End-of-life electric vehicle (EV) batteries can be reused to reduce their environmental impact and economic costs. However, the growth of the second-life market is limited by the lack of information on the characteristics and performance of these batteries. As the volume of end-of-life EVs may exceed the amount of batteries needed for stationary applications, investigating the possibility of repurposing them in mobile applications is also necessary. This article presents an experimental test that can be used to collect the data necessary to fill a battery passport. The proposed procedure can facilitate the decision-making process regarding the suitability of a battery for reuse at the end of its first life. Once the battery passport has been completed, the performance and characteristics of the battery are compared with the requirements of several mobile applications. Mobile charging stations and forklift trucks were identified as relevant applications for the reuse of high-capacity prismatic cells. Finally, a definition of the state of health (SoH) is proposed to track the suitability of the battery during use in the second-life application considering not only the energy but also the power and efficiency of the battery. This SoH shows that even taking into account accelerated ageing data, a repurposed battery can have an extended life of 11 years at 25 °C. It has also been shown that energy fade is the most limiting performance factor for the lifetime and that cell-to-cell variation should be tracked as it has been shown to have a significant impact on the battery life.