Influence of sodium salicylate on rosmarinic acid, carnosol and carnosic acid accumulation by Salvia officinalis L. shoots grown in vitro

Kračun-Kolarević, Margareta; Dmitrović, Slavica; Filipović, Biljana; Perić, Marija; Mišić, Danijela; Simonović, Ana; Todorović, Slađana

doi:10.1007/s10529-015-1825-1

Cited by 17 publications

(6 citation statements)

References 29 publications

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“…However, the similar phenomenon was not observed in the A. rugosa cell suspensions in the present study because the experiments were conducted for a relatively short period. There are several biotechnological applications such as callus [1], suspension cell [25], in vitro shoot [33], and hairy root culture [34] used for the production of rosmarinic acid. Among these systems hairy root culture was more predominant in medicinal plants.…”

Section: Discussionmentioning

confidence: 99%

Yeast Extract and Silver Nitrate Induce the Expression of Phenylpropanoid Biosynthetic Genes and Induce the Accumulation of Rosmarinic Acid in Agastache rugosa Cell Culture

et al. 2016

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The present study aimed to investigate the role of yeast extract and silver nitrate on the enhancement of phenylpropanoid pathway genes and accumulation of rosmarinic acid in Agastache rugosa cell cultures. The treatment of cell cultures with yeast extract (500 mg/L) and silver nitrate (30 mg/L) for varying times enhanced the expression of genes in the phenylpropanoid pathway and the production of rosmarinic acid. The results indicated that the expression of RAS and HPPR was proportional to the amount of yeast extract and silver nitrate. The transcript levels of HPPR under yeast extract treatment were 1.84-, 1.97-, and 2.86-fold higher than the control treatments after 3, 6, and 12 h, respectively, whereas PAL expression under silver nitrate treatment was 52.31-fold higher than in the non-treated controls after 24 h of elicitation. The concentration of rosmarinic acid was directly proportional to the concentration of the applied elicitors. Yeast extract supplementation documented the highest amount of rosmarinic acid at 4.98 mg/g, whereas silver nitrate addition resulted in a comparatively lower amount of rosmarinic acid at 0.65 mg/g. In conclusion, addition of yeast extract to the cell cultures enhanced the accumulation of rosmarinic acid, which was evidenced by the expression levels of the phenylpropanoid biosynthetic pathway genes in A. rugosa.

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Section: Discussionmentioning

confidence: 99%

Yeast Extract and Silver Nitrate Induce the Expression of Phenylpropanoid Biosynthetic Genes and Induce the Accumulation of Rosmarinic Acid in Agastache rugosa Cell Culture

et al. 2016

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“…74 Salicylic acid stimulates the biosynthesis of rosmarinic acid in Coleus forskohlii hairy root, 75 while sodium salicylate did not not affect RA production in sage. 50 Elicitation with vanadyl sulfate led to 2.8 times increased RA production compared to the control in Lavandula vera MM cell suspension cultures. 76 Similarly, benzothiadiazole, a synthetic activator of plant systemic acquired resistance, significantly enhanced the elicitation of RA production in suspension cultures of Agastache rugosa.…”

Section: Resultsmentioning

confidence: 95%

“…48 Commonly in vitro tested chemical elicitors are phytohormones, primarily salicylic and jasmonic acid. 49,50 Recently, the application of different synthetic compounds indicated their potential in elicitation of the secondary metabolism in some plants. [51][52][53][54] In this work, synthetic compounds tetraoxane DO63 (1,2,4,5-tetraoxane) and tiophene DOVF15 (2,5-diphenylthiophene) were tested for their elicitation potential of in vitro grown N. cataria and N. pannonica shoots.…”

Section: Introductionmentioning

confidence: 99%

Elicitation effects of synthetic 1,2,4,5-tetraoxane and 2,5-diphenyltiophene in shoot cultures of two Nepeta species

Dmitrović

Škorić

Boljević

et al. 2016

JSCS

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The presented study aimed to investigate the elicitation possibility for the production of main secondary metabolites in Nepeta cataria L. and N. pannonica L. plants, by exposing them to synthetic compounds belonging to tetraoxanes and thiophenes group. The effect of DO63 (1,2,4,5-tetraoxane) and DOVF15 (2,5-diphenylthiophene) on the production of cis-trans-nepetalactone (NL) and rosmarinic acid (RA) in two Nepeta species, was investigated in shoots grown on the culture medium with the addition of synthetic compounds in the concentrations ranging from 0.1 to 2 mg L-1. The content of targeted metabolites in tested in vitro shoots depended on the type and concentration of applied synthetic compounds. Application of DO63 in the concentration range 0.1-1 mg L-1 affected only NL production in both Nepeta species resulting in its increased content, while production of RA was not influenced in the treated shoots. Addition of DOVF15 caused decreased RA content in N. pannonica shoots and an increase in N. cataria shoots, whereas NL production was not affected. The presented results reveal the possibility of DO63 and DOVF15 application for the elicitation of the main secondary metabolites production in species from the genus Nepeta.

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“…It can also alleviate environmental stresses, such as heat (Khan et al 2013), cold (Saleem et al 2020), drought (Sohag et al 2020), UV-B/C radiation (Li et al 2014;Abrun et al 2016), salinity (Elazab and Youssef 2017), and heavy metals toxicity (Sharma et al 2020), and protect plants from a range of pathogens (Bakker et al 2014). The effect of exogenous SA is influenced by factors such as the plant species, its stage of development, tissue and organ type, mode of application, dose and duration of exposure, environmental and culture conditions, and its endogenous level in the plant (Horváth et al 2007;Janda et al 2014;Kračun-Kolarević et al 2015;with 91,258,272 tons (FAOSTAT 2022). This species is an important source of valuable nutrients and bioactive compounds used not only as food but also in cosmetics, therapeutics, and perfumery (Uzodike and Onuoha 2009;Uthpala et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Influence of sodium salicylate on adventitious organogenesis of a commercial cucumber cultivar

Miguel

2023

MSJ

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Bhutia et al 2018). SA application has been reported to modulate plant functions dose-dependently (Maurya et al 2019). Inadequate levels of SA can also lead to oxidative stress (Gondor et al 2016). SA-like effects have been reported with structurally similar compounds, such as NaSA and acetylsalicylic acid (ASA). Some authors have previously described improved in vitro plant regeneration with exogenous SA or ASA. In the present work, it is investigated by applying NaSA.The cucumber (Cucumis sativus L.) is an economically important species belonging to the Cucurbitaceae family, along with melon, watermelon, and squash. Its world production, including gherkins, ranked third among vegetable crops in 2020, Abstract Cucumber is an important vegetable crop belonging to the Cucurbitaceae family. An optimized regeneration system is required to address different biotechnological approaches in this species, such as large-scale multiplication, as well as genome editing and genetic transformation techniques. The aim of this study was to investigate the effect of different concentrations of the phenolic compound sodium salicylate (NaSA), a salicylic acid derivative, on in vitro callus and shoot regeneration of a commercial cultivar of cucumber. Four-day-old cotyledon explants from the slicing cultivar 'Marketer' were employed. Murashige and Skoog (MS)-derived shoot induction medium containing 0.5 mg L-1 indole-3acetic acid (IAA) and 2.5 mg L-1 6-benzylaminopurine (BAP) was supplemented with NaSA. Frequency and shoot number were enhanced by 1.5-fold with NaSA at 0.1 µM. Higher salicylate levels led to increased callus formation and decreased shoot regeneration. The application of sodium salicylate at a specific concentration showed a positive trend in in vitro adventitious organogenesis of a commercial cucumber cultivar. Some probable mechanisms that may underlie the beneficial effects of salicylic acid/salicylates on in vitro regeneration were also discussed.

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Influence of sodium salicylate on rosmarinic acid, carnosol and carnosic acid accumulation by Salvia officinalis L. shoots grown in vitro

Abstract: NaSA can improve C production in sage shoot culture, probably by stimulating the conversion of CA to C.

Cited by 17 publications

References 29 publications

Yeast Extract and Silver Nitrate Induce the Expression of Phenylpropanoid Biosynthetic Genes and Induce the Accumulation of Rosmarinic Acid in Agastache rugosa Cell Culture

Yeast Extract and Silver Nitrate Induce the Expression of Phenylpropanoid Biosynthetic Genes and Induce the Accumulation of Rosmarinic Acid in Agastache rugosa Cell Culture

Elicitation effects of synthetic 1,2,4,5-tetraoxane and 2,5-diphenyltiophene in shoot cultures of two Nepeta species

Influence of sodium salicylate on adventitious organogenesis of a commercial cucumber cultivar

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