Influence of Sediment Composition on Apparent Toxicity in a Solid-Phase Test Using Bioluminescent Bacteria

Benton, Michael J.; Malott, Michelle; Knight, Scott S.; Cooper, Charles M.; Benson, William H.

doi:10.1897/1552-8618(1995)14[411:ioscoa]2.0.co;2

Cited by 15 publications

(19 citation statements)

References 1 publication

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“…Cu, Hg, Pb, Zn and ∑PAHs in F1 and with Cd and Pb in F3) and with OM (F1 and F3) and mud (F3) content. The relationship between Microtox® results and OM and mud content has been previously reported (Benton et al 1995;Ringwood et al 1997). It is difficult to establish differences among stations based on this test, as it showed toxicity in the majority of the stations.…”

Section: Discussionmentioning

confidence: 84%

Integrative sediment assessment at Atlantic Spanish harbours by means of chemical and ecotoxicological tools

Montero

Belzunce-Segarra

Menchaca

et al. 2012

Environ Monit Assess

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This study refers to the integrative assessment of sediment quality in three harbour areas at the Spanish Atlantic Coast: Vigo (Northwestern Spain), Bilbao and Pasajes (Northern Spain). At each site, two lines of evidence have been considered: chemical analyses (metal, PAH and PCB concentrations in sediments and ammonia concentration in bioassays) and toxicity tests (Microtox®, Corophium sp. marine amphipod and Paracentrotus lividus sea urchin larvae). Chemical and ecotoxicological results have been integrated by means of a tabular matrix and a multivariate factorial analysis (FA). Highly toxic samples have been characterised in Vigo and Pasajes harbours while Bilbao samples present toxicity levels ranging from non-toxic to moderately toxic. High toxicity is associated with high levels of contaminants whereas confounding factors (ammonia, organic matter and mud) have been identified to be the main cause of low to moderate toxicity. Based on the obtained results, it can be concluded that deriving potential toxicity of sediments based on comparison with Sediment Quality Guidelines (SQGs) is in agreement to toxicity results in areas presenting high levels of contaminants. However, at lower levels of toxicity (low to moderate), the mismatch between the potential toxicity (SQG approach) and the toxicity measured by bioassays is greater, as the former only accounts for chemical concentrations, without considering the interaction between contaminants and the effect of confounding factors. Contrarily, the multivariate analysis seems to be a robust tool for the integration and interpretation of different lines of evidence in areas affected by different sources of contamination.

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Section: Discussionmentioning

confidence: 84%

Integrative sediment assessment at Atlantic Spanish harbours by means of chemical and ecotoxicological tools

Montero

Belzunce-Segarra

Menchaca

et al. 2012

Environ Monit Assess

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“…loss of bacteria adhering to the particles and filtered from the test suspension, such that a significant bias can occur in the interpretation of the results (Benton et al, 1995;Ringwood et al, 1997;Stronkhorst et al, 2003). Ringwood et al (1997) reported that a high rate of false positive results was obtained following exposure to estuarine sediments that were characterised by high silt-clay content.…”

Section: Discussionmentioning

confidence: 99%

A Test Battery Approach for the Ecotoxicological Evaluation of Estuarine Sediments

Davoren¹,

Shúilleabháin²,

Halloran

et al. 2005

Ecotoxicology

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The purpose of this study was to evaluate the overall sensitivity and applicability of a number of bioassays representing multiple trophic levels, for the preliminary ecotoxicological screening (Tier I) of estuarine sediments. Chemical analyses were conducted on sediments from all sampling sites to assist in interpreting results. As sediment is an inherently complex, heterogeneous geological matrix, the toxicity associated with different exposure routes (solid, porewater and elutriate phases) was also assessed. A stimulatory response was detected following exposure of some sediment phases to both the Microtox and algal bioassays. Of the bioassays and endpoints employed in this study, the algal test was the most responsive to both elutriates and porewaters. Salinity controls, which corresponded to the salinity of the neat porewater samples, were found to have significant effects on the growth of the algae. To our knowledge, this is the first report of the inclusion of a salinity control in algal toxicity tests, the results of which emphasise the importance of incorporating appropriate controls in experimental design. While differential responses were observed, the site characterised as the most polluted on the basis of chemical analysis was consistently ranked the most toxic with all test species and all test phases. In terms of identifying appropriate Tier I screening tests for sediments, this study demonstrated both the Microtox and algal bioassays to be more sensitive than the bacterial enzyme assays and the invertebrate lethality assay employing Artemia salina. The findings of this study highlight that salinity effects and geophysical properties need to be taken into account when interpreting the results of the bioassays.

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“…In order to deal with interferences to the SPT, mainly caused by fine particles that cause loss of bacteria (Bulich et al, 1992;Benton et al, 1995;Ringwood et al, 1997), each harbour sample was compared with a reference sediment sample collected from a clean site on the northern Tyrrhenian coast. The reference sample was similar with respect to the particle size and mineralogical composition to the sediments collected in harbours.…”

Section: Microtox ® Assaymentioning

confidence: 99%

The suitability of Corophium orientale (Crustacea, Amphipoda) in harbour sediment toxicity bioassessment

Onorati¹,

Bigongiari²,

Pellegrini³

et al. 1999

Aquatic Ecosystem Health &Amp; Management

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Eco-toxicology represents an important integrative tool of environmental risk assessment. Amphipods is a taxon much recommended for bioassay, but species used are generally from the Atlantic Ocean or the North Sea. None of the Mediterranean species has ever been examined.In an eco-toxicological evaluation of harbour sediment, Corophium orientale, an endemic Mediterranean species, was investigated for suitability to determine sediment quality. Physical-chemical analyses and amphipod bioassays on several contaminated Italian harbour samples were carried out. Two amphipod populations with different sensitivities were used. Microtox ®, a sensitive and reproducible assay procedure, was used in parallel with C. orientale assay in order to ascertain an agreement and/or disagreement between responses. The results of the two bioassay procedures supported the hypothesis that C. orientale, as part of a battery of bioassays, is sensitive to hydro-soluble pollutants contaminating the water column due to dredging activities.

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Influence of Sediment Composition on Apparent Toxicity in a Solid-Phase Test Using Bioluminescent Bacteria

Cited by 15 publications

References 1 publication

Integrative sediment assessment at Atlantic Spanish harbours by means of chemical and ecotoxicological tools

Integrative sediment assessment at Atlantic Spanish harbours by means of chemical and ecotoxicological tools

A Test Battery Approach for the Ecotoxicological Evaluation of Estuarine Sediments

The suitability of Corophium orientale (Crustacea, Amphipoda) in harbour sediment toxicity bioassessment

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