Influence of substrate and microbial interaction on efficiency of rumen microbial growth

Demeyer, Daniël; Nevel, Christian Van

doi:10.1051/rnd:19860203

Cited by 64 publications

(35 citation statements)

References 46 publications

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“…The values proposed by Church (1988) (Baldwin et al, 1987;Danfaer, 1990;Dijkstra, 1993). In fact the estimations of this requirement varies within a large range of values (Belaich, 1986;Demeyer and van Nevel, 1986). However, it should be borne in mind that most of these available data result from in vitro studies and that their application for in vivo use is problematic.…”

Section: Discussionmentioning

confidence: 99%

“…Asumptions of 90% organic matter in the microorganisms and a microorganism 'molecular weight' of 162 g, equal to that of a mole of carbohydrate were made. For the bVFA, capture is supposed to be total since the microorganisms seem to be unable to synthesise the branched-chain carbon skeletons (Andries et al, 1987 (Sauvant, 1992b (Isaacson et al, 1975;Demeyer and van Nevel, 1986). The costs of microbial dry matter synthesis (Church, 1988, …”

Section: Methodsmentioning

confidence: 99%

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Development of a mechanistic model for rumen digestion validated using the duodenal flux of amino acids

Lescoat¹,

Sauvant²

1995

Reprod. Nutr. Dev.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Development of a mechanistic model for rumen digestion validated using the duodenal flux of amino acids

Lescoat¹,

Sauvant²

1995

Reprod. Nutr. Dev.

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“…Rumen metabolism can be characterized by a fermentation pattern, consisting of the following parameters: the amount of molar proportions of volatile fatty acids produced; the amount of methane formed and OMF (Demeyer and Van Nevel, 1986). All these parameters of rumen fermentation were determined in this study.…”

Section: Effect Of Modifying N-6/n-3 Ratio Of Dietary Oil Supplementsmentioning

confidence: 99%

Effect of microbial oil and fish oil on rumen fermentation and metabolism of fatty acids in artificial rumen

Jalč¹,

Čertı́k²,

Kundrikova³

et al. 2009

CZECH J ANIM SCI

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The objective of this study was to examine the effect of microbial oil (MO, n-6 fatty acids) and fish oil (FO, n-3fatty acids) used in their blends as supplements (5% wt/wt) to the diet containing 80% of hay and 20% of barley on rumen fermentation and lipid metabolism in artificial rumen. Overall, three different ratios of n-6 and n-3 fatty acids (1:1, 3:1, and 5:1) as the blends of MO and FO were used. Two similar consecutive experiments were carried out within 2 months. Each experiment lasted for 12 days with 6 days of stabilization period. The addition of all three oil blends did not affect the parameters of fermentation such as degradation of dry matter (DM), detergent fibre, total gas production, but increased the degradation of cellulose and hemicellulose in the diets. The supplementation of oil blends to the diet insignificantly (NS) decreased the methane production (mostly the n-6/n-3 ratio 1:1, about 23.5%), increased (P < 0.01) mol% of propionate (mostly the n-6/n-3 ratio 1:1, about 24.1%) and decreased (P < 0.05) mol% of acetate (mostly the n-6/n-3 ratio, 1:1, about 7.7%). The lipid metabolism in artificial rumen was also affected, when the oil blends increased (P < 0.001) the concentration of total fatty acids (FA) and long-chain FA (LCFA) in effluent. The concentration (mg/g rumen fluid DM) of trans (trans 11 C18:1, TVA-vaccenic acid), cis C18:1 isomers and CLA-conjugated linoleic acid (cis 9, trans 11 C18:2) was also increased (P < 0.001) by the oil blends. Finally, the oil blends caused the incomplete FA biohydrogenation by an increase in TVA concentration and TVA/C18:0 ratio in effluent in artificial rumen.

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“…In general, defaunation resulted in a considerable reduction (20-50%) in rumen methane production . This might be due to (1) a loss of some methanogens that are attached to ciliates, (2) reduced hydrogen production and/or (3) improved microbial growth efficiency (Demeyer and Van Nevel 1986;Van Nevel and Demeyer 1996). The latter may induce increased incorporation of rumen degraded OM in microbial cells, while the percentage partitioned into CH 4 will be reduced (Leng and Nolan 1984;Beever 1993).…”

mentioning

confidence: 99%

Methane emissions from the rumen of sheep fed a mixed grass-clover pasture at two fertilisation rates in early and late season

Mbanzamihigo¹,

Fievez²,

Gomez³

et al. 2002

Can. J. Anim. Sci.

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. Methane emissions from the rumen of sheep fed a mixed grass-clover pasture at two fertilisation rates in early and late season. Can. J. Anim. Sci. 82: 69-77. In vivo CH 4 production from four rumen-fistulated sheep fed fresh ryegrass-white clover (Lolium perenne -Trifolium repens) diets ad libitum was monitored using C 2 H 6 as a tracer gas. Treatments comprised mixed pastures at two different fertilisation rates (0 vs. 150 kg N ha -1 yr -1 ) and at two periods in the growing season (early, June-July vs. late, August-September). Higher crude protein (CP) [22.5% dry matter (DM)] in late vs. early season (13% DM) was associated with increased importance of protein fermentation, as suggested by higher rumen concentrations of iso-acids [3.7 ± 1.0 vs. 2.4 ± 0.9% total volatile fatty acids (VFA)]. However, rumen CH 4 production (25.8 ± 5.0 L kg -1 DMI) was not affected, probably because of compensatory seasonal changes in the chemical composition of the pasture, su0 as reduced water-soluble carbohydrates (WSC) (13.0 ± 2.6 vs. 4.9 ± 1.4% DM) or increased neutral detergent fibre (NDF) (48.5 ± 6.1 vs. 57.4 ± 4.6% DM). Although clover saponins might have a defaunation and CH 4 inhibitory effect, considerable seasonal or fertiliser-induced changes in clover proportion did not affect rumen protozoal concentrations (8.8 ± 1.6 × 10 5 mL -1 rumen contents, early season), rumen methanogenesis or microbial growth as suggested from urinary excretion of purine derivatives (6.7 ± 1.7 mmol d -1 ). In contrast, however, a clear animal effect was found: on average, one animal produced less CH 4 than the three others (20.3 vs. 27.6 L kg -1 DMI).Key words: Ryegrass, clover, N fertilisation, season, rumen, methane Mbanzamihigo, L., Fievez, V., da Costa Gomez, C., Piattoni, F., Carlier, L. et Demeyer, D. 2002. Les émissions ruminales de méthane par des moutons alimentés avec un pâturage mixte de rye grass-trèfle blanc à deux niveaux de fertilisation et au début et à la fin de l'été. Can. J. Anim. Sci. 82: 69-77. La production de CH 4 in vivo était mesurée sur 4 moutons fistulés au niveau du rumen et alimentés ad libitum avec de l'herbe fraîche en utilisant l'C 2 H 6 comme gaz traceur. Des pâturages mixtes (rye grass, Lolium perenne / trèfle blanc, Trifolium repens) à deux niveaux de fertilisation (0 vs. 150 kg N ha -1 an -1 ) ont été étudiés au début (Juin-Juillet) et à la fin (Août-Septembre) de l'été. Le pourcentage de PB plus élevé à la fin qu'au début de la saison estivale (22.5 % vs. 13 % matière sèche) était associée avec une fermentation protéique plus importante, comme le suggèrent les pourcentages plus élevés des iso-acides dans le rumen (3.7 ± 1.0 vs. 2.4 ± 0.9 % AGV totaux). Néanmoins, la production ruminale de CH 4 (25.8 ± 5.0 L kg -1 matière sèche ingérée) n'a pas changé, probablement à cause des effets compensatoires, de la diminution du taux de WSC (13.0 ± 2.6 vs. 4.9 ± 1.4 % matière sèche) ou de l'augmentation du NDF (48.5 ± 6.1 vs. 57.4 ± 4.6 % matière sèche) observées à la fin de la saison. Malgré la defaunation potent...

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Influence of substrate and microbial interaction on efficiency of rumen microbial growth

Cited by 64 publications

References 46 publications

Development of a mechanistic model for rumen digestion validated using the duodenal flux of amino acids

Development of a mechanistic model for rumen digestion validated using the duodenal flux of amino acids

Effect of microbial oil and fish oil on rumen fermentation and metabolism of fatty acids in artificial rumen

Methane emissions from the rumen of sheep fed a mixed grass-clover pasture at two fertilisation rates in early and late season

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