasons. To conclude, season should, therefore, be considered when assessing sexual and stress hormones in stallion hair, since this variable can be a potential influencing factor and led to misinterpretations.

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“…[7][8][9] Characteristic clinical signs of advanced PPID are hypertrichosis and laminitis. [9][10][11][12][13] In addition, other signs are reported such as weight loss, pendulous abdomen, polydipsia, polyuria, reproductive disorders, and respiratory and urinary tract infections associated with altered immunity. 1,3,9 In the past, the 19-hour overnight dexamethasone suppression test has been recommended for PPID diagnosis.…”

Section: Introductionmentioning

confidence: 99%

“…Signs may be related to functional deterioration stemming from physical compression of the pars distalis, pars nervosa, and hypothalamus which is associated with hyperplasia of the pars intermedia and abnormal secretion of related hormones, including α‐melanocyte stimulating hormone (α‐MSH), adrenocorticotropic hormone (ACTH), dopamine, and cortisol 7–9 . Characteristic clinical signs of advanced PPID are hypertrichosis and laminitis 9–13 . In addition, other signs are reported such as weight loss, pendulous abdomen, polydipsia, polyuria, reproductive disorders, and respiratory and urinary tract infections associated with altered immunity 1,3,9 …”

Section: Introductionmentioning

confidence: 99%

Relationship between endogenous plasma adrenocorticotropic hormone concentration and reproductive performance in Thoroughbred broodmares

Tsuchiya

Noda

Ikeda

et al. 2021

Veterinary Internal Medicne

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Background: Pituitary pars intermedia dysfunction (PPID) is an endocrine disorder the clinical signs of which include infertility, but few reports have examined the relationship between PPID and reproductive performance in broodmares.Hypothesis/Objective: Broodmares with infertility of unknown etiology were screened for PPID using baseline endogenous plasma ACTH concentrations, and its relationship with subsequent reproductive performance was analyzed.Animals: Sixty-seven Thoroughbred broodmares.Methods: Adrenocorticotropic hormone concentrations were measured in autumn.The broodmares were divided into 3 groups based on endogenous plasma ACTH concentration (low, <50 pg/mL; intermediate 50-100 pg/mL; and high >100 pg/mL) as recommended by the Equine Endocrinology Group. We compared these results with those of other blood tests, clinical examinations, subsequent conception, and frequency of normal delivery.Results: The numbers of horses per group (percentage) were 22 (32.8%), 20 (29.9%), and 25 (37.3%). The mean ± SD ages (years) were 11.1 ± 3.4, 12.1 ± 4.5, and 13.0 ± 4.3, with no significant difference among groups. Mean ± SD plasma cortisol concentrations (μg/dL) were 4.0 ± 1.6, 7.1 ± 1.4, and 7.6 ± 1.9, with the intermediate and high ACTH groups having significantly higher results than the low ACTH group (P < .001). Conception rates were 77.3%, 89.5%, and 56.5% and normal delivery rates were 77.2%, 84.2%, and 43.5% with the percentage of each significantly lower in the high ACTH group (P = .02, P = .008, respectively). Conclusions and Clinical Importance:The study group of infertile broodmares may include horses with PPID. The reproductive performance of the high ACTH group was significantly lower than the other groups. Early diagnosis and treatment of infertile broodmares by screening plasma ACTH concentrations could help improve reproductive performance.

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Influence of season and pituitary pars intermedia dysfunction on hair cortisol concentration in horses

Cited by 10 publications

References 26 publications

Determination of hair cortisol in horses: comparison of immunoassay vs LC-HRMS/MS

Determination of hair cortisol in horses: comparison of immunoassay vs LC-HRMS/MS

Hair Cortisol, Testosterone, Dehydroepiandrosterone Sulfate and Their Ratios in Stallions as a Retrospective Measure of Hypothalamic–Pituitary–Adrenal and Hypothalamic–Pituitary–Gonadal Axes Activity: Exploring the Influence of Seasonality

Relationship between endogenous plasma adrenocorticotropic hormone concentration and reproductive performance in Thoroughbred broodmares

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