Influence of Selected Factors on Biofilm Formation by Salmonella enterica Strains

Białucha, Agata; Gospodarek‐Komkowska, Eugenia; Kwiecińska-Piróg, Joanna; Skowron, Krzysztof

doi:10.3390/microorganisms9010043

Cited by 2 publications

(2 citation statements)

References 28 publications

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“…It has been described that bacterial mutations affecting flagellar motility have resulted in reduced abilities to create biofilms. Although certain species are recognized to act as co-aggregation factors promoting bacterial adhesion, investigations into food-related biofilms have been restricted to single-species biofilms and brief periods of biofilm formation [ 24 , 25 ]. Here, to assess the role of the flagellum and flagellum-based motility in adhesion and biofilm formation, two types of mutants with distinct flagellar phenotypes were generated for S. Enteritidis and E. coli: fliC mutants, which do not generate flagella, and non-motile motAB mutants.…”

Section: Discussionmentioning

confidence: 99%

The Role of Flagellum and Flagellum-Based Motility on Salmonella Enteritidis and Escherichia coli Biofilm Formation

Vilas Boas,

Castro,

Araújo

et al. 2024

Microorganisms

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Flagellum-mediated motility has been suggested to contribute to virulence by allowing bacteria to colonize and spread to new surfaces. In Salmonella enterica and Escherichia coli species, mutants affected by their flagellar motility have shown a reduced ability to form biofilms. While it is known that some species might act as co-aggregation factors for bacterial adhesion, studies of food-related biofilms have been limited to single-species biofilms and short biofilm formation periods. To assess the contribution of flagella and flagellum-based motility to adhesion and biofilm formation, two Salmonella and E. coli mutants with different flagellar phenotypes were produced: the fliC mutants, which do not produce flagella, and the motAB mutants, which are non-motile. The ability of wild-type and mutant strains to form biofilms was compared, and their relative fitness was determined in two-species biofilms with other foodborne pathogens. Our results showed a defective and significant behavior of E. coli in initial surface colonization (p < 0.05), which delayed single-species biofilm formation. Salmonella mutants were not affected by the ability to form biofilm (p > 0.05). Regarding the effect of motility/flagellum absence on bacterial fitness, none of the mutant strains seems to have their relative fitness affected in the presence of a competing species. Although the absence of motility may eventually delay initial colonization, this study suggests that motility is not essential for biofilm formation and does not have a strong impact on bacteria’s fitness when a competing species is present.

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Section: Discussionmentioning

confidence: 99%

The Role of Flagellum and Flagellum-Based Motility on Salmonella Enteritidis and Escherichia coli Biofilm Formation

Vilas Boas,

Castro,

Araújo

et al. 2024

Microorganisms

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“…Biofilms can grow on a wide variety of surfaces, including those used in the food industry (Iniguez-Moreno et al, 2018). By forming biofilms, microorganisms protect themselves against various environmental inhibitors, including disinfectants (Bialucha et al, 2021;Shatila et al, 2021). It is, thus, difficult to destroy biofilms via standard hygienic procedures (Carrascosa et al, 2021;Dula et al, 2021;Jimenez-Pichardo et al, 2021;Shatila et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of peroxyacetic acid against Salmonella biofilms and as a decontaminant agent in poultry meat

Acar¹,

Cengiz²,

Gulendag³

et al. 2022

J Hellenic Vet Med Soc

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We aimed to investigate the effect of the post-chilling Peroxyacetic Acid (PAA) application on chicken carcasses’ shelf life, PAA treatment on chicken neck skin samples contaminated with S. Enteritidis and S. Typhimurium serotypes, and its activity against single and mixed Salmonella biofilms. Initially, effects of the post-chilling PAA application on chicken carcasses’ shelf life was analyzed. Secondly, the effectiveness of PAA application in chicken neck skin samples which are experimentally contaminated was investigated. Then, biofilm forming capacity of Salmonella enterica serovars were determined, and PAA activity against single and mixed Salmonella biofilms was evaluated. According to the results, in all experimental groups at least 1 log cfu/g reduction was observed on the day of PAA application (0 hour), and we monitored significant decreases of Salmonella counts in all groups after both 100 and 200 ppm PAA treatments within the 6th hour. The combinations of PAA concentrations and duration of application used in the study were insufficient in terms of S. Typhimurium and S. Enteritidis counts in chicken neck skin samples; however, these treatments can be effective on extending the chicken meat’s shelf life. On the other hand, 200 ppm of PAA was effective in reducing S. Typhimurium and S. Enteritidis biofilms. As a mean of 4.8 log/ml reduction were detected against S. Enteritidis and S. Typhimurium biofilms. Moreover, we can conclude from statistical analysis that when biofilm is composed with more than one Salmonella, it is harder to reduce their counts with PAA treatments. However, there is a recommendation of EFSA (European Food Safety Authority) that PAA may be a decontamination agent in poultry production in the future, considering that PAA will not have toxic effects on human health. PAA studies on this subject will be of great importance in the future if it is passed to legislation.

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Influence of Selected Factors on Biofilm Formation by Salmonella enterica Strains

Cited by 2 publications

References 28 publications

The Role of Flagellum and Flagellum-Based Motility on Salmonella Enteritidis and Escherichia coli Biofilm Formation

The Role of Flagellum and Flagellum-Based Motility on Salmonella Enteritidis and Escherichia coli Biofilm Formation

Efficacy of peroxyacetic acid against Salmonella biofilms and as a decontaminant agent in poultry meat

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