Influence of Sulfate on Methane Fermentation in a Defined Medium

Minami, Kiyoshi; Tanimoto, Yuichi; Tasaki, Masaharu; Ogawa, Shigemichi; Okamura, Kazuo

doi:10.2166/wst.1988.0323

Cited by 6 publications

(1 citation statement)

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“…황산염이 결핍된 경우를 제외한 일반적인 해양퇴적환경에서, 메탄 생성기작에 의한 최종 유기물 분 해는 총 유기물 분해량의 10% 미만으로, 황산염 환원 박 테리아의 황산염 환원활동이 거의 완료된 후에 시작된다 (Martens and Berner 1974;Oremland and Polcin 1982;Capone and Kiene 1988). 이때에는 메탄 생성 미생물들과 황산염 환원 박테리아가 H 2 나 acetate 등과 같은 전자 공 여체 수용을 연속적으로 또는 동시에 공통 기질에서 경쟁 하여, 메탄 생성 미생물의 호흡기작이 강화되면 반대로 황 산염 환원 박테리아의 활성은 감소한다 (Minami et al 1988; Koster et al 1986). 혐기성 환경에서 독성 유기물질 의 분해는 서식 미생물들이 다양한 전자 수용체를 이용하 는 데 있어 퇴적환경의 산화환원 전위, pH 등과 같은 물 리화학적 환경차이 등에 따라 상대적인 차이가 있을 수 있다 (Beller et al 1992).…”

Section: 서 론unclassified

Organic Matter Cycle by Biogeochemical Indicator in Tidal Mud Flat, West Coast of Korea

Lee¹,

Lee

Jeong³

et al. 2014

Ocean and Polar Research

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: To understand the degradation processes of organic matter related to sulfate reduction by Sulfate Reduction Bacteria (SRB) in the tidal flat sediments of Hwang-do and Sogeun-ri, Tae-an Peninsula in Chungnam-do, biogeochemical characteristics were analyzed and highlighted using specific microbial biomarkers. The organic geochemical parameters (TOC, δ 13C org , C/N ratio, long-chain-n-alkane) indicate that most of the organic matter has been derived from marine phytoplankton and bacteria in the fine-grained sediment of Sogeun-ri, although terrestrial plant components have occasionally been incorporated to a significant degree in the coarse-grained sediment of Hwang-do. The concentration of sulfate in pore water is a constant tendency with regard to depth profile, while methane concentration appears to be slightly different with regard to depth profile at the two sites. Especially, the sum of bacteria fatty acid (a-C15:0 + i-C15:0 + C16:1w5) confirms that the these concentrations in Sogeun-ri are related to the degradation of Benzene, Toluene, Ethylbenzene and Xylene (BTEX) compounds from the crude oil retained in the sediments as a result of the Hebei Spirit oil-spill accident in 2007. The methane-related microbial communities as shown by lipid biomarkers (crocetane, PMI) are larger in some sedimentary sections of Hwang-do than in the Sogeunri tidal flat. These findings suggest that methane production by microbiological processes is clearly governed by SRB activity along the vertical succession in organic-enriched tidal flats.

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Section: 서 론unclassified

Organic Matter Cycle by Biogeochemical Indicator in Tidal Mud Flat, West Coast of Korea

Lee¹,

Lee

Jeong³

et al. 2014

Ocean and Polar Research

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Pathways of methanol conversion in a thermophilic anaerobic (55 °C) sludge consortium

Paulo

Stams

Field

et al. 2003

Appl Microbiol Biotechnol

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The pathway of methanol conversion by a thermophilic anaerobic consortium was elucidated by recording the fate of carbon in the presence and absence of bicarbonate and specific inhibitors. Results indicated that about 50% of methanol was directly converted to methane by the methylotrophic methanogens and 50% via the intermediates H(2)/CO(2) and acetate. The deprivation of inorganic carbon species [Sigma(HCO(3)(-)+CO(2))] in a phosphate-buffered system reduced the rate of methanol conversion. This suggests that bicarbonate is required as an electron (H(2)) sink and as a co-substrate for the efficient and complete removal of the chemical oxygen demand. Nuclear magnetic resonance spectroscopy was used to investigate the route of methanol conversion to acetate in bicarbonate-sufficient and bicarbonate-depleted environments. The proportions of [1,2-(13)C]acetate, [1-(13)C]acetate and [2-(13)C]acetate were determined. Methanol was preferentially incorporated into the methyl group of acetate, whereas HCO(3)(-) was the preferred source of the carboxyl group. A small amount of the added H(13)CO(3)(-) was reduced to form the methyl group of acetate and a small amount of the added (13)CH(3)OH was oxidised and found in the carboxyl group of acetate when (13)CH(3)OH was converted. The recovery of [(13)C]carboxyl groups in acetate from (13)CH(3)OH was enhanced in bicarbonate-deprived medium. The small amount of label incorporated in the carboxyl group of acetate when (13)CH(3)OH was converted in the presence of bromoethanesulfonic acid indicates that methanol can be oxidised to CO(2 )prior to acetate formation. These results indicate that methanol is converted through a common pathway (acetyl-CoA), being on the one hand reduced to the methyl group of acetate and on the other hand oxidised to CO(2), with CO(2) being incorporated into the carboxyl group of acetate.

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