Influence of substratum hydrophobicity on salivary pellicles: organization or composition?

Abstract

Different physico-chemical properties (eg adsorption kinetics, thickness, viscoelasticity, and mechanical stability) of adsorbed salivary pellicles depend on different factors, including the properties (eg charge, roughness, wettability, and surface chemistry) of the substratum. Whether these differences in the physico-chemical properties are a result of differences in the composition or in the organization of the pellicles is not known. In this work, the influence of substratum wettability on the composition of the pellicle was studied. For this purpose, pellicles eluted from substrata of different but well-characterized wettabilities were examined by means of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that substratum hydrophobicity did not have a major impact on pellicle composition. In all substrata, the major pellicle components were found to be cystatins, amylases and large glycoproteins, presumably mucins. In turn, interpretation of previously reported data based on the present results suggests that variations in substratum wettability mostly affect the organization of the pellicle components.

Effects of CO2laser irradiation on matrix-rich biofilm development formation–an in vitro study

“…The formation and composition of biofilm appear to vary on different surfaces (Aroonsang et al, 2014) and effects of material/surface properties, such as surface charge, hydrophobicity, roughness, topography, and chemistry on bacterial adhesion and biofilm formation have been investigated for many years (Anselme et al, 2010; Badihi Hauslich et al, 2013; Guégan et al, 2014; Perera-Costa et al, 2014; Song & Ren, 2014). These factors may be interrelated, which may explain the inhibition of biofilm formation found on irradiated enamel.…”

Effects of CO2 laser irradiation on matrix-rich biofilm development formation – An in vitro study

“…However, with the progression of time, at day 5, this difference becomes less visible and is statistically similar. The formation and composition of biofilm appear to vary on different surfaces (Aroonsang et al 2014) and effects of material/surface properties, such as surface charge, hydrophobicity, roughness, topography, and chemistry on bacterial adhesion and biofilm formation have been investigated for many years (Anselme et al ; Badihi Hauslich et al ; Guegan et al ; Perera-Costa et al ; Song & Ren). These factors may be interrelated, which may explain the inhibition of biofilm formation found on irradiated enamel.…”

Comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteins

“…Serum proteins are able to attach to the tooth surface but they detach within a few hours after attaching [8]. When pellicle proteins recovered from different substrata were compared, pellicle protein deposited on a silica surface had a similar protein composition to that of pellicle deposited on natural teeth [9].…”