Influence of spore and carrier material surface hydrophobicity on decontamination efficacy with condensing hydrogen peroxide vapour

The treatment method to deactivate viable microorganisms from objects or products is termed sterilization. There are multiple forms of sterilization, each intended to be applied for a specific target, which depends on—but not limited to—the thermal, physical, and chemical stability of that target. Herein, an overview on the currently used sterilization processes in the global market is provided. Different sterilization techniques are grouped under a category that describes the method of treatment: radiation (gamma, electron beam, X‐ray, and ultraviolet), thermal (dry and moist heat), and chemical (ethylene oxide, ozone, chlorine dioxide, and hydrogen peroxide). For each sterilization process, the typical process parameters as defined by regulations and the mode of antimicrobial activity are summarized. Finally, the recommended microorganisms that are used as biological indicators to validate sterilization processes in accordance with the rules that are established by various regulatory agencies are summarized.

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“…The findings indicated that surface tension of the condensate can prevent the homogeneous wetting of the surface. [ 132 ]…”

Section: Chemical Sterilizationmentioning

confidence: 99%

Sterilization of Objects, Products, and Packaging Surfaces and Their Characterization in Different Fields of Industry: The Status in 2020

Jildeh

Wagner

Schöning

2021

Physica Status Solidi (a)

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“…Water contact angle measurement of four different CMs was carried out as described above. The results were already published in Eschlbeck et al (2018). The chosen materials and the respective surface hydrophobicity are depicted in Table 1.…”

Section: Carrier Materialsmentioning

confidence: 99%

“…Water contact angle data of the applied CMs and bacterial spores were already published in Eschlbeck et al (2018). For a better understanding of the influence of surface hydrophobicity on the inactivation with gaseous H 2 O 2 , the results are depicted in Figure 1.…”

Section: Surface Hydrophobicity Of Cmsmentioning

confidence: 99%

“…Clearly, CM 2 has the highest roughness whereas CM 1 is smoother and CM 3 exhibits only little roughness. (Eschlbeck et al, 2018). However, atomic force microscopy (AFM) of CM 4 was not possible as the cantilever of the AFM could not identify any roughness of this surface.…”

Section: Surface Roughness Of Cmsmentioning

confidence: 99%

“…Many of the influencing factors, such as temperature, concentration, amount of water in the gas, have been reported elsewhere (Pruß, Stirtzel, & Kulozik, 2012;Unger-Bimczok, Kottke, Hertel, & Rauschnabel, 2008;Wang & Toledo, 1986). The influence of surface hydrophobicity of the carrier material (CM) and the bacterial spores, respectively, during a process with condensate formation has also been studied (Eschlbeck, Seeburger, & Kulozik, 2018). However, little is known about the influence of surface hydrophobicity and surface roughness on the inactivation by vaporized H 2 O 2 without condensate formation.…”

Section: Introductionmentioning

confidence: 99%

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Spore inactivation on solid surfaces by vaporized hydrogen peroxide—Influence of carrier material surface properties

Eschlbeck

Seeburger

Kulozik

2020

Journal of Food Science

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The aim of this study was to investigate the influence of surface hydrophobicity and roughness of carrier materials on the inactivation of bacterial spores with gaseous hydrogen peroxide whereas condensate formation is prevented. Spores of Bacillus subtilis and Bacillus atrophaeus were applied either as single spore culture or as a mixed spore population to simulate natural contamination with microorganisms of different characteristics. Inactivation with gaseous hydrogen peroxide was carried out at 5200 ppm hydrogen peroxide without condensate formation. The inactivation results of B. subtilis and B. atrophaeus spores on carrier materials with varying surface hydrophobicity differed significantly. However, inactivation of the mixed spore populations resulted in similar resistance compared to the single spore batches. The results of this study indicate that surface hydrophobicity most probably has an impact on the inactivation with gaseous hydrogen peroxide whereas surface roughness only plays a minor role.

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Suitability of Sodium Pyruvate as Decomposer of Carried‐over Liquid Hydrogen Peroxide

Belschner¹,

Rosenberger

Atamer

et al. 2023

Chemie Ingenieur Technik

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The aim of the present study was to evaluate the concentration of sodium pyruvate in growth media by using the mostprobable number method to decompose carried-over liquid hydrogen peroxide (H 2 O 2 ) without negative influences on correct enumeration of Geobacillus stearothermophilus spores. An equivalent molar ratio of sodium pyruvate and H 2 O 2 was verified to be sufficient for a complete decomposition. The results showed that by exposition of G. stearothermophilus spores in different liquid hydrogen peroxide and by carry-over of concentrations ranging from 3.56 Á 10 -6 up to 1.69 Á 10 -4 mol, a sodium pyruvate concentration of 0.05 mol L -1 in growth media was most efficient for the recovery of spores.

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Influence of spore and carrier material surface hydrophobicity on decontamination efficacy with condensing hydrogen peroxide vapour

Abstract: The intensity of spore inactivation depends more on other factors than on the wettability of the bacterial spores. However, hydrophobic surfaces lead to faster inactivation effects and should thus be preferred for aseptic packaging technology.

Cited by 5 publications

References 13 publications

Sterilization of Objects, Products, and Packaging Surfaces and Their Characterization in Different Fields of Industry: The Status in 2020

Sterilization of Objects, Products, and Packaging Surfaces and Their Characterization in Different Fields of Industry: The Status in 2020

Spore inactivation on solid surfaces by vaporized hydrogen peroxide—Influence of carrier material surface properties

Suitability of Sodium Pyruvate as Decomposer of Carried‐over Liquid Hydrogen Peroxide

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