Influence of Semen‐collection Frequency on Sperm Quality in Boars, with Special Reference to Biochemical Markers

Strzeżek, J.; Korda, W; Głogowski, J.; Wysocki, Paweł; Borkowski, Kamil

doi:10.1111/j.1439-0531.1995.tb00609.x

Cited by 44 publications

(32 citation statements)

References 31 publications

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“…Further, it was found that high semen collection frequencies affect sperm motility, concentration and total sperm count, and result in increased proportions of abnormal sperm morphology (Strzezek et al 1995). On the molecular level, no significant changes were found in the phospholipid composition of boar sperm between 24 h and 72 h collection frequencies (Johnson et al 1969).…”

mentioning

confidence: 99%

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Frequency of Boar Ejaculate Collection and its Influence on Semen Quality, Pregnancy Rate and Litter Size

Frangež¹,

Gider²,

Kosec³

2005

Acta Vet. Brno

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FrangeÏ R., T. Gider, M. Kosec: Frequency of Boar Ejaculate Collection: its Influence on Semen Quality, Pregnancy Rate and Litter Size. Acta Vet. Brno 2005, 74: 265-273.The aim of this study was to determine the best collection frequency for boar ejaculate, based on semen quality, pregnancy rate and litter size. Over a period of 7 months, 480 ejaculates were obtained, at successive collection frequencies of one, two, three and seven times per week, from 12 mature boars of line 54. For each ejaculate, the volume, progressive motility, spermatozoa concentration, sperm morphology and total number of spermatozoa were determined. Two commercial semen extenders, Beltsville Thawing Solution and Merck, were used for semen dilution and preservation. Smaller ejaculate volumes (P < 0.05), lower sperm concentrations (P < 0.05), and lower total sperm counts per ejaculate (P < 0.05) were obtained at collection frequencies of 7 and 3 than at 2 and 1 times per week. Significantly lower progressive sperm motilities at 7 than at 3, 2 and 1 times per week were observed. 1586 sows were inseminated with semen obtained at the four collection frequencies. The conception in sows was highest when inseminated with semen collected once per week and was significantly (P < 0.05) lower when collected 7 times per week than at the other three collection frequencies. The number of delivered piglets was also highest in sows inseminated with semen collected once per week and significantly(P < 0.05) higher than at collection frequencies of 2 and 3 per week. No significant differences in the semen quality parameters measured or in pregnancy rate were found when using either of the two commercial preservation extenders.These results indicate that the sperm quality decreases with increasing collection frequency and is most pronounced at the collection frequency 7 × per week. Taking into account the fertility rate and litter size, together with the number of semen doses obtained per week, the most suitable ejaculate collection frequency in mature boars of line 54 is two or three times per week. , semen, frequency, sow, artificial insemination, pregnancy rate Boar

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confidence: 99%

“…On the molecular level, no significant changes were found in the phospholipid composition of boar sperm between 24 h and 72 h collection frequencies (Johnson et al 1969). However, some other important biochemical parameters were reported to be altered by high collection frequencies (Strzezek et al 1995(Strzezek et al , 1996.…”

mentioning

confidence: 99%

Frequency of Boar Ejaculate Collection and its Influence on Semen Quality, Pregnancy Rate and Litter Size

Frangež¹,

Gider²,

Kosec³

2005

Acta Vet. Brno

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“…Moreover, it has been shown that in-creased susceptibility of boar spermatozoa to cold-shock treatment was associated with high levels of MDA production, suggesting impairment in sperm function (Strzeżek et al 2000). Research studies have demonstrated that high collection frequency of ejaculates and semen preservation caused membrane damage in the midpiece, resulting in a gradual leakage of AspAT (Strzeżek et al 1995, Gączarzewicz et al 2010.…”

Section: Status Of Structural and Functional Sperm Membranesmentioning

confidence: 99%

“…Acid and alkaline phosphatases are good indicators of the secretory functions of the epididymis and accessory sex glands (Strzeżek et al 1995(Strzeżek et al , 2000. Determination of the activity of these enzymes has been used for the identification of reproductive organ dysfunction.…”

Section: Biochemical Assessment Of the Seminal Plasma Activity Of Selmentioning

confidence: 99%

“…The structural state of sperm chromatin can be assessed, using the radioisotope assay, tritium-labelled actinomycin D ( 3 H-AMD incorporation assay), which has specific affinity for the base pair guanine-cytosine in the DNA structure , Strzeżek et al 1995. According to Strzeżek and Kordan (2003), reducing agents, such as glutathione (reduced), heparin, dithiothreitol, 2-mercaptoethanol and sodium lauryl sulfate, had a very strong decondensing action on boar sperm chromatin structure, as indicated by increased 3 H-AMD labeling.…”

Section: Chromatin and Dna Integritymentioning

confidence: 99%

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Semen quality assessments and their significance in reproductive technology

Kordan¹,

Fraser²,

Wysocki³

et al. 2013

Polish Journal of Veterinary Sciences

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Semen quality assessment methods are very important in predicting the fertilizing ability of persevered spermatozoa and to improve animal reproductive technology. This review discusses some of the current laboratory methods used for semen quality assessments, with references to their relevance in the evaluation of male fertility and semen preservation technologies. Semen quality assessment methods include sperm motility evaluations, analyzed with the computer-assisted semen analysis (CASA) system, and plasma membrane integrity evaluations using fluorescent stains, such as Hoechst 33258 (H33258), SYBR-14, propidium iodide (PI), ethidium homodimer (EthD) and 6-carboxyfluorescein diacetate (CFDA), and biochemical tests, such as the measurement of malondialdehyde (MDA) level. This review addresses the significance of specific fluorochromes and ATP measurements for the evaluation of the sperm mitochondrial status. Laboratory methods used for the evaluation of chromatin status, DNA integrity, and apoptotic changes in spermatozoa have been discussed. Special emphasis has been focused on the application of proteomic techniques, such as two-dimensional (2-D) gel electrophoresis and liquid chromatography mass spectrometry (LC-MS/MS), for the identification of the properties and functions of seminal plasma proteins in order to define their role in the fertilization-related processes.

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