Influence of Stearidonic Acid on Lipoprotein Secretion and Fatty Acid Composition in Hepg2 Cells

Abstract

The health benefits of w-3 fatty acids, particularly fish oils, are well known. It is generally recommended to obtain w-3 fatty acids from fatty fish; however, the possible contaminants in fish products may deter this. Thus, supplements of w-3 fatty acids from plant-originated lipids may be of interest. Among these, a-linolenic acid (ALA) and stearidonic acid (SDA) have drawn attention. Thus, the purpose of this research was to test the possible conversion of these fatty acids, particularly SDA, to eicosapentaenoic acid (EPA) using a human hepatoma cell line, HepG2 cells. In addition, even though w-3 fatty acids have been shown to reduce hyperlipidemia, the effects on lipoprotein metabolism are relatively inconsistent. Thus, we also tested w-3 fatty acids along with SDA on apolipoprotein A and B (apo A and B) secretion from this cell line. The results obtained suggest that both ALA and SDA were converted to EPA and they increased apo B secretion, while having no effect on apo A secretion, similar to the effects of EPA and docosahexaenoic acid. Thus, it may be concluded that SDA can be a possible candidate for use as a precursor for EPA and its effect on lipoproteins was similar to that of EPA.3 Corresponding

Caenorhabditis elegansas a Model To Study the Effectiveness and Metabolic Targets of Dietary Supplements Used for Obesity Treatment: The Specific Case of a Conjugated Linoleic Acid Mixture (Tonalin)

“…Although cis-9, trans-11 is the main CLA isomer in natural food, 17 the isomer trans-10, cis-12 is reported to affect energy metabolism and body-fat deposition and composition in mice. 13 Other studies in murine 3T3-L1 adipocytes have shown smaller lipid droplets in differentiated cells treated with trans-10, cis-12 CLA 14 as well as a higher influence on lipid metabolism, energy expenditure and fatty acid oxidation in 3T3-L1 adipocytes. 15,7 In humans results are controversial and studies have not shown either cis-9, trans-11 or trans-10, cis-12 CLA isomer to significantly affect body weight.…”

“…This is consistent with previous studies indicating the trans-10, cis-12 isomer as the active isomer with regard to antiobesity in different experimental models such as cell cultures 7 and animals. 13,16,43 Unfortunately, the antiobesity effects of trans-10, cis-12 CLA isomer in humans remain unclear due to the presence of other intrinsic experimental parameters such as the dose used, study length or exercise. 10,44 Herein, the effectiveness of Tonalin added to a food matrix has been demonstrated.…”

Obesity-related asthma and its relationship with microbiota

“…In experiment 2, CLA fed animals had body weights similar to restricted animals and CLA significantly reduced body fat (significantly lower than prior to and post restriction, or pair fed). This suggests that CLA exerted modulation of body fat independent of reduced food intake, CLA may be more effective at protecting against fat mass regain following weight loss than as a loss treatment (Park et al, 2007). Bifidobacterium, Bifidobacterium pseudolongum, and Bifidobacterium breve can convert free linoleic acid and a-linolenic acid to different CLA (Yeganeh et al, 2017).…”