Influence of storage conditions and multiple freeze-thaw cycles on N1 SARS-CoV-2, PMMoV, and BCoV signal

Thapar, Isha; Langan, Laura M.; Davis, Haley; Norman, R. Sean; Bojes, Heidi K.; Brooks, Bryan W.

doi:10.1016/j.scitotenv.2023.165098

Cited by 4 publications

(1 citation statement)

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“…Only seven samples were positive using sequencing, which may be attributed to RNA decay, due to the sequencing being performed 1.5 years after sample collection and RNA extraction. During that time, the RNA eluents were stored at −80 °C and have been freeze-thawed multiple times, which can result in some loss of RNA [ 43 ]. It is also possible that the sample concentration and extraction steps applied for the passive samplers resulted in RNA fragmentation, which then prevented the RT and amplification of 3.9 kb of RNA sequences.…”

Section: Discussionmentioning

confidence: 99%

Near-source passive sampling for monitoring viral outbreaks within a university residential setting

Farkas,

Kevill,

Adwan

et al. 2024

Epidemiol. Infect.

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Wastewater-based epidemiology (WBE) has proven to be a powerful tool for the populationlevel monitoring of pathogens, particularly severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For assessment, several wastewater sampling regimes and methods of viral concentration have been investigated, mainly targeting SARS-CoV-2. However, the use of passive samplers in near-source environments for a range of viruses in wastewater is still under-investigated. To address this, near-source passive samples were taken at four locations targeting student hall of residence. These were chosen as an exemplar due to their high population density and perceived risk of disease transmission. Viruses investigated were SARS-CoV-2 and its variants of concern (VOCs), influenza viruses, and enteroviruses. Sampling was conducted either in the morning, where passive samplers were in place overnight (17 h) and during the day, with exposure of 7 h. We demonstrated the usefulness of near-source passive sampling for the detection of VOCs using quantitative polymerase chain reaction (qPCR) and next-generation sequencing (NGS). Furthermore, several outbreaks of influenza A and sporadic outbreaks of enteroviruses (some associated with enterovirus D68 and coxsackieviruses) were identified among the resident student population, providing evidence of the usefulness of nearsource, in-sewer sampling for monitoring the health of high population density communities.Key findings -Wastewater surveillance is feasible for small, high-density communities -Passive sampling is a low-cost, simple approach for building-level wastewater monitoring -Enteric and respiratory pathogens can be monitored in sewers quantitatively via quantitative polymerase chain reaction (qPCR) -Viral variants/strains can also be identified in sewers via sequencing Epidemiology and Infection www.cambridge.org/hyg

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Section: Discussionmentioning

confidence: 99%