Influence of skeletal site of origin and donor age on 1,25(OH)2D3-induced response of various osteoblastic markers in human osteoblastic cells

Martı́nez, Marı́a Eugenia; Medina, Sonia; Sánchez, Miguel Ángel García; Campo, Ma Teresa del; Esbrit, Pedro; Rodrigo, Ana Moreno; Martínez, Pedro Martínez; Sánchez-Cabezudo, M.; Moreno, Isidro; Garcés, M.V; Munuera, L.

doi:10.1016/s8756-3282(98)00181-1

Cited by 27 publications

(4 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The specificity of the inhibitory effect of alendronate on 1,25(OH) 2 D 3 ‐induced PTHrP decrease in hOB cells was evaluated by measuring secreted osteocalcin in these cells after 1,25(OH) 2 D 3 treatment in the presence or absence of this bisphosphonate. Consistent with previous findings in these cell cultures, (25, 35) 1,25(OH) 2 D 3 was found to stimulate osteocalcin secretion in hOB cells (Fig. 10).…”

Section: Resultsmentioning

confidence: 99%

“…Confluent cells were passaged and again grown to confluence. These (hOB) cells display an osteoblastic phenotype (25, 35) …”

Section: Methodsmentioning

confidence: 99%

“…The sensitivity of this assay is 0.2 pM (36) . Osteocalcin in the cell‐conditioned medium was measured by an immunoradiometric assays (Nichols, San Juan Capistrano, CA, USA), using specific antibodies against human intact osteocalcin (25, 35) . The sensitivity of this assay is 0.05 ng/ml.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Alendronate Interacts With the Inhibitory Effect of 1,25(OH)2D3 on Parathyroid Hormone-Related Protein Expression In Human Osteoblastic Cells

Gómez-García

Esbrit

Carreño

et al. 2003

Journal of Bone and Mineral Research

Self Cite

View full text Add to dashboard Cite

The bisphosphonate alendronate is a potent inhibitor of bone resorption by its direct action on osteoclasts. In addition, there is some data suggesting that alendronate could also inhibit bone resorption indirectly by interacting with osteoblasts. Parathyroid hormone-related protein (PTHrP) produced by osteoblasts and 1,25-dihydroxyvitamin D 3 [1,25(OH) 2 D 3 ] are regulators of bone remodeling, which have interrelated actions in these cells. In this study, we assessed whether alendronate can affect PTHrP expression in the presence or absence of 1,25(OH) 2 D 3 in human primary osteoblastic (hOB) cells from trabecular bone. Cell total RNA was isolated, and semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was carried out using human PTHrPspecific primers. PTHrP in the hOB cell-conditioned medium was analyzed by a specific immunoradiometric assay. We found that PTHrP mRNA and secreted PTHrP were maximally inhibited by 10

show abstract

Section: Resultsmentioning

confidence: 99%

“…Confluent cells were passaged and again grown to confluence. These (hOB) cells display an osteoblastic phenotype (25, 35) …”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Alendronate Interacts With the Inhibitory Effect of 1,25(OH)2D3 on Parathyroid Hormone-Related Protein Expression In Human Osteoblastic Cells

Gómez-García

Esbrit

Carreño

et al. 2003

Journal of Bone and Mineral Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…Like other studies by our group, 45,46 this study determines alkaline phosphatase by measuring its secretion into a conditioned medium. We found that the changes in the secretion of alkaline phosphatase were similar to changes described in intracellular alkaline phosphatase.…”

Section: Discussionmentioning

confidence: 95%

Effects of MA 956 superalloy and ?-alumina particles on some markers of human osteoblastic cells in primary culture

Rodrigo

Martı́nez

Martínez

et al. 2000

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

One of the problems associated with the modern biomaterials used in prostheses is osteolysis, which, although its exact origin is unknown, has been associated with wear particles. Osteoblasts seem to participate directly in this phenomenon. This paper investigates in vitro cellular response to the wear particles from the metal substrate and ceramic covering (alpha-alumina) of a new titanium yttrium aluminum alloy, MA 956, that has been proposed as a biomaterial because of its exceptional mechanical and electrochemical properties. The effect of different sizes (10 and 80 microm) of MA 956 and alpha-alumina particles on osteoblast function was studied in primary human bone cell cultures. Cells were harvested from trabecular bone fragments obtained during knee arthroplasty. Osteoblastic cell response to the particles was measured by assaying C-terminal type I procollagen (PICP), alkaline phosphatase, and osteocalcin secretion, with and without 1.25(OH)(2)D(3) stimulation, in the cell-conditioned medium. Both sizes of MA 956 and alpha-alumina particles decreased PICP secretion in nonstimulated osteoblastic cells, but this secretion was not affected in the cultures stimulated with 1.25(OH)(2)D(3). Only the 10 microm alpha-alumina particles inhibited alkaline phosphatase activity in 1.25(OH)(2)D(3)-stimulated and nonstimulated cultures. The rise in osteocalcin levels after 1.25(OH)(2)D(3) stimulation was lower in the presence of the 10 microm MA 956 particles than in the presence of alpha-alumina particles. Although both materials seem to have directly affected in vitro osteoblastic cell function, the increase in osteocalcin levels after 1.25(OH)(2)D(3) stimulation was lower after exposure to MA 956 particles than the increase observed after exposure to alpha-alumina particles. Therefore, it does not seem that osteocalcin stimulated bone resorption, suggesting that MA 956 would be less likely to provoke osteolysis.

show abstract

Control of Osteoblast Function and Bone Extracellular Matrix Mineralization by Vitamin D

Leeuwen¹,

Driel²,

Pols³

2004

The Skeleton

View full text Add to dashboard Cite

Influence of skeletal site of origin and donor age on 1,25(OH)2D3-induced response of various osteoblastic markers in human osteoblastic cells

Cited by 27 publications

References 38 publications

Alendronate Interacts With the Inhibitory Effect of 1,25(OH)2D3 on Parathyroid Hormone-Related Protein Expression In Human Osteoblastic Cells

Alendronate Interacts With the Inhibitory Effect of 1,25(OH)2D3 on Parathyroid Hormone-Related Protein Expression In Human Osteoblastic Cells

Effects of MA 956 superalloy and ?-alumina particles on some markers of human osteoblastic cells in primary culture

Control of Osteoblast Function and Bone Extracellular Matrix Mineralization by Vitamin D

Contact Info

Product

Resources

About