The effects of grain boundaries and surface roughness on tungsten irradiated by 40 keV He ions with a flux of 1.6 × 1017 He+/(m2s) to a fluence of 6.6 × 1021 He/m2 at room temperature were investigated. It was found that rough surfaces can suppress the surface blistering on tungsten. This may be due to the grooves appeared on rough tungsten limiting crack growth, resulting in the release of helium. However, the influence of grain boundaries on denseness of blistering was not obvious except the difference in blistering cap. The number of blister with detached cap on large grain tungsten samples (LGW, with the grain size of ~50 µm) is more than that on small grain tungsten samples (SGW, with the grain size of ~5 µm). In addition, the effect of grain boundaries on helium retention in tungsten cannot be ignored. The total He release from SGW was 2.56 × 1021 He/m2, which was smaller than the value (4.10 × 1021 He/m2) of LGW.